FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161
Sequences flagged as poor quality0
Sequence length71
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24120.757967269595177No Hit
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA242.0671834625323Illumina Single End PCR Primer 1 (100% over 58bp)
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAA131.119724375538329Illumina Single End PCR Primer 1 (98% over 58bp)
CCTAATATCAATTTGTAGGTAATGTTCCTGAAAATTGCAATACATTTCAATTATACTAAACATCACAAAGT110.9474590869939706No Hit
GGAATAATCTACCCTGACTAAAGCTTGAAATGCTACATTTCCAAGGTGAAGATGTGTGGGCACATGTTATG100.8613264427217916No Hit
GACTAAAGCTTGAAATGCTACATTTCCAAGGTGAAGATGTGTGGGCACATGTTATGGCAGATTGAAAAGGA70.6029285099052542No Hit
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTTAAA60.516795865633075Illumina Single End PCR Primer 1 (100% over 58bp)
ATTACACTGTACTACCCAAGAAGACTGTTTATTGTGAAGCATTTACCTTTCAAAAAATCATTACATTTCTA60.516795865633075No Hit
GTCGGGATCGTCGGTAAATACGGGACCCGCTATGGGGCCTCCCTCCGGAAAATGGTGAAGAAAATTGAAAT50.4306632213608958No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG40.34453057708871665No Hit
GGCGGCCTTTGTTGCTCAGCAAGGGGCTCTGCCCTCCCTCCTTCCTTCTTGCTTCTCATAGCCCCGGTGTG40.34453057708871665No Hit
CCCTACACGACGCGGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.34453057708871665No Hit
TGTTATGGCAGATTGAAAAGGATCTCATTCCATGGGAAAAAAAAAAATCCTGTCTTGTTCATAAATTGACA40.34453057708871665No Hit
GAGAAAGCCAAGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGT40.34453057708871665No Hit
GTGTTGCCCTTTGGAGCTTGGTGAGGAGGGTGGGCCTAGGGCTTGGCGAGTGCCACATCTGGCAGGTTTGG40.34453057708871665No Hit
TCTTCTCAGCCTCCCAGGTGCCTTGAGCCCTCTTGCAAGGGCGGCTGCTTCCTTGAGCCTAGTTTTTTTAC40.34453057708871665No Hit
GTTTTTTATGGTGACACAAATGTATATTTTGCTAACAGCAATTCCAGGCTCAGTATTGTGACCGCGGAGCC40.34453057708871665No Hit
TGCTTTGGGAGGCCGGGGCGGGCCGATCGCGGGGTCGGGAGATCGAGGCCGTCCTGGCTGGCACGGTGAAG40.34453057708871665No Hit
GGGTTACACTAATTGATGATTTAGAAATTAAAGCAGTCACTCCAGTGTGATGCAGGTGACTACTCTGAAAG40.34453057708871665No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATTCAGGTCGG40.34453057708871665No Hit
GCCGACGACAACACCACCATCCGTGTCACCAACTTGTCAGAGGACACGCGTGAGACCGACCTGCAGGAGCT40.34453057708871665No Hit
TTTCCAAGGTGAAGATGTGTGGGCACATGTTATGGCAGATTGAAAAGGATCTCATTCCATGGGAAAAAAAA40.34453057708871665No Hit
AAGCAAATGCTGAGAAGACTTCTGGCAGCAACGTAAAAATTGTGAAAGTAAAGAAGGAATAATCTACCCTG30.2583979328165375No Hit
TTCCCTACACGACGCGGGGGCGGCTGAACCGCCCGGCTGAGCCGACATTGCCGGCGTCTTGGCGATTCGGC30.2583979328165375No Hit
ACGCGGGGGAAACAAACACAAAATTCTGAATAAAATGACTTGGAAACTGAAAAAAAAAAAAAAAAAAAAAA30.2583979328165375No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC30.2583979328165375No Hit
GTGTATGTACATATATATATTTTTTAATTTGATGAAAGCTGATTACTGTCAATAAACAGCTTCATGCCTTT30.2583979328165375No Hit
GCCATTTATCCTGTGTTATGTGTTATATAATCTGAAATTTGTCACAATGTTACAATCAGGAAGTTTTTTTT30.2583979328165375No Hit
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCAGTAAAA30.2583979328165375Illumina Single End PCR Primer 1 (98% over 56bp)
GCATTTGGCCTCCTGCTTCCTTCTGTGGCCTCTGCCGGGCTCCATTCCCAAGGAAAAGAGAGGAGCTTGGG30.2583979328165375No Hit
TCTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGACACACGTATGCGAAGGGCCAG30.2583979328165375No Hit
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGATCTCGGTGGTCGCCGTATCATTAAAA30.2583979328165375Illumina Single End PCR Primer 1 (98% over 58bp)
GCTACTCAGGAGGCTGAGGCAGGAGAAATGCTTGAACTTGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGC30.2583979328165375No Hit
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATGATTAAAA30.2583979328165375Illumina Single End PCR Primer 1 (98% over 58bp)
GGTTAGCATTGCACAAAGCCCAGTTTCTTTCTGTGTTTGAAAAAGATTTTGATCCCCTTGGAATATTAAGA30.2583979328165375No Hit
GCTCCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAG30.2583979328165375No Hit
GGAATTTTATTTTGCTGAGTTGTTCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2583979328165375No Hit
CTTTCCCTACACGACGCGGGCCTCTGATTCTCTGTTGAAAAGTGTGTCCTTTCTCTCTGTCTGTACATGTT30.2583979328165375No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA20.17226528854435832No Hit
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAGTAAAA20.17226528854435832Illumina Single End PCR Primer 1 (98% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.17226528854435832No Hit
GTCTTAATTTGTAAAAAATAAAGAAAATTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
ATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCTCATATCCTCCCTACTATG20.17226528854435832No Hit
AAATAAAGCTTTTTGATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
GCCTGATGTCTTAACGAATAAAGGTCCCATGCTCCACCCGAGGACAGTTAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG20.17226528854435832No Hit
ACGCGGGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
GGAGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGC20.17226528854435832No Hit
AGTTATAACTGTCATACATTTGGAATATTATGATCCCAAGTAGTCTTTTTATAATTTGGATTGTATCGTAT20.17226528854435832No Hit
TTGGGGACTACTAGATGGAAGAGAGAGGGAGTGAGTCAAGGGCTGAAAACCTACCTTCTGGGTACAATGCT20.17226528854435832No Hit
GCCTGCTGCATAAACTCTTAAATTTGATTATTCCATAAAGGTCAAATCATTTTGGACAGCTTCTTTTGAAT20.17226528854435832No Hit
CCCCTGGCAGCAGGGAAGGGCTTTGCCTGACAACACCCAGCTTTATGTAAATATTCTGCAGTTGTTACTTA20.17226528854435832No Hit
GTTCAGCAGGCTGATGAATAAGATCTAAGAGTTACCTGGCTACAGAAAGAAGATGCCAGATGACACTTAAG20.17226528854435832No Hit
TTCCCTACACGACGCGGGCTGTCTTGATATAAAGGAAAAGCAAAATCTGTATAACATTATTACTACTTGAA20.17226528854435832No Hit
GCTGCTGAGGCCTGTGGGTGGGACACCCAGTGCGAAACCCTCATCCAGTTTTCTCTCCATCTCTTTTCTTT20.17226528854435832No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
GAGTCCATCTGTTTGTCCATCTGCTGAAATGAGAAAAGAAAAATTTATGCACTGATTTAAAACAAACCGAA20.17226528854435832No Hit
CTGGCCAACATGGCGAAACCCTGTGTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGTGCATGCCTG20.17226528854435832No Hit
GCCGCTTGGGCGGGCGGCCAGTGGCGACTACAGCAGGGACTGGCTGCCAACCCCTCCGGCTACGGGCCCCT20.17226528854435832No Hit
CATCTCACCTCTCGCAAGAGGGTCTCTTTGTTCAATTTTTTTTAATCTAAAATGATTGTGCCTCTGCCCAA20.17226528854435832No Hit
ATATGGTTCAAATGTAACAGTGCAGAATTGAATATGGAGGCATGCATAACCTTCCTCTTAGAAAATGGCAG20.17226528854435832No Hit
GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGGCC20.17226528854435832No Hit
GGAGAGATCGTGCTGTGTGTCATGTCTGTTGTTCAAGTAAATAAAAGTTGCCCTGACTTCAAAAAAAAAAA20.17226528854435832No Hit
CTGTTCAAAAATAACTCAGACCCAGTCTTGTGGATGGAAATGTAGTGCTCGAGTCACATTCTGCTTAAAGT20.17226528854435832No Hit
CAGTTCCAACTGTACATACATACTGTATGAAGCTTGTCCTCTGACTAGGTTTCTAATTTCTATGTGGAATT20.17226528854435832No Hit
GTGTCAAGTGATTAAGTGTGTATTTGTACCCTAGATGATATGAACCAGCAGTCTTGTTTTGGCATCATCCT20.17226528854435832No Hit
CCCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
GTGGGTGAATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAAAATGATGATACCCTGCCTCTACTAA20.17226528854435832No Hit
CTATTTTACTTTGTACTTGTGTTTGCTTAAACAAAGTGACTGTTTGGCTTATAAACACATTGAATGCGCTT20.17226528854435832No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
TTCTTATACAAGTCAACAGTCTAGCAGATCAGCCAGTCTCTTTCATCATCAAGCAGACATACTCAAGGTAC20.17226528854435832No Hit
CTCTTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTGTCATTAAAA20.17226528854435832Illumina Single End PCR Primer 1 (98% over 58bp)
CTACTCGGGAGGCTGAGGCAGGAGAATGCTGTGAAACCAGGAGGCAGAGCTTGCAGTGAGCTGAGATCGCA20.17226528854435832No Hit
GGTAGCTGACCCTCGTCGCTGCGAGTCCAAAAAGTTTGGAGGCCCTGGTGCCCGCGCTCGCTACCAGAAAT20.17226528854435832No Hit
AAAATTGTATTACTTCTGTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit
ATGCGGAAGAGTCTTCCTCGGAGCCTGAGGAGGAAAATGTGGGAAACGACACCAAAGCCGTTATTAAAGCA20.17226528854435832No Hit
GGCCAAATTCTTCAGCAGAAGAGCTGAGGAGAAGATTAAGAGTGTTGGGGGGGCCTGTGTCCTGATGGCTT20.17226528854435832No Hit
CGTTGTTTATTCTGCGTCCCCTTCTCCAATAAAACAAGCCAGTTGGGCGTGGTTATAAAAAAAAAAAAAAA20.17226528854435832No Hit
CTACCATAGTGAGCCATGATTTTCTAAAAAAAAAAATAAATGTTTTGGGGGTGTTCTGTTTAAAAAAAAAA20.17226528854435832No Hit
GGTGAAAACACTGTAGTAATAAATTTTCATATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.17226528854435832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)