FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3293
Sequences flagged as poor quality0
Sequence length71
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50815.426662617673854No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG320.9717582751290618No Hit
CATTTACCTACATAACAAACCTGCAGGTCCCGCACATGTATCCCAGAACTTAAAATAAAAATAAAAATTAA270.8199210446401458No Hit
CAGTGACAAAAGGCTCGGAATGTTACAATGGTAAAATGAGTGATCTCAAATCCACTGACAGATATAAAATA240.7288187063467962No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180.5466140297600972No Hit
CTCCATCTCAAAAAAAAACCAAAAAAACAAATGTTAGGAACCTGAATATCTAAATCAAGCTTTAAAGAAGC170.516246583662314No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG160.4858791375645309No Hit
GCTCCTAATATGGCAGTTGTGAATGAACAGCAAATGCCAGAAGAAGTTCCAGCCCCAGCTCCTGCTCAGGA140.4251442453689645No Hit
GCCTGTGGGACCAAAGCAGTATCCTTACAATAATCTGTACCTGGAACGAGGCGGTGATCCCTCCAAAGAAC130.39477679927118126No Hit
CCGACAGTGGTACGAGTCCCACTATGCGCTGCCCCTGGGCCGCAAGAAGGGAGCCAAGCTGACTCCTGAGG130.39477679927118126No Hit
GTTTAAGATCTGGCATTCAGTTGTTACACATGTGGTTCAATGATTTATCATGAACCCTAAACTGCACACTG130.39477679927118126No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT110.33404190707561493No Hit
GGAGTTCAAGGCTGTATTGTAGTGAACTAAGATCATGCCACTGCACTCCAGCCTGGGCTACAGAGCTAGAC110.33404190707561493No Hit
GGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAA110.33404190707561493No Hit
GAAGTACTTCTTGCATGACGACAGAGATGATGGTGTGGATTATTGGGCCAAAAGAGGAAGAGGTCGTGGTA110.33404190707561493No Hit
GGACTTGAACTCAGCTCTAGACCAGGTGGACCTGATAGATATCTACAGAACTCTCCCCCCCAAAACAACAG110.33404190707561493No Hit
GGGATGATAAAGATGAGGTTTGCGGCTGTGGCCCGCTGGTGGGCTGAGCCACAAGGCCCCCGATGGCCCAG110.33404190707561493No Hit
GAAGCCCAGATGGCTCAGTGAATCCTTGCAGAACTTCTGTGCACATGACATCTTTTTCCATGTTGTGCAGA100.3036744609778318No Hit
GATTTTAACAAATCCTATTAGATTTGTATCAACTAGTTACATGTTCTATTCATAGTCTTTTGTGAATCATT100.3036744609778318No Hit
AACTGGATCCTGGCAGCGAGGAGACCCAGACGCTGGTTCGAGAGTACTTTTCCTGGGAGGGGGCCTTCCAG90.2733070148800486No Hit
GTGCAGGATGAAGGTGGGGGTGGGCTAAAGCCATAGGAAGAAAAATGTGATGTATGTGTCCAGTATGTACT90.2733070148800486No Hit
GAATAATGGTGAACGAGTGTTATGTATTTTGATAAAGTAAAAATAATGCACTAAAATAATGGAAAAAAGCA90.2733070148800486No Hit
GCCTCTGAGCTACCTCGACTTTGGACAGAGTAATGACTCCCCAGAAAACGTCATATAAGACTTTACCAGCC90.2733070148800486No Hit
CTCTACTAAAAATACAAAAATTAGTCAGGCATGGTGGTGCACGTCTGTAATACCAGCTTCTCAGGAGGCTG90.2733070148800486No Hit
GAAGTAATCATACTTCTCATGTCTCCGAACAGAGAAGTATGATTACTTCAGTGTTAGTTTTCTTAATTTTT90.2733070148800486No Hit
GTACAGGAATTTGCAAAGAGATGTAAACATACATTAATTTCCAAGTACATTTCTCCCCTTCTCCCTGGAGG90.2733070148800486No Hit
GCAATAGAGGAAGGAAATGCAGAAAATTATAAGGCTGAAATAGAAACTGGCCACAGCAGAGATTAAAGAAC90.2733070148800486No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA80.24293956878226544No Hit
CTCAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAA80.24293956878226544Illumina Single End PCR Primer 1 (100% over 58bp)
GTGGGGGAGGCATTAGCATATCAATTTCCAATGAGCACAAAATTCAAAATACATTAAAAAGTAGCATGGCT80.24293956878226544No Hit
CCTTCTGAGGCAACAGCTCAACTTCCTGTGGTCTGCCCTCCCCACAGCTCTGTGATCAGCAGCTGCCCGGG80.24293956878226544No Hit
GGGTGAGTAAGTAGTTGGGTATAAGATCTGAACTTTTCATCTGCAGAGGCAAGAAAAATATTTGACATTGT80.24293956878226544No Hit
CATCTATCATCAAGAGATTCACAATCAAGATCTGTATCAAGATAAAGGAATTCAAATAGCATATATATGAC80.24293956878226544No Hit
GCGCGACAAGATGGCGGATAAGGAGAAGAAGAAAAAGGAGAGCATCGTGGACTTGTCCAAGTACATCGACA80.24293956878226544No Hit
GCTCAGGTCTGCTTGGAGGATGCCTCCCCCAGGAGGGCAGTGAGGGATGCCGCAACCTCGACTTCTCAGCC80.24293956878226544No Hit
ATTGTTACACTTTATTCAGATTACAATTAATTAGAGTGATTATGAATTAATTAGTGTTCTACACCATTACT80.24293956878226544No Hit
GCTATCGGTGCTTTGCAGGAGGCAAGTGAGGCCTATCTGGTTGGCCTTTTTGAAGACACCAACCTGTGTGC80.24293956878226544No Hit
GGGAGGGAGGTGTTGCCGTCACTGTATTAAGTCGATGTTGGGAAACGTTTTAACATCTGGAGCCTTTGTGG80.24293956878226544No Hit
GGAAGAAGGTGGGGAGGAAGAGGAGGAGGAAGAAGAAGGTGATGGTGAGGAAGAGGATGGAGATGAAGATG80.24293956878226544No Hit
GGTACTCGTACACTATTTTTTTGTACTGCTGGTCCTGTACCAGAAACATTTTCTTTTATTGTTACTTGCTT80.24293956878226544No Hit
GTTGCTCAGGAGTGACAAGTTGCTAATATGCAGAAGGGATGGGTGATACTTCTTGCTTCTCATGATGCATG80.24293956878226544No Hit
AGGTTTCCACAGCATCCCCCTTGAGTGATATGTTTCCATTTCTCCGCTTTTTATAGTTAAGGCATTTTTTT70.21257212268448225No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAAT70.21257212268448225No Hit
GATGTTTGTCTTTTTCTTACTAATTTGTAAGAATTCTTGATGGGTTTCTGTTAGTCATGTATTTTGCTAAT70.21257212268448225No Hit
GACCTCGTGATGTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGTTGTGAGTCACCGCGCCCGGCCA70.21257212268448225No Hit
ATTTTGGGTGATACTTTCATTTTGCACATCATGCACATCATGGTATTCAGGGGCTAGAGTGATTTTTTTCC70.21257212268448225No Hit
CCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAG70.21257212268448225No Hit
GATGGATGGACCACAGTACGACGTTAAGTCTCAAGATAATGGATTTAAACTGGTGTCTTAAATAGGTTTGA70.21257212268448225No Hit
CTCATTTATTATCATTTTTTTCTGTACAAATCTATTATTTCTAGATTTTTGTATAACATGATAGACATAAA70.21257212268448225No Hit
AGGAATCCAAGAAAATGGCCAGTAAGATCAATGTGACGGCAGGGAAATGTATGTGTGTCTATTTTGTAACT70.21257212268448225No Hit
GCTTTGAAGGGCAAATGACAGAGGATAACATAGAAGTTGGAATCTGCAATGAAGCTGGATTTAGGAGGCTT70.21257212268448225No Hit
GGTGTTTTCACAGTCCTGTGTTTGGTATGGAGAGTGTGGAATTGCATATGGGGACAAGAGGTACAATTGCG60.18220467658669906No Hit
TCACAGATGTGGAATATTTGTCATAAATAAATAATGAAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.18220467658669906No Hit
GTGTGAGCCTGGGCATTCCCAGAGAGGAAGGGCCGCTGTGCACTGCCCGGCCTTCAGAAAGACAGAATTTC60.18220467658669906No Hit
GGAAGTGGTTCAGTCAGGTGGCAAAAACATTGAACTTGCTGTCATGAGGCGAGATCAATCCCTCAAGATTT60.18220467658669906No Hit
GAGTTTGCCTTCCTTTCTCCAGGACCAATAAAATTTCTAAGAGAGCAAAAAAAAAAAAAAAAAAAAAAAAA60.18220467658669906No Hit
GTAGTCGCTGTTGGATCGGGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGGAGA60.18220467658669906No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.18220467658669906No Hit
GTTTCTATTTATTTCACTATTATCCTTCATAATTTATATATATGTTACATATAGTTTATATGCGTTAACTA60.18220467658669906No Hit
GGACCCTGTGGCTGGGCTGGGAATGGAGCTGTCAGCACTCTGCGTGGGAGGCCTGGGCCTGTGCCTGCATC60.18220467658669906No Hit
GGACCACGGGCGAGGACGTGAAGCCTCCCGAACACGCACAGAAGGAAGGAGCCAGCTCCCAGCCCACTCAT60.18220467658669906No Hit
CATATAAATATTAGTATTCTGACATGTAAGAAAATGTTACGGCATCACACTTATATTTTATGAACATTGTA60.18220467658669906No Hit
GTCCCAGCTGCGCGTCGCCAAAGTGACAGGCGGTGCGGCCTCCAAGCTCTCTAAGATCCGAGTCGTCCGGA60.18220467658669906No Hit
GGCATATCGGCCGCCCAAGTGACCCTGTCCAGTGTCTGCTTGCCATCCTGCCAGAACAGGCCCTCAAGCCC60.18220467658669906No Hit
AGTTACATTGTCTTACTCTCTTTTACTTCTCAGACACTTCCCCCACCCTCATAGAACCTGTTGCATGCAAC60.18220467658669906No Hit
GATACCATCAAGCCTGATGTCCAAAAGAGCAAAGAATATTTCTCCAAGCAGAAGTGAGCGCTGGGCTGTTT60.18220467658669906No Hit
GTTTCTCTACAGTTGTGCATAAATGTTTTTACTATAAAATGAGCTAATGTATAAAATACTGCTGTATACCA60.18220467658669906No Hit
GCCAGTCAGATGTTTCTCATCCTTCTTGCTCTGCCTTTGAGTTGTTCCGTGATCACTTCTGAATAAGCAGT60.18220467658669906No Hit
CTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTTT60.18220467658669906No Hit
ACTCTTAAATTATTTCCTGCCTTTTCTTCACTCCTATTTACATGTGAAAGCATGTGATCAATTGTTGCTGC50.1518372304889159No Hit
TTCCCTACACGACGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1518372304889159No Hit
GCGTAAGATGAATTGGTATTTGCTTCATAGGCAGTTTGACTGCATGTATTAGAGAATGAAAAGAAGATATT50.1518372304889159No Hit
GGGCTGCTAACTACACAGATTGGGAGAAAATCCCTTCCATGTCGAAGAACCGAGTCCCCGACTCCTGCTGC50.1518372304889159No Hit
TTTGTATTCATTGTATTAAAAGAATCTGTTTAAACAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1518372304889159No Hit
GTTTAGTAGTAGGTGTTAATCAATATGAAATTCTCTGTTTTAAAATAAAAATGTAAAAATCTAAAAAAAAA50.1518372304889159No Hit
CCTGCAGGAGGTGGAGGCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAA50.1518372304889159No Hit
GTTCTCTAGCATAATTTGTATTGAAATGAAACCACCAGTGTTATCAACTTGAATGTAAATGTACATGTGCA50.1518372304889159No Hit
CTTGGAAATGTTATTTCTGCTTTGGCTGAGGGTAGTACATATGTTCCATATCGAGATAGTAAAATGACAAG50.1518372304889159No Hit
GTGTCCAGACCCAGCGGGAGCCGAGGATTCAGGAGAATCTCCCCAGTCAGCTCCAGAGGTGCCCACAGTGT50.1518372304889159No Hit
AGACCCACCTGAGGCTCAGCCCTAGCCCTCAGCCCACCTGGGGAGTTTACTACCTGGGGACCCCCCTTGCC50.1518372304889159No Hit
GAGAGAGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTGGCAGCTTTGAAAAAAC50.1518372304889159No Hit
GCCTCTTGATGCTCATATCTGTTCTTCAGCAGCCTGTCATAGGAACTGGATCCTACCTATGTTAATTACCT40.12146978439113272No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC40.12146978439113272No Hit
ACTACAGCCTGAATTACAATACCAAACAAAAATAACATTGCTACAAGCAATGCCTGTCAAACACACTGCAC40.12146978439113272No Hit
GTTTTCTTCCCTTATCTTACTGTCATTGTCCTAAAACCTTATTTTAGAAAATTGATCAAGTAACATGTTGC40.12146978439113272No Hit
CTTCACAAGATGTTCAAATCGATTTATCACTGAAAGAAGATCTATGAAAATGTGTTTTGACCAAACTTAGT40.12146978439113272No Hit
GATTTTTATTCCTTCCCTATAGAATCTAAAAGAAGACCAGGTATAGTGGCTCACAGCTGTAATTCCAGTGC40.12146978439113272No Hit
GCTGAAGAAAACGGAGACGCAGGAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGA40.12146978439113272No Hit
CTGCTACACCTCAGCCCGGGGCTGCACTGCCACCCTGGGCAACTTCGCCAAGGCCACCTTTGATGCCATTT40.12146978439113272No Hit
TTCCCTACACGACGCGGGTAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12146978439113272No Hit
GGTTGAGGGATAAGGGAGAAGCCAGCAAGCACTCCCCTCCTGGAACTTTACACATGGCTCTGAACTCACCC40.12146978439113272No Hit
CTCCTATTTGTACCTCAACAAATGCAAATTTTCCCCGTTTGCCCTACGCCCCTTTTGGTACACCTAGAGGT40.12146978439113272No Hit
GATTTTGGGGAAGGGTTGAAAGTGGGGGTTTGTTAAATAAGCAATAATTGTTTAAGGAGAGGGGAGTTTTA40.12146978439113272No Hit
CCCTTGGTACAGAGCCGCAGTGTGAGCCTGCGCAGGACAGTTTCAGGTGGTTTTAAAGAACACGTGGAAAT40.12146978439113272No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12146978439113272No Hit
GGGCATCCTGGGCTACACTGAGCACCAGGTGGTCTCCTCTGACTTCAACAGCGACACCCACTCCTCCACCT40.12146978439113272No Hit
ACTTGAAGGAGTTGTTTAAAAGGTCTACCTTAACACAATGTAAAATCTACTATATTACGGAGATGAGTTTT40.12146978439113272No Hit
GGCCCTGCCCCCATGGGCTTTACCCTTCCCTGCGGGCTCTCTCCCCGACACATTTGTTAAAATCAAACCTA40.12146978439113272No Hit
AAATCTAACTTTCTTCCCACAACACTTTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCG40.12146978439113272No Hit
GCAGTGGGACTGCTCCTTTGATATCTGAACCTCTGTTATGGGCTTCTCTGAGACAAGTAAATGTCAGGTGC40.12146978439113272No Hit
GGTTGTAAAGAGTAAATTGTATCTTAGGAAACCAGTGTCACCTTTTTTTCACCTTTTAATTTTATATTATT40.12146978439113272No Hit
AATAGGAAGTTTTGTGAAATGCTGGCCTTGTGTATATCTTAGAATGCAAATTTAATAAAGTGTGTATACAT40.12146978439113272No Hit

[FAIL]Adapter Content

Adapter graph