FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70022.7125243348475No Hit
CAGTATAGCTTTTAAATTTTAAAGCATTTGGTAAATTATCGCTGAGTTTTTTTCTGTTGCCAATAGCAAAC250.8111615833874107No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG240.7787151200519143No Hit
GTACTCGGAAAACCCCTGCCAAAATCAGGATCTGTCTTCATTGGTGGATTTCCAGACCTTCATGGGAAAAT180.5840363400389358No Hit
TTTCTAAAGAGTCTTGTTATTTCTTCTTTGAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTC150.48669695003244645No Hit
CTAATGAGGAAAATGGGGAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGAGGAA140.45425048669695006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.45425048669695006No Hit
GGGTCTTTGCTTCTGATTACAGGAGCACTGTGGAACGTCTGTAAATATGTTTTTATAATTCCATGTATAGT130.42180402336145356No Hit
GATTTTTTTCTGCGTTGTTTAATGCCTGAAATCCAAGTCTTCCTCCATGGGAAAATACTGTTATACCAAAT120.3893575600259572No Hit
GAATTGGGATTTACCAATTATATTCGAGCCCTCCGGGAGGGAGACCATGAAAGACTAGGGAAAAAGAAGGA120.3893575600259572No Hit
ATACCCATTAAGACTAGTACTTACACAGTGGACATGAAAAAACTAGGCAGCAAGGAGTTATTAAAAAGAAA110.3569110966904607No Hit
GCATTCTAGAGTGCATATATTTATATTTTGCCTGTTAAAAAGAAAGTGAGCAGTGTTAGCTTAGTTCTCTT110.3569110966904607No Hit
CTTTATTGCTCTAAAATTAGTAACATTTGGCCAGGCTCGGTGGCTCACGCCTGTAATACCAGCACTTTGGG110.3569110966904607No Hit
AGCTAATATTTCCAGAAAGAATTGATCCTAACCCAGCAGACTCACAAAAAAGTACACAAGTGGAATAAAAT110.3569110966904607No Hit
GTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTTTTGTCTATGAAGTTGCTGTTTATTTTTT110.3569110966904607No Hit
GCTTTTAAGAAGTACCTGCAAGAAACCTACTCAAAGCAGGTCACCAGCCAGGAGCCCTGACCCAGGCTGCC110.3569110966904607No Hit
GTCAATCCAATACTGCTCCCAAATCCTGTTTTCAGTGTTCATTTCCCTCAAGGCAGGCGCTGGGCTCCCAC110.3569110966904607No Hit
GGGATCATGTGTTTAAAAAAAGAAAGGCTACGATGACTGGGCAAGAAGAAAGATGGGAAACTGAATAAAGC100.32446463335496434No Hit
GTTCGAGACCAGCCTGGGCAACATGGCAAAACCTCATCTCTACAAAAAACACAAAAATTAACCAGACGTAG100.32446463335496434No Hit
TCTCTCAATCCTCTGATCAAATATCTGAGTGCCACCACTGGCTTTGGAAGAAATTATATTATGACCCAGAA100.32446463335496434No Hit
AGATGGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCCTGGGAGACAGTGATAAT100.32446463335496434No Hit
ATTATGTCCTACAAGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.32446463335496434No Hit
GCCAAGTACCCCGACTACGAGGTCACCTGGGCTAACGACGGCTACTGAGCACTCCCAGCCCGGGGCCTGCT100.32446463335496434No Hit
ACCCTGTCTCTACTAAAAATACAAAAATTAGCTGAGCGTGGTGGCATGCACCTGTAGTCCCAGCTACCCGG100.32446463335496434No Hit
GAATGACTCGACCAGACAGATAAGGATAGAGGGGAGGCCTTATTGTATCGGTTTTATATTACCTGTTCTGC90.2920181700194679No Hit
AGCATTGCCAATATCCTGTATTCTTGTTTTACATTTGGATTAGAAATTTAACATAGTAATTCTTAAGTCTT90.2920181700194679No Hit
GAGCAGCGCAAGATTGATCAGAAAGCTGTGGACTCACAAATTTTACCAAAAATCAAAGCTATTCCTCAGCT90.2920181700194679No Hit
GGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.2920181700194679No Hit
GAACTGCTGATACACTTTCATTCTCAGTAAATTAATTTTTTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA90.2920181700194679No Hit
TCTTTGCCTTCCTTACCTCGTGATCTCACCATTCCAGATTGAAATCATGGCAGGTCCAGAAAGTGATGCGC90.2920181700194679No Hit
GGCCTCACCCTCTGGCAGCTGAGCCAAGCACTGTCATTCTTGGTGCCATCTTCCCCTGCCGCACCGGCAGT90.2920181700194679No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA80.25957170668397145No Hit
CATTTTAAATCTGAATCTGAAGAGGAGAAAGCATTGATTTATCAGTTCAGTCCAATTTATACTGGAAATAT80.25957170668397145No Hit
TTGCCCGTGTTCTCACAGTTATTAACCAGACTCAGAAAGAAAACCTCAGGAAATTCTACAAGGGCAAGAAG80.25957170668397145No Hit
GCCTCAGCTTAATCCAGGAGACAAAGATTATTTTCCTTATTATCTCTTCTGCATAGGATCTGCAATCAGAA80.25957170668397145No Hit
GTGTTCCTCACCAATAACTTCAGAGAAGTCAGTTGGAGAAAATGAAGAAAAAGGCTGGCTGAAAATCACTA80.25957170668397145No Hit
TGATAATATTGAGGACAAAATCAAGACCTTTACAGATAAACTAGGACTCAGATAGTTTATACACAAGGTCT80.25957170668397145No Hit
CTAATAATCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.25957170668397145No Hit
CCACCATGCCTGGCTAATTTTTTGAATTTTTGTAGTGATGGGATCTCGCTCTGTTGCCCAGGGTGGTCTCG70.22712524334847503No Hit
CCTTGGGCACCACTGTCTTCTTGTGGATCTATCTCATCAAACAACACAATGAAGATATTTTAGAGTACAAA70.22712524334847503No Hit
GCATTGATAGCCAGTGTGTTTTCTTATTTAACTCCGTTTACTACATTCTACATGGTGTTTACGTGATCCAC70.22712524334847503No Hit
GTTGCAGATACTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.22712524334847503No Hit
GGCATGATGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATAGCTGGAACCTGGGA60.1946787800129786No Hit
GTACATAGGATACACGTTCAGTGTAAAATAAAGTGTAAAGGGGAATTCAGGCTTAATGCTGCACCTAGATA60.1946787800129786No Hit
GTGTCAATAAGTCTTTGCTTTCCAATCAACAGGGACGATTTTAATTATAGATTGTCTTCCTATTAAGTATG60.1946787800129786No Hit
GGTGAATTACGTGCCTCCATAACCTGAACTGTGCCGACTCCACAAAACGATTATGTACTCTTCTGAGATAG60.1946787800129786No Hit
GTTTTCTAGTTGTGCATGAATGCTGGCAACTTAGTAAGTTTTGACAATTGTTTAAATATGTAATGTTAAGC60.1946787800129786No Hit
CAATAAAATAAAATAAAAAAAAATTTCCAGGCCCAGACGTCTGTGGGAGTACGTGAGGAAAGGATTCCACT60.1946787800129786No Hit
CCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACG60.1946787800129786No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT60.1946787800129786No Hit
CCCTACACGACGCGGGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAAT60.1946787800129786No Hit
GCTCTTTTCAGTTTTTGCTTATACACAATTCATTCTTTGCAGCTAATTAAGCCGAAGAAGCCTGGGAATCA60.1946787800129786No Hit
GAGTAAATTCCATTTTTTTGAAGATCAGCTCCGTGGGGCTGGTTTTGGTCCACAGCATAACAGGCACTGCC60.1946787800129786No Hit
GAGCAAGACTATATCTCATATAAGAAAAAAAAAAAATAGAATTATAAAGCAAACACCCATATAACCCACCC60.1946787800129786No Hit
GGATGACATGAATCCTGGAAGGTCAAGGCATGAGCAAACCATGATCATCCCACTGCACTCCAGCCTGGGTA60.1946787800129786No Hit
TCCCTACACGACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1946787800129786No Hit
ATCTATAACCTTAGCTAGAGTGTCGCCTTGTGGGTTCCTGTTGCTGAGACTTCCTGGATGGAGCCGCCCTC50.16223231667748217No Hit
GGGGAGAAGGAGCTGCCGTGCGTCTTCCCTGTGCCCCGTCTCCCTGCTTGGTTCTCCCCTCCCTTCCCTGG50.16223231667748217No Hit
GTCCATACCTGAAAACAATATCATGCGCACAATCATTGAGTTTCTGTCTTTCTTGCATCTCAAAGAGGCCG50.16223231667748217No Hit
ATTGACAGAGAGGGGAAACCCCGTAAAGTGGTTGGTTGCAGTTGTGTAGTAGTTAAGGACTATGGCAAGGA50.16223231667748217No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTT50.16223231667748217No Hit
GGTCTATGCAATGTCAGTTTGGAATCTTCTTGAAAGTTTAATGTTTTTATTAGGAGATTTAAAGAAAATAA50.16223231667748217No Hit
TCCCTACACGACGCGGGGGCCACACGCCAGGCGCCACGCGCCGCCCTCGCTCTTTATCCACTGAGCAGCTT50.16223231667748217No Hit
GTATTAAGTAAATAAATAAGTAAATAACCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.16223231667748217No Hit
CTCTTATATCTATATTTTACATAAATTTAGTATTTTGTTTCAGTGCACTAATGTGTAAGACAAAAAGCTAC50.16223231667748217No Hit
CCCAACATGCTGCTGAGTGAAGAGGAAACTGAGAGTTGATTGTGCCAGGCGCCAGTTTTTCTTGTTATGAC50.16223231667748217No Hit
GGAAAGCAGACATTGACCTCACCAAGAGGGCGGGAGAACTCACTGAGGATGAGGTGGAACGTGTGATCACC50.16223231667748217No Hit
TCAATAAAGTTTGTGTTTATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.16223231667748217No Hit
CTCCTGCCATTACCAGCTCCCTTCTTGCAGAAGGGAGGGGGAAACATACATTTATTCATGCCAGTCTGTTG50.16223231667748217No Hit
AAACCATGAAAGACTGCTTTGTCTCTAGGCAAAGTCTTGAGCCAGTTTTAAATTAATTAAAGTTCACATTT50.16223231667748217No Hit
CTCATGATTTTGTTTCACGGAAACAAACTCGTTCTGCTGTCAATCTGAAAATGCCAGTGCTGTGCCTTGGA50.16223231667748217No Hit
AGAGTATACTGTATTATGTAATCTTGTAAACATGCTTGCTTTTGCATATATATGCATACCTATGTATTTAC50.16223231667748217No Hit
TGGCTCTCTCTGCCAGAATTGTGTGCACTCTGTAACATCTTTGTGGTAGTCCTGTTTTCCTAATAACTTTG50.16223231667748217No Hit
GGTGTGCATCACCTTCGACCAGGCCAACCTGACCGTCAAGCTGCCAGATGGATACGAATTCAAGTTCCCCA50.16223231667748217No Hit
GCTCAGTGCTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.16223231667748217No Hit
TATTTAACCTTTGTGTATGAAAAAGGCTTTTGGGCTATGACTCAAAATGCAGAGACAACCAAAGATTGACA50.16223231667748217No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGC50.16223231667748217No Hit
GCCTAAATAAAGCTTGGCAACTTTTTTTCCTTTTTTGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.16223231667748217No Hit
CGAGATGCCAGCTCTGGAGTTCTCGGTTGTTGTAGGAGGTTGGGTGTTTTCAAATGGTAAAGATGTTTTGA40.12978585334198572No Hit
CCCTTGGCCTTGAGCCACTTCGAACTTCGGGGATCTCACCTCTTCCTCAGGATGGGGAGCTCACTCCGAGA40.12978585334198572No Hit
GGCCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATGCGACGAACCTCTGAGCATCCTGGTGAGGAATAA40.12978585334198572No Hit
GTTACATTAAAGCCCTGTGTTAAGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit
GACTGATGCCTTTGTCTCTAATGAGCTAGATGACCCTGATGATCTGCAATGTAAGCGGGGAGAACATGTTG40.12978585334198572No Hit
GCACATTCTTCTGTCACCTGCTTGCGAACATATCTGCAATGCACCATCAAAGCCACACAGAGTGTCCCTGG40.12978585334198572No Hit
GCCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGGAGAACCTGA40.12978585334198572No Hit
CAGATGTTTGTTTTCAAAATACTTCTTTTTCATTATTGGTTTTAAAAAAGCATTATCCTTTTATCTCACAA40.12978585334198572No Hit
ACTGCACATCCACAGAGCAGAGATCTCAAAGATTATGCGAGAATGTCAGGAAGAAAGTTTCTGGAAGAGAG40.12978585334198572No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATG40.12978585334198572No Hit
ACAGTCAGAGGTTCATTTCCTCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit
GGTACTCGTACACTATTTTTTTGTACTGCTGGTCCTGTACCAGAAACATTTTCTTTTATTGTTACTTGCTT40.12978585334198572No Hit
ATCTTATACCAGGAAGTCAAATGAGAAGACATCAGTAAAAATATTCTGGCCAACTCATGTTTTATTTATCA40.12978585334198572No Hit
CTGTTATTGTGGTTGTGGTGATAGCAGTTGTTGCTGGAATTGTTGTGCTGGTTATTTCCAGAAAGAAGAGA40.12978585334198572No Hit
ACAAGGTGCTGACCATATTAAATGTTCAAGTTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit
CTTGGTGGTAGTGGTCCCCCGGGCCCAGCTGTCTTTTCTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit
GTCCTGGGCCGGAGGTGGCGCGAACCCTGTGCGGCCTAGAGTGGGCCTGGGATGCCTCAGCACTAATGATC40.12978585334198572No Hit
GGGTCCAGACACGGCACCTACGGAGAGCCACGGACCGAAGCCAGAGAGCCTTTCCTCTGCAAGTGGGACTG40.12978585334198572No Hit
CATATGATTTTATTTTTAAGACCATATTATATTTGGGTATCTACTAATATTTTGTATAAAGCAATTTTTTG40.12978585334198572No Hit
GTCTGCATTGGACCCTAAACTGGACTAGAAGAGGCATCTTCAAGGTTCATACGTTGTCCAGCTGTAAGTTC40.12978585334198572No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit
GAGCCCTGAGAAATAACACCACACATCTACAACCATCTGATCTTTGACAAACCTGACAGAAATAAGCAATG40.12978585334198572No Hit
AAAGAATAGCAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12978585334198572No Hit

[OK]Adapter Content

Adapter graph