FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45820.238621299160407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG271.1931064958020325No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG200.8837825894829873No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT180.7954043305346884No Hit
GTATTTATTTGTGGGGTAAATTAATAAGCATTCCAGATGCTTGCAGCTTCCTGCATCCAGGAGATGCTGTG150.6628369421122404No Hit
AGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGT130.5744586831639417No Hit
TACGCAGATTTCTACAGAAACTACGATGTCATGAAAGATTTTGAGGAGATGAGGAAGGCTGGTATCTTTCA110.4860804242156429No Hit
GGGACAGGTGAATTTACTACTTTCTAAAGTTTATTGATACTTCCCTTTTATGTAAAATGTAGTAGTGATAC100.44189129474149363No Hit
GCCTGGAATGAGCTGAAAGGGACTTCCAAGGAAGATGCCATGAAAGCTTACATCAACAAAGTAGAAGAGCT100.44189129474149363No Hit
GCTGCATCCCGCGTCCAGCACCTACGTCCCGCTGCCGTCGCCGCCGCCACCATGCCCAAGAGAAAGGCTGA100.44189129474149363No Hit
GTTTTAGTGCTTATGTTTAGGTTTCTGATGCATTTTTAGTTCATTTTTCTTTATGATCATTTTTTTTTCTT100.44189129474149363No Hit
GGATTCAAATGGGGATTTGCTGCATTTGTGGTAGCTGTAGGAGCTGAATATTACCTGGAGTCCCTGAATAA100.44189129474149363No Hit
GATCCAAGATAAAGAAGGCATCCCCCCCGACCAGCAGAGGCTCATCTTTGCAGGCAAGCAGCTGGAAGATG90.3977021652673442No Hit
CAGTGTCACTGTGCCACAGTCTATTGGTCAGCTGCATATAGGACTGACCTAGGTTCATGGGGAAGGAACAC90.3977021652673442No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA80.3535130357931949No Hit
GGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGGAGATAAAGTTCTTCTCCCAGAATATGGAGGCACCA80.3535130357931949No Hit
GCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAA80.3535130357931949No Hit
CATATACTCTGCAGCTGTTAGAATGTGCAAGCACTTGGGGACAGCATGAGCTTGCTGTTGTACACAGGGTA80.3535130357931949No Hit
CTTCTTGCAGAAGGGAGGGGGAAACATACATTTATTCATGCCAGTCTGTTGCATGCAGGCTTTTTGGCTTC80.3535130357931949No Hit
GAAAATAGAAACAAATGAGAAACATTACATGCTCATGGATAGGAAGAATAAATATTGTGAACATGGCCATA80.3535130357931949No Hit
GTTAAATATTGGAAGCTGTGTGTAAGATTGATTCAGCATTCCATGCACTTGCTTTAAAATTTAGTCCTGTG80.3535130357931949No Hit
GGCTGCAGACCATCTGGAAGGAAGAGCCCATCCCCTGCACAGCCCACTGGCACTTCGGGCAATAACTCTGT80.3535130357931949No Hit
CCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.30932390631904555No Hit
TCATTGCCTTAACTGGAGCCCCATCAGCTCCAGCTGCTCTGTCTTCTTTGCCCACAATGCTTTGCCCCTGA70.30932390631904555No Hit
CCTTAATCCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGACAACACATAAAAAAAAAAA70.30932390631904555No Hit
CTGTCCTAGGGATTGGGCCAGGACCAGCAGACCTAATTGACAAAGTCACTGGTCACCTAAAACTTTACTAG70.30932390631904555No Hit
GAAGCCAAGGTCATGTGCATGAAATGAGGAGTTTGAGTTAGTCACCTCGGGGATTTTTTCCATTTTGCAGT70.30932390631904555No Hit
GAAGAAAAGCCAGAACCAGACATAAGTTCAGAGGAATCTGTCTCCACTGTAGAAGAACAAGAGAATGAAAC70.30932390631904555No Hit
GTGCCTGGCTTGAGGAAGCATTTTAAAAAATTACTGTCATTAGTGTGGGATGGTGAAGTATGCAAAGTATA60.26513477684489617No Hit
CTGGGACTTGTTAAATTCCTATTGATTAGTAGATACAAGTTGACCTTTCTCTGGCCCCCAGCTCTAGTGTT60.26513477684489617No Hit
CCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGGAGAACCTGAA60.26513477684489617No Hit
GCTTGCAGTGAGCCGAGATTGTGCCACTGCACTCCAGCCTGGACAGAAATGCATTTCATAATGCATTTTAA60.26513477684489617No Hit
ACTATACCTGTCTGACACAATATCACCAGAGCAAGCCATAGACAGCCTGCGAGACCTAAGAGGATCCGGGG60.26513477684489617No Hit
GTCTTAAACATTTCTAAAAGTGGTTCTTGCAGTGCTATACGTATTGGTCACGTTGGAATGCAAATTGAGCA60.26513477684489617No Hit
CCTTAAGGTCTGATGTTATGGCAACAAACTACTTTTTCAAACCTAAATAGGAACCATGTAATTTCTCAAAA60.26513477684489617No Hit
TTTCTACTCCCTCCCCCCAATATAAAAATATAGACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.26513477684489617No Hit
ACTTATGTTTTCCATGTTGATTGAGGGAGGCAATTTTATGGATACCAATTAATCTTGAGATCCTGAACATG50.22094564737074682No Hit
GAAGAAAGCAGAGCCCAGCAGAAGGAGACCTTGAAATCTCTTCTTGAACAAGAGACAGAAAATTTGAGAAC50.22094564737074682No Hit
GGCACCATCAGCCATCATGACATGTATGATTACCTGCATCTGAGCCGCCTGGGCTACACACCTGTTTGCCG50.22094564737074682No Hit
GCATTACGTGAGCTGGTCCCGTGTACAATCCCCAAGCCACGGCAAAACATCAGGAGAACGCACCGATCTCA50.22094564737074682No Hit
CATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGG50.22094564737074682No Hit
GTCCAGCCTATGGCCTTTCTCCTTTTGTCTCTAGTTCATCCTCTAACCACCAGCCATGAATTCAGTGAACT50.22094564737074682No Hit
GGTGAACGTTTTAACAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.22094564737074682No Hit
CATAGGAATGCTGGACCAGGGTACCAGATTTTTTCAACAAAGGGGGTGAAGTGTCCTACTAAAAAGAATAA50.22094564737074682No Hit
CCAACAGTGATGGTCAGCTAGATTTCTCAGAATTTCTTAATCTGATTGGTGGCCTAGCTATGGCTTGCCAT50.22094564737074682No Hit
CAAGAGGTGAGCACTTAACAATAGAGTTTGAGAATCTAGTAGAAAGTGATGAAGGGGAGAGCCCAGGAAGC50.22094564737074682No Hit
GTCAACGATTAGTCATGCAATAAATGTTCTGGTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.22094564737074682No Hit
GTTTTTTAATAAATGTTCCTGGTTGGTTTTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.22094564737074682No Hit
GGAGAAAAAGCTGAACCAGGCCCTTTTGGATCTTCATGCCCTGGGTTCTGCCCGCACGGACCCCCATCTCT50.22094564737074682No Hit
CAACCAAAATCTTATCATGGTCTCAGTCATAATCATTAGGGGGAACTCTAGCCAAATGGTTTAACTTCTGC50.22094564737074682No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17675651789659744No Hit
GTCTTGGCGATTCGGCCCGACGAGCTCCGCTTTCGCTACAGCATGGTGGCCTACTGGAGACAGGCTGGACT40.17675651789659744No Hit
AAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGAGATCTGGACAGAATCGCCGGACAGGTGGCAGCT40.17675651789659744No Hit
GCTCTTCCGATCTCGAAGAATCCAAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17675651789659744No Hit
GCCTGGCCAACATGGTGAAACCCCATGTCTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17675651789659744No Hit
AGCAAAATCATGGAATGGTTATAAAAGTGATTGTTGGCACATCCTATGCAATATATCTAAATTGAATAATG40.17675651789659744No Hit
AAATATAATCACTGCTGTGATAAATAAACTATCTATTGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17675651789659744No Hit
GCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCT40.17675651789659744No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17675651789659744No Hit
CTATAACGGCGTTGCCCTGCGGCCCTGGGACGGCAACTCTGATGACCGGGTCTTGTTGGATCTGTCTGCCT40.17675651789659744No Hit
GACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAAAAAAAAAAAAAGAAAAAAAAA40.17675651789659744No Hit
CGTCGCCCACTGCTGCTGCTGCAGCTCCCCCGGGCCCCGCAGGGAAAGCCCAGGAAGGTGAGCCCCTGGAA40.17675651789659744No Hit
GTCCTCATCTCCCTCAAGCAGGCCCCGCTGGTGCACTGAAGAGCCACCCTGTGGAAACACTACATCTGCAA40.17675651789659744No Hit
CAACTATAGTTAACTAGTATCACATGTTTAGTTGGTATGTAAACTAATGTATCCAGAAGATTGGAAGAGGG40.17675651789659744No Hit
TCCCTACACGACGCGGGGATCCATATTTAGTTTTATAAGCTTTTCCCTGTTTTTAGTTTTGTTTTGGGTTT40.17675651789659744No Hit
GTGCTGTTGTTTAAAATAAAAGTACCTATTGAACATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17675651789659744No Hit
TTTTGGAGCCTTCCTGAAGGTGTAAAATTGTAAATATTTTTATCTATGAGTAAATGTTAAGTAGTTGTTTT40.17675651789659744No Hit
GGCTACCTGCTGGGCCCACATGCCGTTGGCAACCACAGGTCATTCAGCGACAAGAATGGCCTCACCAGCAA40.17675651789659744No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAG40.17675651789659744No Hit
GGCCTCTGAGCAAGACAGTGCGCTTCAACGTGCTCAAGGTCACCAAGGCTGCCGGCACCAAGAAGCAGTTC30.13256738842244808No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC30.13256738842244808No Hit
CACATTGCTGGTCAGCCAATCAGATGGTGGTAATGAGGGAAAAACAAATAATTACTGCCACCTGGATTCTC30.13256738842244808No Hit
TTATATATTCTTATAAAAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13256738842244808No Hit
CTGGCTATCATTGATCCAGGTGACTCTGACATCATTAGAAGCATGCCAGAACAGACTGGTGAAAAGTAAAC30.13256738842244808No Hit
GTGAGCCGAGATCCCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAA30.13256738842244808No Hit
CATCCGAGCCAGCCAAGGAGGAGGATGATTACGACGTGATGCAGGACCCCGAGTTCCTTCAGAGTGTCCTA30.13256738842244808No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13256738842244808No Hit
TTTTATATCTGTTCAATTTTTATCATTCCCCTTATCATTAGAAAGTTTGACAAAAATACTTGAATCTTGGA30.13256738842244808No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAAAAAAAAAAAAAAAAAAAA30.13256738842244808No Hit
AAGAACCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCGAACCCCCCAAAGCTGGTTTCAAGCCAA30.13256738842244808No Hit
CCTCTATAAAATGTGTACTGGGAATAAGCTTTGTATTTACATACATTAGGGGAATTTTTTAAAATCTGTAA30.13256738842244808No Hit
CACCTAAAAAATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13256738842244808No Hit
ATTATTGTGGAAAATACAGCATAAATGAAAATTCATAAAAATATAAGCTATGAGCTAATTTCCAAATATAC30.13256738842244808No Hit
ACGCTCTTCCGATCTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13256738842244808No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13256738842244808No Hit
CCTGTGACATTCTGGACTATTTCTGTGTTTATTTGTGGCCGAGTGTAACAACCATATAATAAATCACCTCT30.13256738842244808No Hit
AACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAA30.13256738842244808No Hit
GAACTTTTGTTAGCAAGGAGGTTTCAGAAATGATTTTGGATTTTCTGTAAGTGTTTAATTTAGTTCTAGGG30.13256738842244808No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT30.13256738842244808No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC30.13256738842244808No Hit
GGGGAAGGGAGGGGAGACTATGTGTGAGCTTTTTTTAAATAGAGGGATTGACTCGGATTTGAGTGATCATT30.13256738842244808No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC30.13256738842244808No Hit
ATCCCAGCTACTCTGGAGGTTGAGGCAGGGGAATCGCTTGAACCTGGGAGCCAGAGGTTGCAGTGATCTAA30.13256738842244808No Hit
GCACTTTGGGAGGCCGAGGCGGGCAGATCGCAAGGTGAAGAGATGGAGACCATCCTGCCCAACATGGTGAA30.13256738842244808No Hit
ATCCCATTTCTACTAAAAATACAAAAATTAGCTGGGCGTGGTGGCAGGCGCCTGTAATCCTAGCTACTTGG30.13256738842244808No Hit
TATTAAAGCAAGGAAAAGTGTAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13256738842244808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)