FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003030846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003030846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135699
Sequences flagged as poor quality0
Sequence length209-222
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT57504.237319361233318No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG54754.0346649570004205No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG53053.9093876889291743No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC52093.8386428787242357No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG43893.2343642915570494No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT40813.0073913588162036No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG39442.9064326192529055No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT37432.7583106728863145No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT32612.4031127716490173No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG32252.376583467822165No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT31812.3441587631449012No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT30652.2586754508139335No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT29432.1687705878451573No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA28302.085498050833094No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT27792.04791487041172No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG26771.9727485095689727No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG26531.9550623070177378No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA25131.8518927921355355No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA25121.851155867029234No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC24701.820205012564573No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG24121.777463356399089No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG24031.7708310304423762No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG22911.6882954185366141No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG22681.671346141091681No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA22531.6602922644971592No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA21171.5600704500401625No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC20651.5217503445124871No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG20451.5070118423864582No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC19761.4561640100516586No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC19031.402368477291653No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT17761.3087789887913692No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT17421.28372353517712No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG17171.2653004075195837No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG16811.2387711036927316No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA15111.1134938356214859No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG14341.0567506024362743No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC14281.0523290517984658No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA13921.0257997479716137No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT13721.0110612458455848No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA13270.9778996160620196No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC13140.9683195896801009No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT12220.9005224799003677No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT11990.8835732024554346No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC11940.8798885769239272No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT11170.823145343738716No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA9830.7243973794943219No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC9270.683129573541441No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC9040.6661802960965078No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG8410.6197540143995166No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT7210.531323001643343No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT5990.4414181386745665No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT3890.28666386635126273No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT3880.28592694124496126No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT3640.26824073869372655No Hit
ATTAAGTTATTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTAAGAGTGAG2630.19381130295728044No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT2440.17980972593755298No Hit
GGCTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT2190.1613865982800168No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG2060.151806571898098No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG1890.1392788450909734No Hit
TATTTTTGGGCTGTTGCTTTTTACTTGTCATTTTTGTGTTGTAAACCCTT1850.13633114466576762No Hit
ATTAAGTTACTAGCTCAGGAGTTTGATAGCTCTTGGGCAGTAAGGGTGAG1820.13412036934686328No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG1680.12380341785864302No Hit
GTGCAAGGAGTTCAGTTATGTGTTTGGGATTTTTTAGATGACAGGTGTCG1660.12232956764604012No Hit
CCTCCTCAAGTATACTTCAAAGGACACTTAACTAAAACCCCTACGCATTT1470.10832799062631265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)