FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14882
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3912.627335035613493No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA710.4770864131165166No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC610.4098911436634861No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.2889396586480312No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390.262061550866819No Hit
CTCTTATTGTACGCTTTATTATAAGTTTAGAAGGCAGTTTATTCTAAATAATTTTTCTCTAGGAAGGCGTA340.22846391614030373No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT330.2217443891950007No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG280.18814675446848542No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT260.17470770057787932No Hit
GTATAATGTTCTGTTTAAAATAATTTCTAGGATTTTTTTTCCTTTTATATCTGTAAGTAAAAAATGTATAA260.17470770057787932No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC250.1679881736325763No Hit
GTGAACACACTGATTAGGTTATGGTTTAATGTTACAACAACTATTTTTTAAGAAAAACAAGTTTTAGAAAT250.1679881736325763No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC230.15454911974197016No Hit
CTACCATAGTGAGCCATGATTTTCTAAAAAAAAAAATAAATGTTTTGGGGGTGTTCTGTTTTCTCCAAAAA230.15454911974197016No Hit
TTCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.15454911974197016No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.15454911974197016No Hit
GGGCATGGTGGCACATGCCTGTAGTCCCAGCTACTCCAGAGGCTGAGATGGATCATCTGAGCCTCAGGAGG220.1478295927966671No Hit
CTTCTGGGGCCTTCGTGTCCGAGGCCAGCACACCAAGACCACTGGCCGCCGTGGCCGCACCGTGGGTGTGT210.14111006585136407No Hit
TTCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.134390538906061No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA190.12767101196075797No Hit
GGCGTGGTGGTGGGCGCCTGTAGTCCCAACTACTCGGGAGGCTGAGACAGGAGAATGGCGTGAACCCAGAG180.1209514850154549No Hit
CTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC170.11423195807015186No Hit
GTTTAGTTCTTATATTTGTTAACTGATTTCGGTGTCTTGAATATATTTTTTTAAATTATGTGTATGAACAA170.11423195807015186No Hit
GGTTTCAAGTGTACATGTGTGAAAACAATATTGTATACTACCATAGTGAGCCATGATTTTCTAAAAAAAAA170.11423195807015186No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11423195807015186No Hit
ATTTAAGTGTATTTGCTATTGTTTTGAAAGTGCTGACAAAGTTTATATTTGTAACATCAGCCTTCCTCGCC160.10751243112484882No Hit
GTTCAGAACTGCGGATTATTCCTAGAGATCGTGTTGGAAGTTGTTAATATCTGCTACTTGGAACATACGAT160.10751243112484882No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC160.10751243112484882No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10751243112484882No Hit
ATGTGAATATGTGTAAAAGTGCTTTTCAGAACTGCATTACTATGAGAAAACATCAAAACTGCTGTAGTTTT150.10079290417954576No Hit
CGCTTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAA150.10079290417954576Illumina Single End PCR Primer 1 (100% over 58bp)
CCTCTGACCCACAGCAGAGCAAGTGCTGAGATCATAGGCTCTGTTAGCAGCTAGAGAGGATTTTTTTTTTT150.10079290417954576No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10079290417954576No Hit
GTGCTGATGAGCCCATGACTACCTTTGTCTTATGCAATGAATGTGGCAATCGCTGGAAGTTCTGCTGATGG150.10079290417954576No Hit
ATACTGGAGGGATTTCATTTTTAATTCATCTTTATGAAGATTTAGAACTCATTCCTTGTGTTTAAAGGGAA150.10079290417954576No Hit
GAGTAGGGCCGCCTCGGGGCTGGGCATCCGGCCCCTGGGGCCACCCCTTGTCAGCCGGGTGGGTAGGAACC150.10079290417954576No Hit
GTTACATCTCATTCAATCTCCAGAAGATTGGGATTATCGTCTTCTAAGAGGTTGCTAATGCCTTTCATCTT150.10079290417954576No Hit
GGATATAGTGTACACTGCTTGGGTGATGGGTGCACCAAAATCTCAGAAATCACCACTGAAGAAGTTATCCA150.10079290417954576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)