FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003031100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419165
Sequences flagged as poor quality0
Sequence length208-223
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT253986.059189102143547No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG237245.659823697112116No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG215075.130915033459377No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG193364.612980568511206No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT192174.584590793601565No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC178424.256557680149822No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG161263.847172354562046No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG160343.825223957152911No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT148493.5425190557417725No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT130663.1171495711712573No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT129273.083988405520499No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT114862.740209702623072No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT112402.6815215965073422No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA109702.6171078215022723No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA98862.3584984433337706No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG97532.3267686949053474No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA94972.265694893419059No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG83321.9877613827490366No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG81271.938854627652595No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG77011.8372240048668185No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT76651.8286355015328093No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC75681.8054942564383953No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA74671.7813987331957584No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA72531.7303448522658142No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA69571.6597282692972934No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG63281.5096680304891867No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC61391.4645783879856382No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT58231.389190414276001No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG56661.3517349969582384No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA52541.2534443476912434No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT48131.1482351818496297No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG47271.1277182016628295No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT41991.0017534860973603No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT39300.9375782806293466No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC39020.9308983335917836No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC37420.8927272076628535No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC36940.8812758698841745No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA33870.8080350220080399No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT29080.6937602137583052No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC23810.5680340677298915No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC23140.552049908747152No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT16220.3869597891045293No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG12160.29010055705986904No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT11510.2745935371512412No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC8340.1989669939045483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)