FastQCFastQC Report
Mon 8 May 2023
EGAF00003029881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003029881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24826003
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTG1182610.476359404290735TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGA439230.17692336539232675TruSeq Adapter, Index 20 (97% over 43bp)
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAA374310.1507733645242853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)