FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002882811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002882811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1423.308480894687791No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1383.2152842497670084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG451.048462255358807No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA370.8620689655172413No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA290.6756756756756757No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT210.48928238583411No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGC200.4659832246039142No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC170.39608574091332716No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC160.3727865796831314No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG110.25629077353215285No Hit
GGAAGAGTCGGAGGAGTCGGACGAGGATATGGGATTTGGTCTCTTTGACTAATCACCAAAAAGCAACCAAC90.2096924510717614No Hit
TCTGCAGATGGCTAGGGAGTGTGGAGCTGGTACATTTGTCAATTTTGCATCCTTAGAGAGGGATGGAAAGC90.2096924510717614No Hit
ATGCTGTTCTTCTCTCCTAAGTTGAAAATCAGAAACTTGTGGAAAAGAGCACTTGAATGTTGGTACTCTAT90.2096924510717614No Hit
CTTTCTGGTTATATACTGCTTATATCTGTGGATTCAAGTTACTGAAGTGAATACCAATAAAAAGAAAACCC80.1863932898415657No Hit
GCATAACAGGGTCTCCTTGGCAGCTGTATTCTGGAGTCTGGATGTTGTTCTCTAAAGACCTTTAATAAAAT80.1863932898415657No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAA80.1863932898415657No Hit
GTAGTTGGAAACCTTGCTGGTGTATGTGATGTGCTTCTGTGCTTTTGAATGACTTTATCATCTAGTCTTTG80.1863932898415657No Hit
CTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTT80.1863932898415657No Hit
CAGTAAGGATGTTAATGTAAATTTTGAAAAATCCAAACTTACATTCAGTTGTCTCGGAGGAAGTGATAATT70.16309412861137No Hit
ATTTTACACATATTTTTCTACTGTCCAGTGGAAATCATTTTCTGTTTTGGCTAAACAACAAATACTAGTTT70.16309412861137No Hit
GTTCTATATTTCTACATAGTTGAATTATTTTATCATGCTGTTGCTGGGGAATATGACTAACCCTTTTGAAG70.16309412861137No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACGCTATAG70.16309412861137No Hit
GTCTAGAAGTATTGCGTTTAGCCATTTTGAGTACAGCATTAAATTGAGGAGTGGGGAAGAGGGAAATTCAC70.16309412861137No Hit
GCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCT70.16309412861137No Hit
GACCACTGAACCAGACAGCGAAGCCAAGATCATTGTTCTACTTTGTATTTACTACTGTGTGAATCAGTTGA70.16309412861137No Hit
CCTTGTGGGTGATGATCACTGTGCTGCTTGCGGCTCATGGCAGAGCATTCAGTGCCACGGTTTAGGTGAAG70.16309412861137No Hit
CTATTGAAATGGCATCAACATGATGCTGCCCATTCCACTGAAGTTCTGAAATCTTTCGTCATGTAAATAAT60.13979496738117428No Hit
GGCAGGTGCTCAGATGGAAGTAGATTATACAGAGCAACTGAATTTCAGTGATGATGATGAACAAGGAAGTA60.13979496738117428No Hit
GCTAATCTTGGGAATAAGAGGAGGAAGCGGCTGGCAACTGAAGGCTGGAACACTTGCTACTGGATAATCGT60.13979496738117428No Hit
TTCCCTACACGACGCGGGAATGGAAGCGATTCTCCCATGCTCATGTGTAATGGTTTTAACGTTACTCACTG60.13979496738117428No Hit
GTTTTAAACGACTTTTGTTTGCGGGGCTTTTAAAAGGAAAACCGAATTAGGTCCACTTCAATGTCCACCTG60.13979496738117428No Hit
GAAAAAAAATGATTTATTGGACTTTGTTAAGATTAAAAACTCTTATTCTTCTGAGGATACCATTAAAAAAC60.13979496738117428No Hit
GTGGTGTTGAGCTGATTATTAACAGTTACTGAAATCAAATATTTATTTGTTACATTATTCCATTTGTATTT60.13979496738117428No Hit
CTAACATTTTTGGAAAATTTTCTTAATTTCTTTTGATTAAATTATCTTGCCTCTGTTTTATTAGACAAATA60.13979496738117428No Hit
GCTGGCAAAGGAGAAGTAACTTTTGAGGATGTTAAGCAAGTTTTTGGACAGACCACAATTCATCAACATAT60.13979496738117428No Hit
GAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCGCAGTGC60.13979496738117428No Hit
CCCCCCCAATCGGACTGCCAAATTCTCCGGTTTGCCCCGGGATATTATAGAAAATTATTTGTATGAATAAT60.13979496738117428No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA60.13979496738117428No Hit
TTTTATACTCTATGTTTCTGTTTATTTTTAAATCCTTAACAGCAAAATATTTGCCTTTAATTTCTTTTTTA60.13979496738117428No Hit
CTGTTGCTGTATTGATTAGGAAAAGAGACAGAGTAATTTGCAGTTTGTTTGATTTATACTTTTGTTTATCT60.13979496738117428No Hit
GTCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTTGCAGGTGCCAGGAGGCAGAGGTTGCAGTGAGCC60.13979496738117428No Hit
GTACAATGTAACTATCAAAGTTTTATAATTCATTATGAGTTAACCATTTTAATGTTTCCAATTAAACCTCA60.13979496738117428No Hit
CCTTAAAATGCTTAACACATATTTGTTGAATGAATAAGTGCCCCCCAGTGAATTTCCTTTGCCTGTACAAA50.11649580615097856No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA50.11649580615097856No Hit
GCTACTTGGGAGGCTGAGGCAGGAGACTCGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGC50.11649580615097856No Hit
GTGCATCTGTAACTTCAGGAGGATCACTTGAGCTTTAAAACCTGTGCCTTCTCGAAACAAGAATTTGTGCC50.11649580615097856No Hit
GGGCTATCCAGAAAAAAAGCAAAGCAGGTGATATCTAATTTAGGATTTGAAGGATAAATTTGACATTTGGG50.11649580615097856No Hit
GCAGATATAATGGGGAGCCTGAGCATATTGAGAGAAATGACGCACCTCATAAAAATACACAGGAAAAATTC50.11649580615097856No Hit
GCCGCCTCCGGGAGAGCTCCCGCCTCATGTTTGAATACCAGACATTACTGGAGGAGCCACAGTATGGAGAG50.11649580615097856No Hit
GCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTACAGACAGTCAATGTGGATGAGA50.11649580615097856No Hit
AGAGAAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATATGGTGGATGAGAATGGCAAAATTAGTCGCCTT50.11649580615097856No Hit
CTGCCCATGTACCAGCAGCTTCAGAAAGAACTGGCGGGGGCACACAGAACCAATAAGCCATGTGGGAAGTG50.11649580615097856No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACC50.11649580615097856No Hit
GGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATGGATGTGAAATGCCCAGGATGC50.11649580615097856No Hit
ACAAAGACAGGGAGGACAAGAGGAGCGCCCTGTCCTGTGTTATCTCCAAAACAGCTCGTCTTCTCTCTAGT50.11649580615097856No Hit
GCTCCAGGGCTACCTGCGATCTGTGTTTGCTCTGACGAATGGAATTTATCCTCACAAATTGGTGTTCTAAA50.11649580615097856No Hit
GCTTATCTAATTAACTGTGTTTGGAACTGCTATAAATACATCAACAACCGAAACGTGCCGGAGATTGCTGT50.11649580615097856No Hit
GGCCTTCCGGGCAGATGACACCTTTGAGGCCCTGTGCATCGAGCCGTTTTCCAGCCCGCCAGAGCTGCCCG50.11649580615097856No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC50.11649580615097856No Hit
ACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATTTGAAT50.11649580615097856No Hit
ATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCT50.11649580615097856No Hit
GAGTAATCTTTGTAACAAACAGTGCTGGAACAATTGCATAGTCATATGTAAAAAATGAACTATACCCTTAC50.11649580615097856No Hit
GCTGATTAATGGTTTTGCAACTGTAATAGTAGCTGTATCGTTCTAATGCAGACATTGGATTTGGTGACTGT50.11649580615097856No Hit
ATGCTAAACTGAAGAAAGAAATTGAAGAACTGAAACAAGAGCTAATTCAGGCAGAAATTCAAAATGGAGTG50.11649580615097856No Hit
GCCCTACAAAGGCTAGAACACTACAGGGGATGAATTCTTCAAATAGGAGCCGATGGATCTGTGGTCCTTTG50.11649580615097856No Hit
CCCCACGACCTTGGCCCGAGCCCCTCCGTGAGGAACACAATCTCAATCGTTGCTGAATCCTTTCATATCCT50.11649580615097856No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC50.11649580615097856No Hit
GGTTACTGGAGGGCCTTTCAGCCCTCTCCTTGGCACAAGAAGTATGTCAGTCATAAATTATCGTCTTTGTA50.11649580615097856No Hit
GGGTCAGTCCTTCCCGTTCTGTGCTAGATTGCTGTATAAGGACCTCACAGCAACTCTTCCCACTGACGTCA50.11649580615097856No Hit
GCCTTGATACATCATCAAAGGCATAGCCTTTCCAAGATGGTTTATCAGACATCACTTTGTTCTTGTTCTGT50.11649580615097856No Hit
GTGTAATGTACACATATGGGAATAAAGGAAGGGGAAGAAAGAGAGAAAGGAACAGAAGAAATATTTAATGA50.11649580615097856No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGG50.11649580615097856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)