FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683
Sequences flagged as poor quality0
Sequence length66
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41824.836601307189543No Hit
TTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCAT311.8419489007724301No Hit
GGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCC90.53475935828877No Hit
CTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGAT90.53475935828877No Hit
ATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTA80.4753416518122401No Hit
GTTCACCCCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATC70.41592394533571003No Hit
CCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCG60.35650623885918004No Hit
CCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATC60.35650623885918004No Hit
CCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTAC60.35650623885918004No Hit
GTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCC60.35650623885918004No Hit
ATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATAC50.29708853238265004No Hit
GGCATCATACCTACCTCACCGACGCCCTACACTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGG50.29708853238265004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.29708853238265004No Hit
GGGCACGTCCGTGGTGCTAATCTGCCCGCAGGACGGCATGGAGGCCATCCCCAACCCCTTCATCCA50.29708853238265004No Hit
TTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAACTCTGGCATCAT50.29708853238265004No Hit
GACTTGATGACACCCCAGTTCACCCCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGG40.23767082590612004No Hit
TAACTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTT40.23767082590612004No Hit
CAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATTATCTATGTGATGCTGTGCGGATACCCTCC40.23767082590612004No Hit
GTTGAAGACTTCCATCAACTAGAACCACGAGGGCTGATGGACAGACCACAAGGCCAGTTTGTTCCA40.23767082590612004No Hit
GGATAACTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGC30.17825311942959002No Hit
CCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAACTCTGGCATC30.17825311942959002No Hit
CCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGATGACACCCCAGT30.17825311942959002No Hit
ATCTCATACTTGTATAGAGTAATAACAACAGTTGTACTTCCACCAAAGGCATGTGGCATATTTACC30.17825311942959002No Hit
ATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAACTCTGGCATCATACCTG30.17825311942959002No Hit
GGATCTTTGACAGGGATCCAGACACACTACTTTATTTACTTCAGCAAAAGAGTGAGCCAGCAGAGC30.17825311942959002No Hit
CTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCA30.17825311942959002No Hit
ATGCAGAATCCACGCCAGTACAAGATCCCAGACTGGTTCTTGAACAGACAGAAGGATGTAAAGGAT30.17825311942959002No Hit
CCCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACC30.17825311942959002No Hit
TTATTATGTAGCGCCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCAT30.17825311942959002No Hit
AAATGTGTCTGTATGCAATAGCTAGAAAAATGCCTGTGTCTTAAGTCATTACTCATGTTCTAATTT30.17825311942959002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.17825311942959002No Hit
ATACCTACCTCACCGACGCCCTACACTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATT30.17825311942959002No Hit
CTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATTATCTATGTGATGCTGTGCGGATACC30.17825311942959002No Hit
CAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTACAAC30.17825311942959002No Hit
CTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.17825311942959002No Hit
CTCTTTGGCTGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGAT30.17825311942959002No Hit
ATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTC30.17825311942959002No Hit
GAGAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTACAACAAGAGCTGTGACTTGTGG20.11883541295306002No Hit
CAATAAACTGGTATGAATAATTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
GTACCTACCTCACCGACGCCCTACACTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATT20.11883541295306002No Hit
ACTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGG20.11883541295306002No Hit
GGAGAGAGAGGCTGTAGTGAGCTGTGATAGCACCACTGCACCTCCAGCCTGGGCAACAAAGCAAGA20.11883541295306002No Hit
GGAGAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTACAACAAGAGCTGTGACTTGTG20.11883541295306002No Hit
GTGATTATCTATGTGATGCTGTGCGGATACCCACCTTTTTTACTCCAAACACCACAGCCGGACTAT20.11883541295306002No Hit
GGGCTGCACTGCCACCCTGGGCAACTTCGCCAAGGCCACCTTTGATGCCATTTCTAAGACCTACAG20.11883541295306002No Hit
GTTCTGAAAATAAAAAACTTGAGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
ATATACACTCTCAGGTATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
GCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGCGGGCACC20.11883541295306002No Hit
AACCTGGCAGGCAGAGGTTGCAGTGAGCTGAGTTTGAGCCACTGCACTCCAGCCTGGGCAACAGAG20.11883541295306002No Hit
GGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGATGACACC20.11883541295306002No Hit
GGAGGTCAGGGCTTCAGTGAGCCGTGATTGTACCACTGCATTCCAGCTTGGTTGAGAACCTATCTC20.11883541295306002No Hit
TTACAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCTTTGGCTCTGCGGCACTGTCACTTGT20.11883541295306002No Hit
ACTTCTAAGTCTGAATCCAGTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCT20.11883541295306002No Hit
AGCCTGGACCGCTCCAAGGACAAGTGAAGGTCAGCCGGCCGGGCGGGTCCACAGTTACCAGCACGC20.11883541295306002No Hit
GCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTAC20.11883541295306002No Hit
GTTCGCCCCTTATTATGTAGCGCCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAGTC20.11883541295306002No Hit
CCCCAGGTACTGGAGGCGCTAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCG20.11883541295306002No Hit
GATGAAAATAGTGACCAGAGTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
ATAATAAACTGAGTCATTCTCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
AATCAATTTATCACTATTTAAAACTTTAAATATCTACAATCTTTACCAAAATACTTTTTTATTTAA20.11883541295306002No Hit
GTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGCCGCCC20.11883541295306002No Hit
AGGTGACTTGATGACACCCCAGTTCACCCCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAG20.11883541295306002No Hit
CCCCTGGATAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
TCCCTACACGACGCGGGAATATATAAATACTCAAGTCCAATATTAAAAACTTAGGCACTTGACTAA20.11883541295306002No Hit
GGATTTAGTCCTGGCCCAGCCCAGAGCTGGGACCTGGAGCACGATCTGTTGACTTCCCTGGGTAGG20.11883541295306002No Hit
GTTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATGC20.11883541295306002No Hit
CTTCGGAACATTTGATCATAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
GGTGGGTAGAGACACTTAATAGTATCATGTCGCATGCAGATGTCACATCGGCCTCTGCAAAAACTG20.11883541295306002No Hit
CAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGATGACACCCCAGTTCC20.11883541295306002No Hit
CCTCAAGCCTGAAATCTGCTTTTTAAGGATAACTCTTTGGCTGCCCCAGTGAAGTTGTGTGACTTT20.11883541295306002No Hit
AAGGATAACTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGT20.11883541295306002No Hit
GGGCGACTCTAGTGGCCTGAGGAGATGTATTTATAGGCCCCCAGCAGGGCTGCTCCCCCCTCGGCC20.11883541295306002No Hit
GTGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTC20.11883541295306002No Hit
GTCCAAGTACATCGACAAGACGATCCGGGTAAAGTTCCAGGGAGGCCGCGAAGCCAGTGGAATCCT20.11883541295306002No Hit
GGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCCTACACTTACAACAAGAGCT20.11883541295306002No Hit
GGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGCCGCC20.11883541295306002No Hit
GATGCCTTTTCCATCCTGGACTTATTCCTGGGACGTTGGTTCCGGTCCCGGTAGCCTTGTTAACCC20.11883541295306002No Hit
CCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCGCCG20.11883541295306002No Hit
GCACTTTCAGGGACTTCCCTCCAGCTGCCTCTTCATCTGGGAATGAGCTAAGCAAGGCTGAGCCTC20.11883541295306002No Hit
CCCCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCA20.11883541295306002No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11883541295306002No Hit
GTACTGGAGGCGCGAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTACCTCACCGACGCCC20.11883541295306002No Hit
CTCTTTGGATGCCCCAGTGAAGTTGTGTGACTTTGGATTTGCCAAGATTGACCAAGGTGACTTGCT20.11883541295306002No Hit
GGCAGAAACGGAGGCGCGCTTTGGAGCCCAGCTGGCGCATATCCAGGCGCTGATCAGCGGTATTGA20.11883541295306002No Hit
ATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAATCTGGCATCATACCTG20.11883541295306002No Hit
TTATTATGTAGCACCCCAGGTACTGGAGGCGCTAAGAAGGCATCAGAAGGAGAAATCTGGCATCAT20.11883541295306002No Hit
CCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAACTCTGGCATCATACCTACCTCACCG20.11883541295306002No Hit
CAGTTCACCCCTTATTATGTAGCACCCCAGGTACTGGAGGCGCAAAGAAGGCATCAGAAGGAGAAG20.11883541295306002No Hit
GAATCAAACAGATGTTGCTCTGGCAAACCATCCTCCACCCTACCGACTAGATATTGCTACAGAAAT20.11883541295306002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)