FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411
Sequences flagged as poor quality0
Sequence length71
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45532.246633593196314No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG251.771793054571226No Hit
GTTCAAAACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTGCTAAAATAAAATACAAAAATTAGCCAGGT201.4174344436569808No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT181.2756909992912826No Hit
CTTGGACCGGAACAAGGACCAGGAGGTGAACTTCCAGGAGTATGTCACCTTCCTGGGGGCCTTGGCTTTGA140.9922041105598866No Hit
GCCCGCCTACTTGCTGGTGTCCGCCGGCTGCTATGCCCTGGGCACTGTGGGCTATCGTGTGGCCACTTTTC110.7795889440113395No Hit
GTAATAGTTTGAATCATAGTGAACATCAATACTTGTTCCCTATATACGACACTTGATAATTAAGATGATCA100.7087172218284904No Hit
CTCTACCCAATCAGTTTCAAATTTAGACCTGACCACTGGTGCCACCTTACCCCAGTCAAGTGTAAACCAAG100.7087172218284904No Hit
GAGACTATCTGCACTACATCCGCAAGTACAACCGCTTCGAGAAGCGCCACAAGAACATGTCTGTACACCTG90.6378454996456413No Hit
GTTTTGAAAAGCTATTAGCCAGGATCATGGTGTAATAAGACATAACGTTTTTCCTTTAAAAAAATTTAAGT90.6378454996456413No Hit
GCTCAACGGGATCCTGCTGCACCTTGAGTCAGAGCTGACACAGACCCGGGCAGAGGGACAGCGCCAGGCCC90.6378454996456413No Hit
GTCTCCAGCATCTGTGGATCGCCAGAGGTCCCGGTCCCGATCAAGGTCCAGATCAGTTGACAGTGGCAATT80.5669737774627923No Hit
ATGCTCTGAAACTGTGGTTTGCTCCACAGATTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT80.5669737774627923No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC80.5669737774627923No Hit
GCTGCTTGGGAGGCTGAGGCAGGAGAATCACTTGAATCCAGGAGGTGGAGGTTGCAGTGAGCCAAGATTAT80.5669737774627923No Hit
GGATGCCAAATCTGTCAAGATCAAGAAAAATAAGGACAACGTGAAGTTTAAAGTTCGATGCAGCAGATACC80.5669737774627923No Hit
GGAGGGCGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAAAGGCATGAACCCAGGAGGCGGAGC70.4961020552799433No Hit
GGCTGAGGTGGGCGGATCACGAGGTCATGAGATGGAGACCATCCTGGTCAACATGGTGAAACCCCGTCTCT60.42523033309709424No Hit
GCTCCCACCGCAGTACCGCCTCCTGGAACGGAAGCATTTTCCTTTTTGTATAGGTTGAATTTTTGTTTTCT60.42523033309709424No Hit
GGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.42523033309709424No Hit
GCACGATTTCTCTTTTGATAATGCCCTTTAGGACACAACTAGTTATCAGTAACTGAATGTATCTTAATCAT60.42523033309709424No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA50.3543586109142452No Hit
ACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATAAGCTAGCAATCGCTTGAACCCCCGGAGGCGGA50.3543586109142452No Hit
GTCACAGGAAATGGACGCTGGATTACAAGCATGGCAGCTCAGGCAGCAGAAGTTGCAGGAAGAACAAAGGA50.3543586109142452No Hit
CCATGCTCTGGTGGCTGGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCATGGGCAAGAAGAAGATCG50.3543586109142452No Hit
GTGTGGTGGTGTGGGCCTGTAATCCCAGCTACTTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACCCGGGA50.3543586109142452No Hit
GGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACA50.3543586109142452No Hit
GGTATGGGGCTGTCTGGCAGGGTTTCCCAGAACCCTGTTTCCTGTGGTCATAAATATGGGTTTTCCTGACA50.3543586109142452No Hit
GTCATGTACCATCAATAAAGTACCCTGTGCTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.3543586109142452No Hit
CATTTAACACTCCCTCCCCCGCTTCCTCCTAGCCCTGTGGGCAGGGGTTGGAAACTTAGCACTTTATATTT50.3543586109142452No Hit
ACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAATCCAGGAGGCAGAGGTTGCAGTGAGCTGAGATCCCACC50.3543586109142452No Hit
GTTTTAGTGTGAGTTACCGTTACTGTATTTGTTTATTGTAAAGGTGGACATTTAGCGTTCAGTGCAGTTTT50.3543586109142452No Hit
CAAGTTCCACCACACTATTGGTGGCTCTCGCCGGGCAGCTTGGAGAAGGCGCAATACTCTCCAGCTCCACC40.28348688873139616No Hit
GAAACGGCGTCATGCCCAGCCACTTCAGCCGAGGCTCCAAGAGTGTGGCCCGCCGGGTCCTCCAAGCCCTG40.28348688873139616No Hit
GAGACACTGCAGGCGGTGCCCAAGCCAGGGGCCTTCGACCTGGAGCAGGTGAAGCGTTCCACCTACTTCTT40.28348688873139616No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.28348688873139616No Hit
CTGTTGGAATCAGCAGGCCTCTGTGCTCCCTCTGCCCTCCAGAACTCAGTGACTCTTGAACATGGATGTTT40.28348688873139616No Hit
CTGTTGGAATCAGCAGGCCTCTGTGCTCCCTCTGCCCTCCAGAACTCAGTGACTCTTGAACATGGATGTTA40.28348688873139616No Hit
GGTGGATGTTTACCGTGTGTTATATAACTTCCTGGCTCCTTCACTGAACATGCCTAGTCCAACATTTTTTC40.28348688873139616No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.28348688873139616No Hit
TCCTAGACCTAACCTGACTAGAAAAACTATTACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.28348688873139616No Hit
CTTAGACTTATACATCATTTTCCAACAGCTGGATGCATTCACAGTTTGGTGCATACCAATATGTATGAAAA30.21261516654854712No Hit
GGTGCAATCTCGGCAAACTGCAATGTCCACCTCCCAGTTCAAGCAATTTGCTTCAGCCTGGGCAACAGGGT30.21261516654854712No Hit
GCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGAGGTCCTCA30.21261516654854712No Hit
GCTAATAAAGGCGCACATGACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21261516654854712No Hit
AAATAAAACATTCTCCATCAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21261516654854712No Hit
GGTTTCAGGGTGCCAGTGGGAAGCCTGATGGGTGCTGGTGGCCTTTCCCCCGTGGATTGGTCTCTGGCCCA30.21261516654854712No Hit
GGCACCTCCAGCTGCCCCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCA30.21261516654854712No Hit
GTATTTATAGTCCATGTATAATAAATACATCTCTTAATCTCCTAATAAATTGGACCTTTAAACTACAAAAA30.21261516654854712No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA30.21261516654854712No Hit
GTTAAAAACAAGGATATCAGGAAATTTTTGGATGGTATCTATGTCTCTGAAAAAGGAACTGTTCAGCAGGC30.21261516654854712No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA30.21261516654854712No Hit
TTTTTAATTCTGTACTAAAATCTATAATAAAGCTTTTCCACAGCTTTAAAACTAAATAAAAAAAAAAAAAA30.21261516654854712No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.21261516654854712No Hit
CCCCTAGTGTGCCCAAGTCAGCCTCATATTCTGGGCGGACAGCTTGTCTGGACTTCGGAGTTGGGGGTGGT30.21261516654854712No Hit
ACAGTTTCCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
GGGTAAATGCAAAGTTCTGTGTTTAATTTCTTCAAAATGTATATATTTAGTGCTGCATCTTATAGCACTTT20.14174344436569808No Hit
GTAAAGCACTGTGGTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
TCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
CTTTTTACCCACCCATTCAATAAACCTTACAGAATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
GTGTAGAATAGGAAGTATGTGCCTGCGTTCAGGCGTTCTGGCTGGTTGCCTCATCGGGTGATGATAGCCAA20.14174344436569808No Hit
GATGAGGTATTAATCCCCTCCATTGCTCTCCAAAAGCCAATAAAGTGATTACACCCGAAAAAAAAAAAAAA20.14174344436569808No Hit
CACCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGAGAGGATCACTTGAGGTAGGAGTTTGAGACCAGCTG20.14174344436569808No Hit
GGAGAACTCACTGAGGATGAGGTGGAACGTGTGATCACCATTATGCAGAATCCACGCCAGTACAAGATCCC20.14174344436569808No Hit
GTGTTGAGTCATATACATTTACTGACCACTGTTGCTTGTTGCTCACTGTGCTGCTTTTCCATGAGCTCTTA20.14174344436569808No Hit
GGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAAC20.14174344436569808No Hit
CTTCAGTTCCCGGTAGGGCGAGTGCATCGCTTGCTGCGCAAAGGCAACTACGCGGAGCGAGTGGGGGCCGG20.14174344436569808No Hit
TTCCCTACACGACGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
TGCTGCTTGTGACGCTACTTACAATGAAATAGTCACATTTGAACGACTTCGGCCCGTCAATCAAAATAAAA20.14174344436569808No Hit
TCCCTACACGACGCGGGCATTAAATGCTAACTACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
CATGTGAAATAAAACTCAGTATTTTAATAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
GCCATGCTGTTCCGGCTCTCAGAGCACTCCTCACCAGAGGAGGAAGCCTCCCCCCACCCCTAATAAAAATT20.14174344436569808No Hit
ACAATCTACACATCTGACAAGGGACTAATATCCAGAATCTACAACAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
CTTCACTATTTTGCCCTATCCTTTGTGCTCATTCTTACTGAAATCTGTCTTGTCAGCTCAGGAATGGGATT20.14174344436569808No Hit
GCTTGGAAGTGAGCCCTGGCAGCCGGGAGACTTCAGCTGCCACACTGTCACCTGGGGCGAGCAGCCGTGGC20.14174344436569808No Hit
GCCTATAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGTAAGCGGAGGTTGCAGT20.14174344436569808No Hit
GCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGACAAATACACAGCGGTCCTCA20.14174344436569808No Hit
TTTTATTGGTGTTTGATTATTGGAATGGTGCCATATTGTCACTCCTTCTACTTGCTTTAAAAAGCAGAGTT20.14174344436569808No Hit
GGCTGAGGCAGGAGAATCGCTTGAACCCAGAAAGTGAAGGTTGCAGTGAGCCAGGATTGCACCACTGCACT20.14174344436569808No Hit
GTTGAAATGTGAATTAAGGAAAACTTTTGTTAATAAAAACTTTCTTTTAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
CCAGGGAGAAGGTTCTTCGCTCAGTATGAAGAAAAGCAACCCAAAACTCTCAATCTGATTTGTTTTTGTTT20.14174344436569808No Hit
TTCCCTACACGACGCGGGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.14174344436569808No Hit
ACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTT20.14174344436569808No Hit
GGCCCACAGCACCAATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAACCCCTGACC20.14174344436569808No Hit
ATTTGGGATTATTCCTCTGTTTGAAAAAAGAGGCTTGCTGTAATGTCACAGGAAACCTTTTAAAAGTGGAT20.14174344436569808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)