FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20837
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5192.4907616259538323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250.5998944185823295No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA590.28315016557085954No Hit
TTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAA420.2015645246436627No Hit
GCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATA420.2015645246436627No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG400.19196621394634544No Hit
GTGCAGCAATAACTGCACTGTCTAAAAGTTTGTGCTTATTTTCTTGTAAATTTGAATATTGCATATTGAAA340.1631712818543936No Hit
ATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAAGAGAATT320.15357297115707635No Hit
GTGTTCACACCACAAAATATAACTATGTGAGGTAATGCATGTGTTAATTAGCTAGATTTAACCATTGCATA310.14877381580841773No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA280.1343763497624418No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACC280.1343763497624418No Hit
ACCAAAATACTTCCATATCAATTATTTGAACTCGGTAGTTGTTTGACCTAGTTAGATGTGGTGTTTATTCA280.1343763497624418No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAA270.12957719441378318No Hit
GCTCAATGTCCCTCTGTGCTTTCACGATGCTGATGGATTATGAAGGTAGGGAAGAATGTACAGCCAGGAGG270.12957719441378318No Hit
CTTTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCA270.12957719441378318No Hit
CTTTTGTATAGGGTATCGTGTTAATTTTTACATGCACCCAGAGCAGTCTGCCTTTTGGAAGATACATGGGA260.12477803906512452No Hit
GTATTTAGGTGCTGGGAAGACAACACTTCTGAACTATATTTTGACAGAGCAACATAGTAAAAGAGTAGCGG260.12477803906512452No Hit
GCCCTGGAGGGGCTGAAAATGGTGGAAAGGACCAAGATGGCGGCCGCAAACTGACACCTCAGGGACAAAGA250.1199788837164659No Hit
GTCTAGGGAACTTATAGAAATGCTGGCAATTTCAAGAAACCAAAAGTTGTTACAGGCTGGAGAGGAAAACC250.1199788837164659No Hit
TTCCAATCCCCTCCCTGTGAATGTGTGGAGAAAAAGAGATGGGAACGAGGCAGAGGAAATAGAGAAATTTT250.1199788837164659No Hit
CATTTGGATTTTTTTAAAACAAGTAAATTTCTTATTGATGGCAACTAAATGGTGTTTGTAGCATTTTTATC250.1199788837164659No Hit
CATATATGCGAATCTATAAGAAAGGTGATATTGTAGACATCAAGGGAATGGGTACTGTTCAAAAAGGAATG240.11517972836780728No Hit
CCACTGTCCTCTGCCAGCCTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAGCAG240.11517972836780728No Hit
ATGATACATAATCTACAATAAGGGTCTTAGTGCTTTAGAAAAAAGTTAAAATTGGAAGTCATTAAAAAAAG240.11517972836780728No Hit
ATTTTAATCTCAGCACTTTGGGAGCCCAAGGTGAGCAGATTACTTGAGGTCAGGAGTTGGAGACCAACCTG240.11517972836780728No Hit
AGAAAGGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT230.11038057301914862No Hit
GGCAACAAATTCTTCATTTTACCTAGGGAAATATGTAGAGCTAGAGACAGGAAGAAACTTGTCTAGTGTCA230.11038057301914862No Hit
GAGTTAGCTTGGTCATTTAATTGCGACTTCATGTTATTTGATTGAAATAACCCACATCCTTGCTTTGTATA230.11038057301914862No Hit
CACAGGCACAAGAATCTAGTAAATTCTAGGTTTCTTGTGTGGAAATCAGTGTGCAAAATCTTAACTGAGTG230.11038057301914862No Hit
ACCCAAACATTGCCAAGAGAAAATGCAATGTGAGGTAAAAGATGAAAAAGATGACATGAAAATGGAGACTG230.11038057301914862No Hit
GGGGTGCCTCATGTAGTAGGTGACACCTAAACTACTGTTTGAAAGCCAGACAACTAAAGGAGTTAGAGAAA230.11038057301914862No Hit
GGCTGCTGCCAACCCACCTGGAGGCCACCTCCCTCCAGGAGATCCCAGGGTGCAAAGTGGCATTGAGACAG230.11038057301914862No Hit
GGGTGATACAGATGGTGATTTTAAAGAGCAAAACTAGACTTCTATGTGAGAAGTGCTGGAAAATGATTTAG230.11038057301914862No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCT230.11038057301914862No Hit
CTTCTAAGCTACTGCTTCTCCACTGTCATGTATAAAGCTCTTCCTTTGAAGCCTAAGCTGTCTTGCAGTTT220.10558141767049001No Hit
GAAGAGCATAGAATAAAATCTGAAAGTACAAACCATGAGCAACAATCTCCTCAGAGTGGAAAAGTTATTCC220.10558141767049001No Hit
GTCTACCTTTCTTCAGGGTGAAATTAGTAAGTAAAAGGCTGCTGCAGCACCGAGAAAGGACCGATGCAGGA220.10558141767049001No Hit
TCATGAGCCTCCTCAACAAGCCCAAGAGTGAGATGACCCCAGAGGAGCTGCAGAAGCGAGAGGAGGAGGAA220.10558141767049001No Hit
ATTTAAAAACTTACTGTGATAAGATTATTTATTTACTTATTTGAAAAGTTTGTTAAAAATACTTGCTTATA220.10558141767049001No Hit
CGGTTAAAATCAGAACGAGCTGAAGCCTTAAAGCAGAGACCTGATGGGCGATCTGTTTCTGAGTCACTAAG210.10078226232183135No Hit
GTCTCTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAACCTACAGGAGG210.10078226232183135No Hit
GAGAATTAATGTGCGTATTGAGCACATTAAGCACTCTAAGAGCCGAGATAGCTTCCTGAAACGTGTGAAGG210.10078226232183135No Hit
TGATTTATGTTTGATAATTAATGACAAAAATGATGCTTTCAACATATTTCACTTATAAATTATATCCCCTA210.10078226232183135No Hit
GTCGACCTGTGGCAAATGTGGCTACCCTGCCAAGCGCAAGAGAAAGTATAACTGGAGTGCCAAGGCTAAAA210.10078226232183135No Hit
GTTCAATATACGCAAATCAATAAATGTAATCCAGCATATAAACAAAGCCAAAGACAAAAACCACATGATTA210.10078226232183135No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAG210.10078226232183135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)