FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003030397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003030397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250533
Sequences flagged as poor quality0
Sequence length209-223
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT141975.666718556038526No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC117994.7095592197435066No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT104684.178291881708198No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG102844.104848463076721No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG95013.7923147848786387No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT91423.649020288744397No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG82613.2973700071447674No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG74302.9656771762602134No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT70292.805618421525308No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT70282.805219272511007No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT70272.804820123496705No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG64272.5653307149157993No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG59942.392499191723246No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT59712.3833187643943115No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT55552.2172727744448837No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC55082.198512770772713No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA51912.071982533239134No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG51352.0496301884382495No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG50302.0077195419365914No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG49061.9582250641632042No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA46461.8544463204448116No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG45081.7993637564712035No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA43281.7275169338969318No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG43161.7227271457253137No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC38601.5407151952038254No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA38311.5291398737890818No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT37291.4884266743303276No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA36801.468868372629554No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC36231.4461168788143677No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT34291.3686819700398751No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT29931.1946529998044169No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA28121.1224070282158438No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG27671.104445322572276No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA26481.0569465898703965No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC25901.0337959470409088No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT24460.9763184889814914No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC24040.9595542303808281No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT22970.9168452858505667No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT20140.8038861148032395No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC20050.8002937736745258No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT19290.7699584485876112No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC16620.6633856617691083No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG16270.6494154462685554No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG15250.6087022468098016No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC14800.5907405411662336No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG13910.5552162788933993No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA10150.40513624951603183No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT6910.27581196888234283No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT3280.130920876690895No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT2690.10737108484710597No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT2530.10098470061828183No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT2530.10098470061828183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)