FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002883339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002883339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG1424.362519201228879No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA962.9493087557603688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310.9523809523809524No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA270.8294930875576038No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA250.7680491551459293No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC180.5529953917050692No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT170.522273425499232No Hit
GTTTTATAAAAAGTCATGTTATCTCATGAGCTGCTTCCCACCTGGCTGTATAATTTTATCATCATGGTTCC120.3686635944700461No Hit
CAGAGCTATTTATTTGTATTTAAAGCACTGTTTATTATTTGTACTGATTCTTATCCCTCTGTGTGAATAAA110.3379416282642089No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAA100.30721966205837176No Hit
GATGTAGATTCTCTGGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAG90.2764976958525346No Hit
GAGTGAAACATTCACAAAGATTTGCGTTAATGAAGACTACACAGAAAACCTTTCTAGGGATTTGTGTGGAT90.2764976958525346No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGG90.2764976958525346No Hit
ACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTAGCAGTAAA80.2457757296466974No Hit
GCCCACCTGGGGAGTTTACTACCTGGGGACCCCCCTTGCCCATGCCTCCAGCTACAAAACAATTCAATTGC80.2457757296466974No Hit
CACAACTTCTTAAGCTAAATGGTATTTTCATTTTTCTCAAGCTCTCCAATAAATATGACCACCAAAAAAAA70.21505376344086022No Hit
GGCTTTCGCTCTAAGATTAAAAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC70.21505376344086022No Hit
GCCTTCCAGTTTGCTTGATATATTTGTTGATATTAAGATTCTTGACTTATATTTTGAATGGGTTCTAGTGA70.21505376344086022No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT70.21505376344086022No Hit
ATGTTTTATTTTTATTTATCTTAATTATACAAGTAGCACACAAATATATTCCCACATAAAAAGTCCAACAT60.18433179723502305No Hit
GATAAAAGAAGTAAGGCTTTGAAAGCTTCAGCGCCTGCTCCTGGTCATCACTAACCAGATTTACTTGGAGT60.18433179723502305No Hit
AACTCGTTCTGCTGTCAATCTGAAAATGCCAGTGCTGTGCCTTGGAAAGAATGTTTGGCTTTAATTTAAGG60.18433179723502305No Hit
GCCCCAGCTAAGGTTGAAGCCAAGGAAGAGTCGGAGGAGTCGGACGAGGATATGGGATTTGGTCTCTTTGA60.18433179723502305No Hit
CCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAG60.18433179723502305No Hit
GTAGTTGGAAACCTTGCTGGTGTATGTGATGTGCTTCTGTGCTTTTGAATGACTTTATCATCTAGTCTTTG60.18433179723502305No Hit
TTATCACTGACCCCACAGAAATATAAAAATCTCTCAGTGACTACTACAAACACCTCTATTGCAAAAAAAAA60.18433179723502305No Hit
GTAAATGCTCAATAAATATTGATGATGGGAGGCAGTGAGTCTTGATGATAAGGGTGAGAAACTGAAATCCC60.18433179723502305No Hit
GGATTTCCTCCAGGGAGAATGTGACCTAATTTATGACAAATACGTAGAGCTCAGGTATCACTTCTAGTTTT60.18433179723502305No Hit
GTAATTATTAGGTCTAAGTAATACACAAAGTTAAACCCTTCACTATCTTAGTTGGAATATGTATAGAGGTA60.18433179723502305No Hit
GCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGATGATGACATGGGCTTTGGTCT60.18433179723502305No Hit
CTTGGTAAATACGGCATCATCTGCATGGAGGATTTGATTCATGAGATCTATACTGTTGGAAAACGCTTCAA60.18433179723502305No Hit
ACTACCGGAAGAAGAAACAGCTCATGAGGCTACGGAAACAGGCCGAGAAGAACGTGGAGAAGAAAATTGAC60.18433179723502305No Hit
GGACACTTAGCAAATGTAAAAATAAAATCTAACTCTCATTTGACAAGCAGAGAAAGAAAAGTTAAATACCA60.18433179723502305No Hit
GTATCAAGATAAAGGAATTCAAATAGCATATATATGACCATGTCTGAAATGTCAGTTCTCTAGCATAATTT50.15360983102918588No Hit
GTGAAACTACCAAAGGCTTTTGTGCTGTAGTAATCCTTTTCCTGTTTAAAACTCTGGGGATTTTCTAATGT50.15360983102918588No Hit
GGCGCTGTGCAGCCTCACCCCGGGCAGGGGCGGCCCTCGTTGTCAGGGCCTCTCCTCACTGCTGTTGTCAT50.15360983102918588No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT50.15360983102918588No Hit
GTGGGGTGGGACGACAGTGAAATCTAGAGTAAAATCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCA50.15360983102918588No Hit
GAACTAGAAAAAGCAGATTTAAGAACTGTAAAGAAATAATACTAGCTTTGAGTTTTTAAAAAAAATATTGA50.15360983102918588No Hit
GACCTACCCTGGAGTGGAAGCAGCTGGATTCTGTCCTCACGCTTCCTGTCTAAAGCCAGCTAGAGTAAATT50.15360983102918588No Hit
ACTGCACTCCAGCCTGGGTGACAGAGCAAAACTCCATCTCAAAAAACAAAAGAAGAAAAAAGAAAAGAATA50.15360983102918588No Hit
GTTTCAGGTGGTTTTAAAGAACACGTGGAAATCCCTTGAATTTAGGACCTGGTTAACCAGAAAGATAAGAC50.15360983102918588No Hit
GTAGTATTTTTTGTATAATTGGATTTCCTAATACTGTCATCCTCAAAGAAAGTGTATTGGTTTTTTAAAAA50.15360983102918588No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAG50.15360983102918588No Hit
GGGCCTAGGGAGCCGCACCTTGTCATGTACCATCAATAAAGTACCCTGTGCTCAATCAAAAAAAAAAAAAA50.15360983102918588No Hit
AGCATGGGCTGGGCCTGGGGCACAGGAGGAGCAGCTGCTTTGGTCGGGGTGGAGACTCGCAGCAGCTGCTA50.15360983102918588No Hit
AAGTTGGAAGATGAACACTTGAGGACTTCAGCTTCTCACCTACTTAGTACAGTTGGGAACCATACACTTCT50.15360983102918588No Hit
GGGGTGGAAGAAGTGGGGTGGGACGACAGTGAAATCTAGAGTAAAATCAAGCTGGCCCAAGGTGTCCTGCA50.15360983102918588No Hit
CTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTT50.15360983102918588No Hit
ACTTCCCTAACGTTTAATTGCCCTACAGATGCTTCTCTTGCTGTGGGTTTTCTTTTGTTAGTGGTCTGAAA50.15360983102918588No Hit
TCCTGTGGGTAGATCAGTGGATGAGACACTACGTTTGGTTCAAGCATTCCAGCACACTGACAAACACGGAG50.15360983102918588No Hit
GCTTAAACCTGATTTCTAAAAATTATCCCATTTTCTGATGATTTGAAGGGGAGAAAAGCCAGTGTATGTAA50.15360983102918588No Hit
GCCTTCAAGGAGACTCATGTAATGGATTACCGGGCCTTGGTGCATGAGCGAGATGAGGCAGCCTATGGGGA50.15360983102918588No Hit
CCCCTCCACCCCATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCTGCAGTTCCCTTTGGGTTCCAT50.15360983102918588No Hit
GTACTCTTCTCACTAATTTTTGTTGTGGTTAGTTAGAAAAATGGTTATTTTTAGTGGGTTTTAATATGTGA50.15360983102918588No Hit
GGAATTATGCATCATAAAGGAACCTAAGTGAGGTATATGATGAGTGTATTGTCTTTGCACACACATATAGG50.15360983102918588No Hit
CTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAACACATTT50.15360983102918588No Hit
GTTTTAAAATGTCAATCAATAGAGCAGTGGTTAAATAAACTACTCTTCAACCTGTAATATGGAATACTGTG50.15360983102918588No Hit
ACGTTGGGCAGCTGACCTTACTATCAGTGCCTCAGTTTCCTCCCCTGTAACCAGGGACAATAATGCCAGCC50.15360983102918588No Hit
GAAGGGGAGTAAAATTTCACTGTCTTATCTCACATAGAAGAGATTCTAAAATTATATTTAATTGTTGAATC50.15360983102918588No Hit
AGTCTAAAAGCCAACCCCCTCCCCACCCCAAGATGTTGTTTTGCCAAAGAAAATGTTTCTTTCAGATACAA40.1228878648233487No Hit
GCTGTCGCTGAGCTTCCTAGATAGAAACCAAAGCAGTGCAAGATTCAGTTCAAGGTCCTGAAAAAAGAAAA40.1228878648233487No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA40.1228878648233487No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGTAGCCAATGTTTCTCTTTTGGC40.1228878648233487No Hit
GGATGTTCCTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAA40.1228878648233487No Hit
CGCGGGGTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTAT40.1228878648233487No Hit
GCTTTTCTTGATAGGCCTAGTACAATCTTGGGAACAGGGTTACTGTATACTGAAGGTCTGACAGTAGCTCT40.1228878648233487No Hit
ATACAACTGTGTTTAATCTCCAAAGCCATATCCTCTCCTTCCATGAGGAAAGCCGCCCTTTTGTGTGTGAA40.1228878648233487No Hit
CCTTGACTTTGTACCATAACAATAACATAACTCAAAAGGATTTTGCTTTCTGAAGGCATACAAGTATCTGA40.1228878648233487No Hit
GCTTTTAATGTTGCGCCTCTTCAGGTTCTTAAGGGATTCTCCGTTTTGGTTCCATTTTGTACACGTTTGGA40.1228878648233487No Hit
GGGAGTTTGACTAAAGTTCTTTTTGTTTGTTTCAAATTTTCATTAATGTATATAGTGCAAAATACCATATT40.1228878648233487No Hit
GCCCAAGCTCAAGGCCTTCCTGGCCTCCCCTGAGTACGTGAACCTCCCCATCAATGGCAACGGGAAACAGT40.1228878648233487No Hit
GATTTTAATCTCTTACTTGTTAAAGTCTCATTTTAGTACCTTTGCATATATGATTCCATTCCTTTGTATAC40.1228878648233487No Hit
CCCTGGCCACTTCTTGTCTTTGCAACATTTTTTAGATATGTATTTGTGTTTTTTGGGGAGCTTAATTTGCT40.1228878648233487No Hit
CAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATACTTCACAACAATCCTAAT40.1228878648233487No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTA40.1228878648233487No Hit
TAGGACAACTGTTGCATGCCAAGTTTTTTGTGTGTGTGAAACACTTCAAAACTGATTTAAAAGATGTAAAT40.1228878648233487No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACACTATAG40.1228878648233487No Hit
CCTTTTGGATTATAAAAATATGGGGGAAAGTGCTATGATGAATTTTATGCAAAAAAAAAAAAAAAAAAAAA40.1228878648233487No Hit
GTTTCATGTTTTTGTATTATGTATCTTTTTGTATGGTAAAGACTATATTTTGTACTTAACCAGATATATTT40.1228878648233487No Hit
GTTTGGGGCTGCAGTGAGCTATGATGGTGCCTGTGAACAGCCACTGCATTCCAGCCTGGGCAGTGTGTTGA40.1228878648233487No Hit
GGTAAACCACCAATATCAACAGGTGATTTCAACAATCTCACTTGATATAAAGTCAATATACAAATTTAATT40.1228878648233487No Hit
GGTCTAAAATTGACACCCTAACATCACAATTAAAACAGCTAGAGAAACAAGAGCAAACAAATTCAAAAGCC40.1228878648233487No Hit
GGATCTCACTCTGTTGCTGAGGCTGGATCACAGCTCCCTGCAACCTTGAACTCTCCCTCAAGCAATTCTCC40.1228878648233487No Hit
CAAGTGATCCTCCCACTTCAGCCTTCCAAATAGGTGGGACTATTTTATTGCTCTTTGACCATTTTAATTGT40.1228878648233487No Hit
GTTATAATCTCAGTTGATAAATAAAAGACTAGGCACAGCTGAAAACAAATTAAGAAATTGGAAACAAAATT40.1228878648233487No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTT40.1228878648233487No Hit
TTCGTAGGATGGGTGAGTCAGATGATTCCATTCTCCGATTGGCCAAGGCCGATGGCATCGTCTCAAAGAAC40.1228878648233487No Hit
TTCTTCCTCAGCATAGTGCAGGACACGTCATGCTAATGAGGTCAAAAGAGAACGGGTTCCCTTAAGAGATG40.1228878648233487No Hit
CCTGTGTGATTTAAAAAGTTATAGCTTTTGCAACTTTATTACTGGTTATATACATTTGGCCATTATGATGT40.1228878648233487No Hit
CAGACTCCGTTTTCATTTTCTCGTGTTCTTTATGATAATGATCTTTGTAGATTGGTTATTTCTGTACTTTA40.1228878648233487No Hit
GGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1228878648233487No Hit
GGGTTAATGGCTAGTACTGTATTGGCTCTGTGAAAACATATTTGTGAAAAGAGTATGTAGTGGCTTCTTTT40.1228878648233487No Hit
CTCCTGGCTGTCAGTTTCTCAGTGGCAACTATTTACTGGTTGAAAATGGGAAGCAATAATATTCATCTGAC40.1228878648233487No Hit
CTCCCACCAGCTCTGCAGCCAGCCTATGGCAATTATATTTTAAGAGGTGTTCCCAGGACTTTTGGGACCTA40.1228878648233487No Hit
GAAGGGGAAAAATGATTGTTGTATACACTGAATTGCTTTGCATGGTCTCATTTGAGATAATTGATGTAAGA40.1228878648233487No Hit
TGACATCTTTAGATGCATTTCAGAGCCAATGATGATATTTGCTTTAGATAATTATTATATTATTATAAATA40.1228878648233487No Hit
CTATGAGGAGGGCCCTGGGAAGAATTTGCCATTTTTAGTGGAAAACAAGTGGTCGTTACTAGCTAAGATGT40.1228878648233487No Hit
GTCAGATACTGCAACAGGAGGAGAAAGTGCAGGCCATGCCACTTCCTCTCAGGAGCCTAGTGGATGCTCAG40.1228878648233487No Hit
CCATGTTTGCCTCAAGGGGAGGACACATCCAAGGCCTGACATCAGGTAAACGTAACAGAAGAGTCTTCAAG40.1228878648233487No Hit
GCCACCGCGGTAGCCGGGGCTGTGGCAGGGGCGGGCGCGGCCACCGGGACCGGCGTGGGAGCGACGCCAGC40.1228878648233487No Hit
GATTTGTTCTTGCAGATGTTCTTCTATCAAATACCTTATTTTACCTTACAGATATTTGTTGCACAGGCAGA40.1228878648233487No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCC40.1228878648233487No Hit
GTATTTATAAGTCAGTTGGCTCTAGGTTTTTTGTTCTTGTTTCTTTTTGTTTGGTTTTTGGACATTTAATG40.1228878648233487No Hit

[OK]Adapter Content

Adapter graph