FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428
Sequences flagged as poor quality0
Sequence length71
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44130.88235294117647No Hit
CCTAAGAAGCTGCTCATGATGGCTGGTATCGATGACTGCTACACCTCAGCCCGGGGCTGCACTGCCACCCT110.7703081232492998No Hit
GATGAAGATGAGGAAGCTGAGTCAGCTACGGGCAAGCGGGCAGCTGAAGATGATGAGGATGACGATGTCGA110.7703081232492998No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG100.700280112044818No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT100.700280112044818No Hit
GGTTCAATTCAGTGTATAACTGAACACACTCATCCATTTGTGCTTTTGTTTTTTTTTATGGTGCTTAAAGT100.700280112044818No Hit
GAGTCCTCTTCCTGCTGCTCTGGGTTGGCATCTACGTGTGAGCACCCAAGGGTAACAACCAGATGGCTTCA80.5602240896358543No Hit
GCATTTGGAGGGGAACCTGTTCCCGTCGGTCCCCAAATATCCCCTTCTTTTTATGGGGTTAAGGAAGGGAC80.5602240896358543No Hit
CCTATGTACCTGTTACCACTTTCAAAAATCTACAGACAGTCAATGTGGATGAGAACTAATCGCTGATCGTC80.5602240896358543No Hit
GCGGGTGGATCACTTGAGGTCCGGAGTTCGAGACCAGCCTGGCTAACATGGTGAAACCCTGTCTCTACTAA80.5602240896358543No Hit
CTTATTTTTGCCTGTGTATGGAGAGATTCAAGAAATAATTTTAAAACCGCATACATAATAAAAGACATTGT80.5602240896358543No Hit
GCAGTGAAACGCTCCATGAATTGGTATAACAAGACATAACACTAAATGAAATGATCAAAACCATAGGTAGC60.42016806722689076No Hit
GAATTCATTGTACCTAATAACTGAAGTAAAAATAACTAGTTGTTCAACTTTTCCTAAACTCAAATCTATTT60.42016806722689076No Hit
GATTTCTCTTTGGTTTTGAGGTTGAAGTGTCTTTGGCCCATTTGTAAGTCCCCATCCCTACCCTACACAAA60.42016806722689076No Hit
GTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAA60.42016806722689076No Hit
AGCTTCACCCCACGTGGCTTGGTTCCCAGGGACAGTCAGGGCCTCGGGGGCCCAGCTATGTACAACGAAGC60.42016806722689076No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA60.42016806722689076No Hit
ATTAAGATGGCAGGAATAGTCTCTTGGCAAGAGTTACAGAGTGTGGGAGCCAGGAAAAAAACAGAGAAAAT60.42016806722689076No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.350140056022409No Hit
GTTTACAAAAAAAAAACACATGGTTAAATGTTAAATTTTAATTAAGGCCCCCAAAAATTAAATATAACTTT50.350140056022409No Hit
GCATACAATAAAACTTAGTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.350140056022409No Hit
GAGGTTGGCTTGAAGCCTGTGTGGTATAGTCCCAAAGTTTTCATTGAAGGTGCTGATGCAGAGACTTTTTC50.350140056022409No Hit
GGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTG50.350140056022409No Hit
GCTTTGAAGGGCAAATGACAGAGGATAACATAGAAGTTGGAATCTGCAATGAAGCTGGATTTAGGAGGCTT50.350140056022409No Hit
GGAGGCTACAGTGAGCCAAGATCGCACCAATGCACTCCAGCCTGGGCGACAGAGTGAGATTCCATCTTAAA50.350140056022409No Hit
CCAACAGTGATGGTCAGCTAGATTTCTCAGAATTTCTTAATCTGATTGGTGGCCTAGCTATGGCTTGCCAT50.350140056022409No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA40.2801120448179272No Hit
GGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCAAAA40.2801120448179272No Hit
GCCTTTTAATGAGTGTTTTTTCTTTTTGTATATGTTACGTGTTTGTTGTTGTATTAAATAAAGAGGAATGT40.2801120448179272No Hit
GGCGCGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAAGGGTGTGAACCCGGGA40.2801120448179272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.2801120448179272No Hit
GAATGTCCGGGCCTTATATATCAAGAGCACCATGGGCAAGCCCCAGCGCCTATATTAAGGCACATTTGAAT40.2801120448179272No Hit
GTTTTTTGAAGACTGGCAATAAAGCTGTCCATTCAATTCCAAATACTGGTTTTAAGGTATAGCCACTGATA40.2801120448179272No Hit
CTCTTTCCCTACACGACGCGGGATTTTTTTAATTGGTTTTATGCTGCTTTGTTAGGACAGATGTGTTTTGA40.2801120448179272No Hit
GAAACTGGATCCTGGCAGCGAGGAGACCCAGACGCTGGTTCGAGAGTACTTTTCCTGGGAGGGGGCCTTCC30.21008403361344538No Hit
AAACACACCTCGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
ACGCGGGACACCCAGGTGAAATAAACAGCCATGTTGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
TTTCTGTAGTTATCTGCATAATTCTTGTTTTTCTTTCCATCTGGCTCCTGGGTTGACAATTTGTGGAAACA30.21008403361344538No Hit
GCAAGACAGTGCGCTTCAACGTGCTCAAGGTCACCAAGGCTGCCGGCACCAAGAAGCAGTTCCAGAAGTTC30.21008403361344538No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC30.21008403361344538No Hit
CACTGACCACCTCGTCAAGACCCACACCAGAGTCTCCGTGCAGCGGACTCAGGCTCCAGCTGTGGCTACAA30.21008403361344538No Hit
GTGTGGAAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
GTGCCCATGTGCTACTGACAAGTGCCTCAGCTTCCCCCCGGCCCGGGTCAGGCCGTGGGAGCCGCTATTAT30.21008403361344538No Hit
TCCCGGCACTTTGGGAGGCCGAGGCGGGCGGGTCACGAGGTCAGGAGATCGAGACCATCCTGACTAACATG30.21008403361344538No Hit
GCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAA30.21008403361344538No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
GCATTTGGATTTTTTTAAAACAAGTAAATTTCTTATTGATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
CGACGCGGGAAGGAATGCCTGTGCCAATAAAAGGTTTCTCCAACTTGAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
GCCCCGTCTGAGGGTGGCGATTAAAGTTGCTTCACATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
GCTTTAAATCTGTAAATAGTATACACTTTTTACTTTTACACACTTTCCTGTTCATAGCAATATTAAATCAA30.21008403361344538No Hit
AAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGC30.21008403361344538No Hit
GTCAAATTTCATGAAGAAAACCTCTGGCACATTGACAAATACTAAATGTGCAAGTATATAGATTTTGTAAT30.21008403361344538No Hit
GGTCACCAGCCAGGAGCCCTGACCCAGGCTGCCCAGCCTGTCCTTGTGTCGTCTTTTTAATTTTTCCTTAG30.21008403361344538No Hit
CTTTGGGGGGTCGAGGTGAGAGGATAGCATGTGGCCAGAAGTTCAAGACCAGCCTGGGCAACATAGCGAGA30.21008403361344538No Hit
GGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
GTTATGATTAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21008403361344538No Hit
CTTTAACATAAAGCTTTCTTAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
CCATTGCACTCCAGCCTGGGCAAAAAAGCGAAACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAACCAAA20.1400560224089636No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA20.1400560224089636No Hit
CTTGAAGAGGGGAGGGGCCTAGGGAGCCGCACCTTGTCATGTACCATCAATAAAGTACCCTGTGCTCAATC20.1400560224089636No Hit
GCCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCACATTATTTATAAAAAATATACAATTATA20.1400560224089636No Hit
ATTATTAGCCCTACTTTATCAGGTGCACACTGAGGCTCACAGACGTTAAATGCCCTCTAGAGTCACATCAA20.1400560224089636No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA20.1400560224089636No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATC20.1400560224089636No Hit
GCGCCGTCCCATCACAAGCCCGGGGAGGGATCCCGCCTTTGAAAATAAAGCTGTTATGGGTGTCATTGAAA20.1400560224089636No Hit
GGTCACCAGCCAGGAGCCCTGACCCAGGCTGCCCAGCCTGTCCTTGTGTCGTCTTTTTAATTTTTCCGTAG20.1400560224089636No Hit
AACTAAAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
TCCCTACACGACGCGGGATTATGGGTCACAAAAAAAAATAAATTTTTTAATTAAATTTTTTTTAACATTCC20.1400560224089636No Hit
CTTTAAGATTTGGTCACACTCTCACCTAGGTGAGCGCATTGAGCCAGTGGTGCTAAATGCTACATACTCCA20.1400560224089636No Hit
AAGTGTTACATGCAGTAACTAATTTAATTTTCTCAGCAGCCCTCTGGGGTAGGCTCCACTACAATCCACAT20.1400560224089636No Hit
ATATCAAACTACATTCTTCTGAAAGATGTTTCTATTATTTCTTAGGTCACTTCCATATATATTATGTATAG20.1400560224089636No Hit
ACTGTAAAATTCGAGCCATATAAATAAAACCTGTACTGTTCTAGTTGTTTTAATCTGTCTTTTTACATTGG20.1400560224089636No Hit
GATTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATA20.1400560224089636No Hit
GATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGGCTGCTGGGTCGGTCCCGCCCACAGCTGGGG20.1400560224089636No Hit
GTTCTGGGTCAGCTTCTTTTACCTCAATTTTATTTACAATAAATACTCTATAACCAAAAAAAAAAAAAAAA20.1400560224089636No Hit
CACCTGTACCCAGGTGAAATAAACAGCCATGTTGCTCACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GCCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCACATTATTTATAGAAAATGTACAGTTGTG20.1400560224089636No Hit
CCATTGCACTCCAGCCTGGGCAAAAAAGCGAAACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAACAACCC20.1400560224089636No Hit
GCCTGGCTTTGATTTGAAATTTTGTAAAAATTTCATGGCACCCAAGGTTTCTGATTCTGACCCAGCAGTGG20.1400560224089636No Hit
CCATTGCACTCCAGCCTGGGCAAAAAAGCGAAACTCCGTCTCAAAAAAAAAAAAAAAAAAAAACAACCAAA20.1400560224089636No Hit
CGTTGGTTGGCTGGATTCAGTAATAAATATGTAAGGCCTTTCTTTTTAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GATGCTATATTGTATTATGTAAATAATAAAAGCCATATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GCTCTGTATATTCTGTTAAAAAATTAAAGGACAGAAACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GCAGTGAAACGCTCCATGAATTGGTATAACAAGCCATAACACTAAATGAAATGATCAAAACCATAGGTAGC20.1400560224089636No Hit
GGTCACCAGCCAGGAGCCCTGACCCAGGCTGCCCAGCCTGTCCTTGTGTCGTCGTTTTAATTTTTCCTTAG20.1400560224089636No Hit
TTCCCTACACGACGCGGGCGGAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
CAATAAAGCACAGCTGGCTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
AGTTAAATAAATCATAAAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GGTCATTTAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GAATTAGTTACTGACTTCACCTGAGAAAAAAATAAATTCTCTATGGTGGTTTCAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GGGTTTGAGAGTGTTTGCCCAGCCCTTGTCTTGTGATGCTCTGGCTATAACATCCTTCTCATAGTCACCCA20.1400560224089636No Hit
GGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
ATATCAAACTACATTCTTCTGAAAGATGTTTCTATTATTTCTTAGGTCACTTCCATATATATTATATATAA20.1400560224089636No Hit
TTCCTGCTCTGCCCTCATTAAATTGCTTTTAATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
ACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1400560224089636No Hit
GTCTCCAGCCGTGCTTGGCCTCAGGGCTGGCAGCCGGATCCTGGGGCAACCCATCTGGTCTCTTGAATAAA20.1400560224089636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)