FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20638
Sequences flagged as poor quality0
Sequence length71
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12636.119779048357399No Hit
TCCCTACACGACGCGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.44093419905029557No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG650.3149529993216397No Hit
GATCGGAAGAGCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.18897179959298382No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.17443550731660046No Hit
CCATCATCCCGCCTCTGTTTACAGTCAGCGTGGACCATCGGGGCACTTGGAACGGGCCCTGGGTGTCCACT340.16474464579901155No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.1598992150402171No Hit
AGATCCTTCCAGCGACTGGGAAATGTCACAGTCTTCAATAAAGAGTTCTATCTGTCTTCTACGCGGGAAAA320.15505378428142264No Hit
AGCCTCTCAGCTTTTTCGTATTTGGAGATATAACCAAGAACTAAAAGCTAAAGCACACAAATAAAAGAGTT310.15020835352262818No Hit
TCCCTACACGACGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.15020835352262818No Hit
GCCCCAGGCTCTGCAGCCCCTGCTGCTGGTTCTGCCCCTGCTGCAGCAGAGGAGAAGAAAGATGAGAAGAA310.15020835352262818No Hit
TAGGAATATTGACTTACAAGATCAGGAAAAACATTTAAGTCAAGAGGATAATGATCTCAACAAACAAACTG300.14536292276383372No Hit
ACCCTGGAGGAGAAGAGGAAAGAGAAAGCCAAGATCCACTACCGGAAGAAGAAACAGCTCATGAGGCTACG300.14536292276383372No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCACTATTAAAGGTTCGTTTGTTCAA300.14536292276383372No Hit
ACATAATATCAGATATTACGGATGTTAGATTGCATCTCAGTGTTAAATCTTTACTGATAGATGTACTTAAG280.1356720612462448No Hit
GATCGGAAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.12598119972865587No Hit
TAACACCACCAGTGGTCTTAAGGTTGAAGTGTGGTTCAGGGCCAGTGCATATTAGTGGACAGCACTTAGTA250.12113576896986142No Hit
GATCGGAAGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11629033821106696No Hit
TTCCCTACACGACGCGGGGAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11629033821106696No Hit
GTGCTGAATGGCTGAGGACCTTCCCAGTCTCCCCAGAGTCCGTGCCTTTCCCTGTGTGAATTTTGTATCTA240.11629033821106696No Hit
TGTCTGGACGTGGCAAGCAGGGCGGCAAGGCTCGCGCCAAGGCCAAAACCCGCTCCTCTAGAGCTGGGCTC240.11629033821106696No Hit
GCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11144490745227251No Hit
TCCCTTGCCGACCCAACGGCAAACCAACTGCTTGTTTAAGGAAATCAGTGGAATGTATGCAATTAATTACA220.10659947669347805No Hit
GGTGGAGAGCTCTATGAAGAGTGGCTGGAACTTAGAAACGGTTGCCTCTGCTGTTCAGTGAAGTGAGGAAT220.10659947669347805No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG220.10659947669347805No Hit
ATCTTGGGGCCTGTTTCCCATGGGATTCAAGAGGGACAGCCCCAGCTTTGTGTGTGTTTAAGCTTAGGAAT220.10659947669347805No Hit
GCCCTCAAGGTCGTGCGTCTGAAGCCTACAAGAAAGTTTGCCTATCTGGGGCGCCTGGCTCACGAGGTTGG220.10659947669347805No Hit
ATAAGTGCTGTCAGGCATAATGCATTACGGATGGACTTTGACTAAGGTGATGTAGAGTTGCCATAATCAGA220.10659947669347805No Hit
ATGAAGTATTTGGACTATTTCAGAAACTGGAAAATATCAATGGTGTTACAGATGGCTACTTAAATAGCTTA220.10659947669347805No Hit
TCCCTACACGACGCGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1017540459346836No Hit
GAATCCTTTGCAGCTACAGCTGACTTCAGGGAGCAGATTTTAAGAGTAAAAGAAGATGAGAATGTTCCATT210.1017540459346836No Hit
CCCAACATAGAGTCTTGTGGCTGGTCAGGCACAGTGGCTCACCTGTAATCCCAGTACTTTGGGAGGCCAAA210.1017540459346836No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT210.1017540459346836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)