FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002883249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002883249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11788
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6495.505598914149983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310.26297930098405153No Hit
GTGAAGTTCTTTTAGGACAGATAAATATGCCTCAGGGCCAGCTTATCTCTTTGAACATCGTAACTGGATAT220.1866304716661011No Hit
AGGCTGACCTGCCGGGACCGGAGTGCCCGGGGACGCTGTGCCCCCACTTGCCCAACGTGCGGAATCGGCTA220.1866304716661011No Hit
GCACAGACTTTCTATTATATTTTGCATTTGTAAAACTAAGTGAACTGAGTCTATTCCTGTATAACAGAAAC200.16966406515100102No Hit
ACCCACTAATGAGTCATAAACTTATATTTTGAAAAACATAGGTTTAGTTAGTAAGAGCTTGGGCTCTGGGT200.16966406515100102No Hit
CCTCAGGGCCTCACCTCTCTGCCCTTCCCTGCCCGGTTCCTACTCACCTGGCTAGGGCCAGTGCCCATTTT200.16966406515100102No Hit
AGAGAAGATAGGGAAATTAGAAGATAAAAACATACTTTTAGAAGAAAAAAGATAAATTTAAACCTGAAAAG200.16966406515100102No Hit
CTTTTTCGTGTGTTTGAAATGGTCGCATTGGTGTCATATTTACATGAGAAAGTATCTTACGCAATTTTCCA190.16118086189345096No Hit
CATATAGGTATGTTTGCATAGCTATAGAAAAATACTGGAAGAATACCCATCAAACTATTAACAGCAATTAT180.1526976586359009No Hit
ATATATTGATGATTAAGATGTATTTTATTTATTTTTATCCATAGCTTTTTCCATATAAGTATGTATCTTAG180.1526976586359009No Hit
GTTAAAAACAATGCAAATTCACTGTAGTGAAGACAAGTTGAACCAGATACGGCAGAGGATGTTTGAGGACT170.14421445537835087No Hit
GTTACAGGCTCCTGCCAGACATCACCCTGCTTGAGCCCGTGGAAGGGGAGGCAGCTGAGGAGTTGAGCAGG170.14421445537835087No Hit
GTGATTTGGTGATAAGTCTCTAAATTTGTTCAGCATGTTAATCTCTGCATAGAGAGCCTTCTAGTTACAAT160.1357312521208008No Hit
CTTTTAAAACTGACTTGGGTGGGGAAAGTGAAGTATGCTTCTATTTAATTCTATGTGTACCTTAGCTAACT160.1357312521208008No Hit
GGTGGAGGTGGGAGGATTATAAATAGAGACTTTCCTTAAGACTTTAAAAATGTATTTAAAACTATTTTTTA160.1357312521208008No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT160.1357312521208008No Hit
CTCTATTAATTGGGTAGGATTTCACCCATTCCTAGCTAAGTTCTTAAAATTAAACCCTTTGGTTCGTGTTT160.1357312521208008No Hit
GCAAAAATCTGAATATTTATATTTCTTGTTTTTTTCTTTATATGTTTTGCATTTTAATATGTTGAGCCACT160.1357312521208008No Hit
GGAAGGGAGATTTCCCCAAGGGAGAAGACACCAGAGGTGATTGATGCCACTGAGGAAATAGACAAAGATCT160.1357312521208008No Hit
CGCTAAGGCATGCCAGGTTTTCTGAGGCTCCAGCCAGCTATATAATATGGCCCATTCTTATGCATATTTTT160.1357312521208008No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG160.1357312521208008No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTA160.1357312521208008No Hit
GTGTTTACATCTTCCGTGTCAAGATATTTTAAAGCATGAAAATAAAGCCATTCTTGCCCTTTTTCGTGTGT150.12724804886325075No Hit
GTTCTAAGATCCCGAAACTCTGTTTTGTAGCATTCCTTCAGCAAAGCAACCTAAAACCTGTAGTTAAAATT150.12724804886325075No Hit
CTTTTTCTACTCAACTTTTAGGGAGAATGTTAGAAAGACTTGAAGTTTAGAAAGGAAAATGGTTTAGTAAT150.12724804886325075No Hit
GTGTAGTATCATTATAAGGATAGGCATTAACATCAATAGGACAGGAATGAGTGTCCAGAAATAAATCCTTT150.12724804886325075No Hit
AGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTTCTTTTTTTTTCCTTTTTTGTCT150.12724804886325075No Hit
ATAACTTCATTGTTTGGACCTGCATATTTAGCTGTTTTGGAACGCAGTTGATTCCTTGAGTTTCATATATA150.12724804886325075No Hit
ATATTTAAGTGTTACTGTGGCTTCAAAGAAGCTATTGATTCTGAAGTAGTGGGTTTTGATTGAGTTGACTG150.12724804886325075No Hit
GTTCAATGTATATTGACTTCCATACTGGTTTTTCCAAAAACCAAAGGTAGCTTTGAAAAACCATGTCTGGA150.12724804886325075No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGC140.11876484560570072No Hit
CCCGGAGGCTGGGCTGGGCGAGTATCTCTTCGAAAGGCTCACTCTCAAGCACGACTAAGAGCCTTCTGAGC140.11876484560570072No Hit
CTGTTATGTTTTCCAGTAAACTAGAAGTACGATATTTGATAATTATATTTGTATTTCACCACCTAAATGTA140.11876484560570072No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCC140.11876484560570072No Hit
GCCTTACAACCATAGCTTAGGGAATTTTTTGCTAAATAGAGCTGGCTTTCAAAAGCCATGTCGCAATTCAG140.11876484560570072No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGAGGCG140.11876484560570072No Hit
ATATTAGGTATGTTGCACTTCCCAACTTTGGTGTTACACATTTGCTTTAGAATTAACATGAAAGTTAGACC140.11876484560570072No Hit
GTGACTCTGAGAGTGAGGGGCCTCAGAAGGGGCCTGCCAGTGACTCAGAAACTGAGGATGCGTCCAGACAC140.11876484560570072No Hit
AAGTGGGAAATGTTGCTGGAACTGAAATCATGGGATTGGGGAAAGCAAACCAAAAAATGCCCTCATAATAT140.11876484560570072No Hit
GTGGAAGGCCTTCCGTTTAAAACCTTGCTCTTTCAGGCTCAGCACGCATCTCATCCCCAGGATGGATTTGC140.11876484560570072No Hit
GTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATAT140.11876484560570072No Hit
CTTGTGGTGTAAATTTTTTTTTATTACACCAAATTATGTTCACACAAAACCTTTGTAATCAGGGGGAATTA130.11028164234815066No Hit
GCTTAAGTGTCTTCAGTTCAAGATTGATAGAGCCCTTGGCATTTTATTATCACATTCTTAGTTCTCAGGTT130.11028164234815066No Hit
AATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATA130.11028164234815066No Hit
TTCCCTACACGACGCGGGGGAGCCATTGTGGTTCAGGCCGGTTGCGCCTGCCCTCGGGCCCTCACGGAGGC130.11028164234815066No Hit
TCCCTACACGACGCGGGGGAGATGGCCTTCTGGTAACTGAATATTACCTTCTGTAGGAAAAGGTGGAAAAT130.11028164234815066No Hit
CTACTAAAAATATAAAAAATTAGCTGGGCGTGGTGGCGGATGCCTATAATCCCAGCTACTAGGGAGGCTGA130.11028164234815066No Hit
GGGTTGTATACAAACTATTGCAAACACTTGTGCAAATCTGTCTTGATATAAAGGAAAAGCAAAATCTGTAT130.11028164234815066No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATTGCGATTAGAATGGGTACAATG130.11028164234815066No Hit
AATCTAGTGTGCAAGAAGAATGTGTTTCAACAATCTCAAGCAGTAAGGATGAAGATCCTCTAGCTGCCACC130.11028164234815066No Hit
CATATTTATGCTGTCTGAAGGTCACGATCATGTTACCATATCAAGCTGAAAATGTCACCACTATCTGGAGA130.11028164234815066No Hit
GTTTAATAGGTTTCAACTGTCAATGTGCATTAAAATTTCCTGGGAGCAGGGGGATGGGCTTTTAAATTCTG130.11028164234815066No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACC130.11028164234815066No Hit
CCAGGAGCCTGTGCTATTGCTGATTACATTTTATAGTTAGTCAGGGGACATTGTCAGCTCAGTAACCCTTT130.11028164234815066No Hit
ATTGAAAGGTCAAGAGGTCAGCGTTTTTGCCTTATTTCCAAATTTGGTGATTTTTTTCTTAATAAAATATA120.10179843909060061No Hit
GTAATAAGCATGATGGTGCCTTCTATTAATACATCATTCCAGTCTTGCTGGTAATTTTGTACAGTATAGTG120.10179843909060061No Hit
GTGACATATAATTGCCATACCATTCACCTATTTAAAGTGTGCAGTCTAGTGGTTTTTGATAGTGAAGAATA120.10179843909060061No Hit
CAATTACGTGATCATGGCAAAACTGTTGGAGTAGTTGATACCCGAAAAAAGACTAAATTAATTTCTCCTCA120.10179843909060061No Hit
CTGTTTGGAGGCCTTTCAAGATCTCTCTTAACTCACTGTGTTACGGAGATATGGACAAAACCATTAAGGTG120.10179843909060061No Hit
CCTGTAATCCCAGCTACTCGGGAGGCTAAGGCAGGAGAATCACTGGAACCTGGAAAGCGGAGGTTGCAATG120.10179843909060061No Hit
GTTCCTTTTAACAGCTTTTCTTCCTCATCTGGTGGAGCATTGCTGCATGCTCATTCCCAGATTTTAAATCT120.10179843909060061No Hit
GGAATGACACCTCTGCAAGCCAAGGAACACGGAGCATTGCTGGGAACCACCGGAAGAAGCCAGAAGACAGG120.10179843909060061No Hit
GTTCTGGACCTGCTTCTTACTCAGAAGCTCACAGCTTGGGAAAGCGTCTCTAGCAGCTGATATCCCCAGAC120.10179843909060061No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG120.10179843909060061No Hit
TCAGAGCCCTTGCCCGCTCGGGTATGAAGATCGGGCGGATTGAGGATGTCACCCCCATCCCCTCTGACAGC120.10179843909060061No Hit
GTATTTTATGATACTTGAATTTTTTGAAATGGGCATTTATTTTATAACATGTTAAAATGTACTTTTTAAAT120.10179843909060061No Hit
GGCTATCATTGATCCAGGTGACTCTGACATCATTAGAAGCATGCCAGAACAGACTGGTGAAAAGTAAACCT120.10179843909060061No Hit
GTGAAACCCCATCTCTACTGAAAATACAAAAATTAGCTGGGTGTGGTGGCCGGTGCCTGTGATGTCAGCTA120.10179843909060061No Hit
CCTCAATAGGACCAAGGTGCTGGGGTGTTCCCCTCCCAACCTAGTGTTCAAGCATGGCTTCCTGGCGGCCC120.10179843909060061No Hit
TCTTCAGGCCAACCTGGTGGAAATGTTGTTCTCTGAAGATTAAGATTTTAGGATGGCAATCATGTCTTGAT120.10179843909060061No Hit
GTGTTAGCTTAGTTCTCTTTTGATGTAGGTTATTATGATTAGCTTTGTCACTGTTTCACTACTCAGCATGG120.10179843909060061No Hit
AGACTGAAATAATAAGTGTTCATTCTCCAGTAACACTGGAATTAAACTCGAAACTTACAAAAATGTACATC120.10179843909060061No Hit
AATCTAAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAGAGGGAG120.10179843909060061No Hit
CTACAGGAGGAAAAGCAAGGCTTACAGAAGGATGTTCCTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAG120.10179843909060061No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACG120.10179843909060061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)