FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003030399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003030399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244
Sequences flagged as poor quality0
Sequence length211-220
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC135.327868852459016No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG135.327868852459016No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT114.508196721311475No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG83.278688524590164No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG83.278688524590164No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG83.278688524590164No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA72.8688524590163933No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT62.459016393442623No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT62.459016393442623No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG62.459016393442623No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG62.459016393442623No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG62.459016393442623No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT52.0491803278688523No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT52.0491803278688523No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG52.0491803278688523No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT52.0491803278688523No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC52.0491803278688523No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG52.0491803278688523No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA52.0491803278688523No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA41.639344262295082No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC41.639344262295082No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC41.639344262295082No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT31.2295081967213115No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC31.2295081967213115No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG31.2295081967213115No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT31.2295081967213115No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA31.2295081967213115No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT31.2295081967213115No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT31.2295081967213115No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG31.2295081967213115No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT20.819672131147541No Hit
GAGGGTGAACTCACTGGAATGGGGATGCTTGCATGTGTAATCTTACTAAG20.819672131147541No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT20.819672131147541No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC20.819672131147541No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG20.819672131147541No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC20.819672131147541No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA20.819672131147541No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT20.819672131147541No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA20.819672131147541No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT20.819672131147541No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA20.819672131147541No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC20.819672131147541No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA10.4098360655737705No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA10.4098360655737705No Hit
AAGTATACTTGCGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT10.4098360655737705No Hit
ACCTACGAGTACCCCGACTACGCCGGACCAATCTTCAACCTCCACACACT10.4098360655737705No Hit
GAGGAGTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA10.4098360655737705No Hit
TAATCCTTCTCACCCTAACAGGTCGACCTCGCTTCCCCACCCTTACTAAC10.4098360655737705No Hit
TAGGATTGGTGCTGTGGGTGTAAGAGTATGATGGGGCGGTGGTTGTGGTA10.4098360655737705No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG10.4098360655737705No Hit
ACGACCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA10.4098360655737705No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCTCTCTAGCTATCATTCT10.4098360655737705No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCACCTGGTTCTAG10.4098360655737705No Hit
CGTAACCTCAACACCACCTTCTTCGACCCAGCCGGAGGAGGAGACCCCAT10.4098360655737705No Hit
AAGCATTGGACTGTAAATCTCAAGACATGGGTTAGGCCTCTTTTTACCAG10.4098360655737705No Hit
AACTCCTGAGCAAACAAATTAATATGACTACCTTCCACAATACCTTTTAT10.4098360655737705No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTACG10.4098360655737705No Hit
CGCAACCTCAACTAAACCTTCTTCGACCCCCCCGGAGGAGGAGACCCTAT10.4098360655737705No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG10.4098360655737705No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTGCGCACCGACCTACTGTAG10.4098360655737705No Hit
TTGGAGTTGAGCTGTTGGGAGGTGGGGATGAATAGAGGGGGAAATAGAAT10.4098360655737705No Hit
CATTTTCGGGGTATCGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG10.4098360655737705No Hit
AATGCTTCACTCAGCCATTTTACCTCACTCCCACTGATGTTCGCCGACCG10.4098360655737705No Hit
AAGAATTGGACGGTAAATCTAAAGACAGGGGTTAGGCCGCTTTTTACCAG10.4098360655737705No Hit
TCTCCGTGCCACCTATCACACCACCTCCTAAAGTAAGGTCAGCTAAATAA10.4098360655737705No Hit
TAATTCTTCTCACCATAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC10.4098360655737705No Hit
GGACATAGCATATGAAGGCTGTTGCTATAGTTGAAAACAGGAGGTAAATG10.4098360655737705No Hit
GGTTGACCTGTGAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT10.4098360655737705No Hit
CATCGCCACTTATCCAGTGAACCACGTACGCACCGACCTACTGTAGATGA10.4098360655737705No Hit
CAAGCAATCCTATACAACAGTAACGGCGATATCGGTTTCATCCTCGCCTT10.4098360655737705No Hit
AAGTATACTTGAGGCGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT10.4098360655737705No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT10.4098360655737705No Hit
CGCGAGCTAAACACCACCTTCTTCGATCCCGCCGGAGGAGGAGACCCCAT10.4098360655737705No Hit
GGTCGACCTGTTAGGGTGAGAAGGATTATTCGAGTGCTATAGGCGCTTGT10.4098360655737705No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCAAGGAGTCAATAAAGTGATTGGC10.4098360655737705No Hit
GAAGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG10.4098360655737705No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTATCTTGTTCCGTTGGTCAAG10.4098360655737705No Hit
AAAAAATACCAAACATATAACTGTACTTCACACACCCAATTGGACCAATC10.4098360655737705No Hit
TGTGCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA10.4098360655737705No Hit
TCGCCGATAAGGTTGTATAGGATTGCTTGAATGGCTGCTGCGTTAGCATC10.4098360655737705No Hit
AAGTATGCTTGAGGACGGTGACGGGCGGTGCGTACGCTCTTCTGCGTCCT10.4098360655737705No Hit
TCTCCGTGCCACCAATCACACACCATCCTAAAGAAAGGTCAGCTAAATCA10.4098360655737705No Hit
AAAAAATCCCAAACATATAACTGAACTCCTAACACCCAATTGGACCAATC10.4098360655737705No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAGCTAAAACCCCTACGCATTT10.4098360655737705No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTCAGGCCTCTTTTTACTAG10.4098360655737705No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATTATTCT10.4098360655737705No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG10.4098360655737705No Hit
AAGTATACTTGTGCAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT10.4098360655737705No Hit
GTGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATATTAGTAAG10.4098360655737705No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCTCCTCTAT10.4098360655737705No Hit
GTAAAGCCTAAATCGCCCACACGTTCCCCTTAAATAAGACATAACGATGG10.4098360655737705No Hit
GGTTGACCTGTTAGGGTGAGAGGAAGTATTCGAGTGCTATAGGCGCTTGT10.4098360655737705No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG10.4098360655737705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)