FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003030838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003030838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161550
Sequences flagged as poor quality0
Sequence length210-222
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG81365.036211699164346No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC74334.6010523057876815No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT64864.01485608170845No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG61903.831631073970907No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT54423.3686165273909006No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG54393.3667595171773446No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT53853.3333333333333335No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG51613.194676570721139No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT47422.9353141442277932No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT46732.8926029093160013No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG43332.6821417517796347No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT43242.676570721138966No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA36012.229031259671928No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA34382.128133704735376No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG33912.0990405447229956No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG33472.0718043949241722No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT33432.0693283813060974No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT33352.0643763540699474No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG32972.040854224698236No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA29621.833488084184463No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG29111.801918910554008No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA28791.782110801609409No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC27061.6750232126276694No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG26301.6279789538842464No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG25701.5908387496131229No Hit
ACTCCCTCTTAGCCAATATTGTACCTATTGCCATACTAGTCTTTGCCGCC24081.4905601980810896No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT23161.4336118848653667No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA22841.4138037759207676No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA22471.3909006499535748No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC21511.331476323119777No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC21501.3308573197152584No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA20911.2943361188486535No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT20191.2497678737233053No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG18651.154441349427422No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC17521.084493964716806No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT17091.0578768183225007No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA16641.030021665119158No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC15990.9897864438254411No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT14840.9186010523057876No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC14130.8746518105849582No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT13720.8492726709996906No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT13480.8344165892912412No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG13120.812132466728567No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC10530.6518105849582173No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT9040.5595790776849273No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT8720.539770968740328No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG4770.29526462395543174No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT4520.27978953884246366No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT3870.23955431754874654No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT3670.227174249458372No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT3390.2098421541318477No Hit
GGCTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT2420.1497988238935314No Hit
CGTAACCTCAACACCACCTTCTTCGACCCAGCCGGAGGAGGAGACCCCAT2120.13122872175796968No Hit
TCAACTTAAACTCCAGCACCACAACCCTACTACTATCTCGCACCTGAAAC1870.11575363664500156No Hit
GCGAAGGCTTCTCAAATTATGAAAATTATTAATATTACTGCTGTTAGAGA1650.10213556174558959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)