FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002883270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002883270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35359
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5491.5526457196187675No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA3741.0577222206510366No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG2330.6589552871970361No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC1720.48643909612828423No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT1660.46947029044939054No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCC1420.40159506773381604No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAA1400.39593879917418473No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT1320.37331372493565995No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT1060.2997822336604542No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC900.2545320851834045No Hit
ACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATT890.2517039509035889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790.22342260810543282No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCC750.2121100709861704No Hit
GTTCTACACCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTATTCACTATAACTTAACACTAC720.20362566814672362No Hit
CTCTAATTAATTGTAATCTGAATAAAGTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAA650.18382872818801438No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCC640.18100059390819878No Hit
ATGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTT600.16968805678893634No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT590.16685992250912074No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCC560.15837551966967392No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAAT490.13857857971096468No Hit
CCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTTTAAGCATATTA490.13857857971096468No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGC470.13292231115133346No Hit
TATCACAGCAGGTTACAACAACTTTGGGATAAAAGGCAACTGGTAAACTGTCCAAAACAAGGTTCCAAATA460.13009417687151786No Hit
GGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTTTTATCCCAAAG460.13009417687151786No Hit
GTCCAATTAGTTTCCTTTTTAATATGCTTAAAATAAGCAGGTGGATCTATTTCATGTTTTTGATCAAAAAC440.12443790831188666No Hit
GAATAAAGTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCC440.12443790831188666No Hit
AATTAGTGTTCTACACCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTATTCACTATAACTTA430.12160977403207106No Hit
ATAGAAAATGGTCCAATTAGTTTCCTTTTTAATATGCTTAAAATAAGCAGGTGGATCTATTTCATGTTTTT430.12160977403207106No Hit
ACATGAAATAGATCCACCTGCTTATTTTAAGCATATTAAAAAGGAAACTAATTGGACCATTTTCTATTTGT410.11595350547243984No Hit
GTGTAACAATTGTGTAGCCTTTTTGTATAAAATAGACAAATAGAAAATGGTCCAATTAGTTTCCTTTTTAA380.10746910263299302No Hit
TGTATGGGTAGGGTAAATCAGTAAGAGGTGTTATTTGGAACCTTGTTTTGGACAGTTTACCAGTTGCCTTT380.10746910263299302No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.10746910263299302No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCC370.1046409683531774No Hit
GTGTAGAACACTAATTAATTCATAATCACTCTAATTAATTGTAATCTGAATAAAGTGTAACAATTGTGTAG370.1046409683531774No Hit
GATTTACCCTACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCACCTGCTTATTT370.1046409683531774No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGA370.1046409683531774No Hit
CCTATAAGTCTAATGGCTTTCAAATGTAATTTCCATTTGCTAATGGTGATCTTGCCACATCTGGCACGGAG360.10181283407336181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)