FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002883379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002883379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length71
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAAAGGAGTAGAATACCAAATGATAGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGT1719.767441860465116No Hit
CTGTAAATGGTAATCAGTTGGAATTCTCCTAAATGTCTTCCAGACACTAGTAAAAAAAGACCTGAAAAAAA910.465116279069768No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG55.813953488372093No Hit
ACTGGAGGATTTATTTAAATAAAATATGCTTATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44.651162790697675No Hit
CTTTTGGAAGCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33.488372093023256No Hit
GAGCACCAAGACCTTGAACCTTTTTTGTTTTACCTTTTTTCCAAATAACAGTTGAAAAAAAAAAAAAAAAA22.3255813953488373No Hit
GAGCATATTTGCCAATAAAGCTTTTGGAAGCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22.3255813953488373No Hit
ATCCTATACATTAAAAGAAATTACACTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1627906976744187No Hit
GAAGAAGGCGGAGCCGCAGGAGAAGAGCACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGAGGAGTG11.1627906976744187No Hit
ATTTAAAGGAGTAGAATACCAAATGATAGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGT11.1627906976744187No Hit
CTTTTATAGAGCATATTTGCCAATAAAGCTTTTGGAAGCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1627906976744187No Hit
TTTTAAAGGAGTAGAATACCAAATGATAGCAACAGACTGCCTGAATTGAGACTTTTGATTTCTTAAAGTGT11.1627906976744187No Hit
GACCTACAAGCTTTAACACTTCTTGAAACTTAATGCACTACCGGAAAATCCTAACCATCTTCCTTGCTGAA11.1627906976744187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1627906976744187No Hit
GCTTTTTACTTTTCAGTCTGACGTTCTAATCGTCATGATTCGTGGTCGGCGTTGGCCCCGGAGCTACACGT11.1627906976744187No Hit
AAATAAAAAAAAAAAAAACAAAAACACTTAAAAAAAAACTATATATAAACCACACCATCAACCTAATAAAA11.1627906976744187No Hit
GACGAAATCGGAGACTCAGGAGAAGACCACCCTGCCGCCCTAAGAGGCCATTGCGCAGGAGACGCGGAGTG11.1627906976744187No Hit
TTTGTGACCTCTCCTCCCAACCCCCATTGCAGAATGCATAGGAACAATTCTCATTAAAAAATAAATAAGAG11.1627906976744187No Hit
GAAGAAAACGGAGACGCAGGAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAGTT11.1627906976744187No Hit
AATCACAGATTCTCAGACATGCCAACGATGCCTGTAACCTAAATCAGGAAGATCTAAGGAAACAAAAAGAC11.1627906976744187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1627906976744187No Hit
GAAGAAAGCGGAGACGCAGGAGAAGAACACCCTGCCGACCTAAGAGACGATTGAGCAGGAGAAGCGGAGTG11.1627906976744187No Hit
GAAGAAAACGGAGACGCAGGAGAAGAACACACTGCCGACCAAAGTGACCATTGAGCATGAGAAGCGGAGTG11.1627906976744187No Hit
ATACCTACACGACTCGTCCAATAAATCCATAATCAATTTAACTCACTACCTAAAGATATCTAAAGAGTAAT11.1627906976744187No Hit
GAAGAAAACGGAGACGCAGGAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAGTG11.1627906976744187No Hit
CTACAGAGGAGAAGAAGCCTGCTGCATAAACTCTTAAATTTGATTATTCCATAAAGGTCAAATCATATTGG11.1627906976744187No Hit
AAATTAGACAATAAAGGAAACAGAAATAAATAAATATACACAACTCTAAATCAAAAATAAAAAATAAAAAA11.1627906976744187No Hit
GTGAAAAGATTGACCTGCACGCGGAGACGGTTGCCCCGTCCACATTCGTCCGGCCCCTCTAGACCTCCATA11.1627906976744187No Hit
TTTTAAAGGAGTAGAAAACCAAATGATAGCAACAGACTGCCTGAATTGAGAATTTAGATTTCTTAAAGTGT11.1627906976744187No Hit
GTCTGAGGCAAGATGGAGGGTATGAGGGGCCTTCACACTACACATCATCCCTTCTACCCATCACAACATAC11.1627906976744187No Hit
TTTTAAAGGAGTAGAATACCAAATGATAGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTAAAAGTCT11.1627906976744187No Hit
CCTCTCTCCCCCTGGGCCACTCCCGGGGGTGAGGGGGTTACCCCTTCCCAGTGTTTTTTATTCCTGTGGGG11.1627906976744187No Hit
CTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG11.1627906976744187No Hit
CTGTAAATGGTAATCAGTTGGAATTCTCCTAAATGTCTTCCAGACACTAGTAAAAAAAGCCATAAAAAAAA11.1627906976744187No Hit
GGAAAAAAGACCCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGG11.1627906976744187No Hit
GCCGATTACTCGTTACATGTGTCGAAATTGTCAACGACTCACAAACTACGCTTCATTCGATAAACAGGAGC11.1627906976744187No Hit
GATTTATATATTGATTTTTTTTTCTACTCTCAAACTTTAATCTAAATAAAATCTTTTTAAAATGAGAAAAA11.1627906976744187No Hit
ACATTATAGAAAAAAAAAGCACCAAAATAATAAGAATTTTAAAAAATGCAAAAAAATAAGAACAAAATTTT11.1627906976744187No Hit
CTGTAAATGGTAATCAGTAGGAATTCTCCTAAATGTCTTCCAGACACTAGTAAAAAAAGACCTGAAAAAAA11.1627906976744187No Hit
GAGCATATTTGCCAATAAAGCTTTTGGAAGCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1627906976744187No Hit
GGAAAAAAGACCCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAATAAAAAGGGGAAAAGAAGGGAAA11.1627906976744187No Hit
CAAAACATCCAATATCAAAGAAAAATCTAAAACTTAAATCTTCTATATAATATACTAGAAAAAACTAAAAC11.1627906976744187No Hit
GTGTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATGGTTGAAATAGGGCCCGTATTTACCGTATTG11.1627906976744187No Hit
GAAGAAAACGGAGACGCAGGAGAAGCACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAGTG11.1627906976744187No Hit
TTTTAAAGGAGTAGAATACCAAATGATAGAAACAGCCTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGT11.1627906976744187No Hit
TTTTCATGCACATCGTACTAGAATTTATTCCCCTTAAAATCTTTGTAATAGGTCACGTATTTAACATATAA11.1627906976744187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGAGGGAGAGACAAAAAAAAAAAAAAA11.1627906976744187No Hit
CCACCAACACACTGAATCTCCCCTCCTCACAGATTCCATGTAGAACCCTTGAAGAGGGGAGGGGCCTAGAG11.1627906976744187No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCTTATTTACACTATAG11.1627906976744187No Hit
CCATATACCCAATCTTGGCACAAAAAATTAAAATAAATAAAGATCAAGATATTGATACGATATCAAGCTGC11.1627906976744187No Hit
AAGACAAGCTAAGAAACAAAAAGCTAAGTCCAAAAACTACAAACTAACAAGAAAAATCCAACATACAAACA11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)