FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002883076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002883076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418
Sequences flagged as poor quality0
Sequence length71
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39816.459884201819687No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG652.6881720430107525No Hit
CCTAATTAAGGTTGATGACAACAAGAAACTAGGAGAATGGGTAGGCCTTTGTAAAATTGACAGAGAGGGGA261.0752688172043012No Hit
ATTTCAGTCTACGTACTTTCCAAGAATGTCACCATCTCTAAATAGGAAATAATCCTTGTCATCTAGAACTA251.0339123242349049No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT230.9511993382961125No Hit
TTCCCACACTACTGAATGGAAGCAGGCTGTCTTGTAAAAGCCCAGATCACTGTGGGCTGGAGAGGAGAAGG230.9511993382961125No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA230.9511993382961125No Hit
CTTGATTCCTGATCATCAGAAATTTCACCAGAAACAACTTGCTTCCAATATACCCAATTCTATATGAAGAA230.9511993382961125No Hit
GTCCTCATCCCCAGGCTTGAAGGCACATGGCTTTCTCATGTAGGGCTCTCTGTGGTATTTGTTATTATTTT210.8684863523573202No Hit
CCCCTGAAAAACTCTGAAGAATCCCCTCCAACCATTCTCTTGTGAACAGAAGAAATCAAGTCCCTCTTCCC210.8684863523573202No Hit
TGTCATCGTGAAGGCCAAATTCTTCAGCAGAAGAGCTGAGGAGAAGATTAAGAGTGTTGGGGGGGCCTGTG180.7444168734491315No Hit
GTGTTTGCTCTGACGAATGGAATTTATCCTCACAAATTGGTGTTCTAAATGTCTTAAGAACCTAATTAAAT180.7444168734491315No Hit
GGCCAGCAGGCCTCTCTGAAGGCCTCCATGGAGCAAACGGAGCCACCTCGGGAAAGAGTTTAATGGAATAT170.7030603804797354No Hit
GAGACGGCGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGATCTCGTGATCCACCCAACTCAGCAT170.7030603804797354No Hit
CCTCCCTCATCTCTATGCTGGAGACTACCAGTGTGAAGTTTCTTTTCAAAACACAAATCAGCTAGGCACAG160.6617038875103392No Hit
GCCTATAAAGCCCCCTGTTTTATGCCCTTCTAATTCTGATGTTTGGGTATTGTGTGAGTGCATGTGTTTTT150.620347394540943No Hit
CGACGCGGGGGGCTGTGGTAAACGGGAGAGCTTGTGTTTTTGAAGTGGAAAAAAACCCAAGAGTTTGTACA140.5789909015715468No Hit
GGCTGGTCTCAAACCCCTAGGCTCAAGCCATCCTCCCACCTCAGCCTCCCAAAGTGATGGGATTACAAGTG140.5789909015715468No Hit
GGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTG140.5789909015715468No Hit
GGATACATCTATTTTTATATATTAAATTTGAATGTGTTATCAAAATGCTTGGTTAACTTAAGGCACCTTTT130.5376344086021506No Hit
ACCAAGCTGTTGACTTTCTTACTACTTGCAGTAGCCTGTCCCCAACTTTTCCATCCAGTGCTTAACCTAAA130.5376344086021506No Hit
GTTTACTGAATTTTTATATCTGAGTGTTCAAAATATTTCCAAGCCTGAGTATTGTCTATTGGTATAGATTT130.5376344086021506No Hit
GCTTCCAAAGGCACAGGCCAAGTTGTAGCTTTGTCCCTTGCCATCATGCCCAACAAGAGGTTCTATACCTT120.49627791563275436No Hit
ACAGGGGGTGGAGAGACCAGCCTTTCTTCCTTTGGTAGGAATGGCCTGAGTTGGCGTTGTGGGCAGGCTAC120.49627791563275436No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA110.45492142266335817No Hit
AATAATAAACTATTTACACTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.45492142266335817No Hit
TCCAAAAGTAGCCCAGGGCTGTAGCACAGGCTTCACAGTGATTTTGTGTTCAGCCGTGAGTCACACTACAT110.45492142266335817No Hit
TTTCCTTCCTCATTGCTTTTACCCAGTCTGCTGTTTTGGGAAGCCCTGACATGTATTTTGGTGCCTACATA110.45492142266335817No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA100.41356492969396197No Hit
GGTGGTGAACATCCCGTCCTTCATTGTCCGCCTGGATTCCCAGAAGCACATCGACTTCTCTCTGCGCTCTC100.41356492969396197No Hit
GCTTTGAACCTCAGTTTCTTTATCTGTAAAACGGGATAACTATCAACTTCACCAGGTCAAATGCATATGCT100.41356492969396197No Hit
GAATTGGGATATATTTGATATACTTCTGCCTAACAACATGGAAAAGGGTTTTCTTTTCCCTGCAAGCTACA100.41356492969396197No Hit
GCCCTCTGTGTCAGGCCCCTCATTAACAAAATGGAGTTTATGATGGCATCTACCTCACATGGTTATTGTGT100.41356492969396197No Hit
GGTATATAAGTGTGATCCTGCAGGTTACTACTGTGGGTTTAAAGCCACTGCAGCGGGAGTTAAACAAACTG100.41356492969396197No Hit
CCTTAATGATTGGTTGCTTAGCTCTAGCAGTGTCTACAGAAATTAAAGTTGACAAGAATGAAATAGAGGAT100.41356492969396197No Hit
GATGTGGAATATTTGTCATAAATAAATAATGAAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.41356492969396197No Hit
GTGGATGTGATGAAGCCAATAATTCAGGACTTATTCCTTCTTGTGTTGTGTTTTTTTTTGGCCCTTGCACC100.41356492969396197No Hit
GCCCAACACCTGGCTTGGGCTCACTGTCCTGAGTTGCAGTAAAGCTATAACCTTGAATCACAAAAAAAAAA100.41356492969396197No Hit
CACTATGAGATCTGAGGAGGCTTCGTGGGCTTTTGGGTCCTCTAACTAGGACTCCCTCATTCCTAGAAATT100.41356492969396197No Hit
GTTAACCAAGCAGAATACACAGATATTTTGCTTTACAACTTGCACCTAAAATACCAGTATACGTAGCTGGT100.41356492969396197No Hit
GCATTGCCTGGCCTTCCTGTCTAGTCTCTCCTGTAAGCCAAAGAAATGAACATTCCAAGGAGTTGGAAGTG100.41356492969396197No Hit
GATGTAATCATAGCATGCACTTGTTTAAAGGATATAGGAATCTGAAGGAGTGCAGCTTCTTCATGGTTTCT90.37220843672456577No Hit
TTACTGTTGATGACTTGGTGGCTGAAATCACTCCAAAAGGCAGAGCCCTGGTACCTGACAGTGTAAAGAAG90.37220843672456577No Hit
GAGCCAGGATCATGCCACTGCACTCCAGCCTGGGCGACGGAGTAAGACTCTGTCTCAAAAACAAGAAAATT90.37220843672456577No Hit
TTTAAATATAAAGTTTATAAAATAAATACTTTTTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.37220843672456577No Hit
CATGTAGTGGTTATTATTTAGAGTTTATAGCCGTATTGCTAGCACCTTGTAGTATGTCATCATTCTGCTCA90.37220843672456577No Hit
AACTATTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.37220843672456577No Hit
GCATTACCACTTAAAGAGGAACCAGAGCTTCTGCCCAACTGTCAACCTTGACAAATTGTGGACTTTGGTCA80.3308519437551696No Hit
GAAAAAACATTTCCTTTAAAAATTCCCCTTAAATGTAGGTTTATAAAATGTCAGAAATGCCTTGTACCCAA80.3308519437551696No Hit
ATTGAACTGTCTTAAGGATTTGTTTAAACAGCTAAGTTACTTGATTAAAATAAAGATAAAATTGTAAAAAA80.3308519437551696No Hit
CAGATTTTTATGTTTTCTTTCTCACCCAGGCAATCTTGCCTTCTGGTTTCTTCCAGCCACATTGAGACTTT80.3308519437551696No Hit
GGAATAAAGCTGTATTCCAGTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.3308519437551696No Hit
CCTCTTGATCCAACCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAACCACCGCGCCCGGCCTAA80.3308519437551696No Hit
GTGTAACCATTAGTGCAACTACTACCTTGGTGCCTCAGTTTACCCATCTGTAAAATGGGTAAGCATAGCCA70.2894954507857734No Hit
GCATCCTTTGCATTACTTTTTCCTTCAGGATGATCTAGAGCAGCATGGAGCTGTTGGTAGAATATTAGTTT70.2894954507857734No Hit
GCTGGCCCACCTGGATCCTGGCCAACTCGGGATTGAGTTCGTTCCTGGTCTCAGAAGGCCCGTTTTGCTTT70.2894954507857734No Hit
GCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACT70.2894954507857734No Hit
GAGCTCCTATTTTGTACTCCTGGCTAGAATGCTGTGGAACAAATACAAAGTGAAAAAAGTTCTCTGTAGAT70.2894954507857734No Hit
GCAAATGGACGCTAAAAGCAAGCAGGATTAGCTGTTCTTATGTCAGACGAAACAAACTTTAAAGCAACAGC70.2894954507857734No Hit
GAAGCAATAAATCGTCTTATTTTATTTTCTTTTCCTCTCTTTCCTTTCCTTTTTTTAAAAAATTTTACCCT70.2894954507857734No Hit
GGACTTGCACTTGTTCCTTGTTTCCTCTGGGTTTTATAGTGATACAGTGGTGTCCCCGGGGCCAGGCGCGC70.2894954507857734No Hit
GTTTTCTCTTAAAGCCATTTCCATGATGATGATGATGGGACTCAATTGTATTTTTTATTTCAGTCACTCCT70.2894954507857734No Hit
TGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT70.2894954507857734No Hit
AATAAATTGTCACATTCCGAAGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.24813895781637718No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.24813895781637718No Hit
GAATCACACTCTCACATACTGTCTTGTACTGACACATCCAAAGCATGAGTGTGTCAGAAATCCCTTGTCTA60.24813895781637718No Hit
AGAAAGGCCAACTTCAAAGACCAGTGAGAACCAGCTGCCCCTGCTTCACACCTCTGCTTGGAGAGGACAAG60.24813895781637718No Hit
GATAAGTAGTTTTAATGGGGAGACCTTATTTTTGGAAATGGAATTATTTGTAATTATAACAAGGAAGAAAA60.24813895781637718No Hit
GCCGGACTGCGAGTCTCTTTGCGGCGCTACACTAGAGCAGAGTACGAGTCTGAGGCGGAGGGAGTAATGGC60.24813895781637718No Hit
GGCTGGCTATAAGAAAAAATTATGGAAAAAGACACCTGCAAGGAAGAAGCGATTGAGGGAATTTGTATTCT60.24813895781637718No Hit
ACTGAATGTTTACAAATTGCTTGCCTGCTAAAGTAAATGATTAAATTGACATTTTCTTACTATAAAAAAAA60.24813895781637718No Hit
GTACAATGTAACTATCAAAGTTTTATAATTCATTATGAGTTAACCATTTTAATGTTTCCAATTAAACCTCA60.24813895781637718No Hit
GCATATATTGCATTTAAAGAAAGATAAAGCTTCTGAAATACTACTGCAATTGCTTCCCTTCTTAAACAGTA50.20678246484698098No Hit
TCTCTATGTTGCCCAGGCTGGTCTCAGGCTCCTGGGCTCAGATGGTCCTCCTGCCTCAGCTTCCAAAGGCA50.20678246484698098No Hit
CCCTACACGACGCGGGCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20678246484698098No Hit
GATTCCCCCTATCTTGGCATTTGGCGGCAGACGACCGAGCTATCACAGCCTCAGATAAATACCAAAGTGGA50.20678246484698098No Hit
ACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAAGCAGAGGTTGCAGTGATCCGAGATCACACT50.20678246484698098No Hit
AAATAAAACCTCAGCTAGCTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20678246484698098No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20678246484698098No Hit
AAACACACCTCGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20678246484698098No Hit
GTTTAAAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20678246484698098No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT50.20678246484698098No Hit
GGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTTTTATCC50.20678246484698098No Hit
CATATTGAGCATATTGTTAAGTGGGTAAAGCCAAGTAAAGGTACTGGGTGTTTTTGTGAAAAAAAAAAAAA50.20678246484698098No Hit
GTCTTTGATTAAATAAAAAGGAAAACCAGAAAAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.20678246484698098No Hit
CAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTAT50.20678246484698098No Hit
GATTTGGGCCCCTCATCTTCATTTATTATATTATCAAAACTGAGAGGGATAGGAAAGAAAAACTTATCCAG50.20678246484698098No Hit
GTTTCTTGTCGTGAGGCACCTGCCATGTCTGTTGCGTTTTCCTCGGCAGCGTGTAGGGCTTCAGCTTCCTG50.20678246484698098No Hit
GAACATTTACTTAACACATCATGAAGGCTAGTTTGGATGTGGAGGGGAATAGACTCCATCTATCCATATGA50.20678246484698098No Hit
AAGCTGTACTAGTTCATTTCATAAGGAAATGATACTGTAGACAAATGTAAATAAAGCCTGTGAGTCAAGCA50.20678246484698098No Hit
GCTCCGTCACGGCCGCTCCCGAGAGCCCGAGGTCCAAGCCCAACACGACTTGGAATAAATGATCAAGTTAT50.20678246484698098No Hit
GGTGACACCTCACACTCAGTGCCGGTTAAAATTACTGAAGTTAGAGAGAATTAAAGACTATCTTCTCATGG40.1654259718775848No Hit
GTCCATGGGGTCTGCGGGGTCTGCGGGGTCTGCCTGGCCTGTGGGTTCTGCCGGTGGGGCTTCAGGAGTAA40.1654259718775848No Hit
CACCGCAGAGGTACTTGAACTGGCAGGAAATGCATCAAAAGACTTAAAGGTAAAGCGTATTACCCCTCGTC40.1654259718775848No Hit
GGTCCCCTAGTTGAGGATAGTCAGGAGCGTCAATGTGCTTGTACATAGAGTGCTGTAGCTGTGTGTTCCAA40.1654259718775848No Hit
GTCATATGTTCATGGTCAGATGTCATACATTCTCAGCTGTCTTGTTCCACCAGTATTTACCAGGAAAACAA40.1654259718775848No Hit
TGTCTGCTCCACCCCCACTTCCGGAGGCAAGTGTCCATCTGCCTTATAACCTCCGCCGACCCCATGCCTGT40.1654259718775848No Hit
TTTTATTAAAATGTAGAATACAGAAAGCCTGATCTGGAACGCTCTTTCCGTGGATAGTATACAATAAACAG40.1654259718775848No Hit
ATGTTAACCAACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1654259718775848No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC40.1654259718775848No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1654259718775848No Hit
CATTGGGAGCCTCATCTGCAATGTAGGGGCCGGTGGACCTGCTCCAGCAGCTGGTGCTGCACCAGCAGGAG40.1654259718775848No Hit
GATAAGGATAGAGGGGAGGCCTTATTGTATCGGTTTTATATTACCTGTTCTGCTTCACCAGGAGATCATGC30.12406947890818859No Hit
GTAAATAGAGCCATTGGATCTTTATTTTTGATTAATTTGTTCTGATTTTTTGGTTTGTTTTTTAAGGAACT30.12406947890818859No Hit
CCTCTGCCCAGCTTGGGCCCAGCTGGGCTCTGCCACCCTACCTGTTCAGTGTCCCGGGCCCGTTGAGGATG30.12406947890818859No Hit
AAGCAAAGAAAATAAAAATAACACAGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12406947890818859No Hit
TCTCTATGTTGCCCAGGCTGGTCTCAGGCTCCTGGGCTCAGATGGTCCTCCTGCCTCAGCTTCCAAAGGCC30.12406947890818859No Hit
CTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC30.12406947890818859No Hit
GTGTGTAAGAATGCTAAATAAAATGTTATACAAGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12406947890818859No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCAG30.12406947890818859No Hit
GGGCGCAATGGCTCACGCCTGTAATCCTAGCATTTTGGGTGACTGAGGCGGGTGGATTGCTTGAGCCCAGA30.12406947890818859No Hit
AGGTAATAAATAAGGGTTGTCCTTCACTGATTAAGGGAATAAACTTCAATAAACTTAACAACAACAAAAAA30.12406947890818859No Hit
TACATATATACATAATGTGCTTAACCACTGCCTTTTAAAACTTTAAATTAAATAAAACATTGGGGTTGATT30.12406947890818859No Hit
CTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGCATTTTGTAGATGTGGTTTGAC30.12406947890818859No Hit
GAGCATTAATCATACACCTTATTAACGGTGAGGTGAATAACTTTGAAATAAAGTTTTAGAGAAATGTTTCA30.12406947890818859No Hit
AATTAAATGTTTTACATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12406947890818859No Hit
GAGTTCTCCATCACCTACAAGCCCGTAAAGCATGGCCGGCCCGGCATCGGGGCCACCCACTCCTCCCGCTT30.12406947890818859No Hit
GAAATAAACAGCTTTATTGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12406947890818859No Hit
AAAGTATTAAAAGTAGCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12406947890818859No Hit
TTTCTAGGGGTATTCATTTGCTTTCTCGTTGAAACCTGTTGTTAATAAAGTTTTTCACTCTGGAAAAAAAA30.12406947890818859No Hit
ACTGTAATCACAACATCCTGACTCCGTCATTTAATAAAGAAGGAACATCAGGCATGCTAAAAAAAAAAAAA30.12406947890818859No Hit
GCTCCCCGAATTCTCGAGGAGACTGATTTGAGTGATAATAAAACTCCAGTCTCCCGCAAAAAAAAAAAAAA30.12406947890818859No Hit
TAGTGATTTGGTGAGAAATCGTTGCTGCACCCTTCCCCCAGCACCATTTATGAGTCTCAAGTTTTATTATT30.12406947890818859No Hit
GTCTTGGAATAAAGTCTATTTTCTGCCTTTTGGTTTTTAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAA30.12406947890818859No Hit
GGATGTGGCCCAGAGGTGCAAGGAGCTGGGTATCACCGCCCTACACATCAAACTCCGGGCCACAGGAGGAA30.12406947890818859No Hit
ATGTTACTAAATGGTGTTACATTAAAGCCCTGTGTTAAGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12406947890818859No Hit
GGCATACTTTGGAGATGTTATGGGTTCGGTTCCGCATCACTGCAATAAAGTGATGCTACTAGGAGGTGGAG30.12406947890818859No Hit

[OK]Adapter Content

Adapter graph