FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003031244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length211-220
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT67.894736842105263No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT45.263157894736842No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA45.263157894736842No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG45.263157894736842No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT33.9473684210526314No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT33.9473684210526314No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC33.9473684210526314No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT22.631578947368421No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA22.631578947368421No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA22.631578947368421No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC22.631578947368421No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT22.631578947368421No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT22.631578947368421No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC22.631578947368421No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG22.631578947368421No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA11.3157894736842104No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG11.3157894736842104No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA11.3157894736842104No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG11.3157894736842104No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCAAGGAGTCAATAAAGTGATTGGC11.3157894736842104No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC11.3157894736842104No Hit
CGGCCGCTACGGAAGCATAGGGTTGCGTGAATGGCTGCGGTGATCGCATC11.3157894736842104No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA11.3157894736842104No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG11.3157894736842104No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT11.3157894736842104No Hit
GAGGGTGAACTCACCGGAATGGGGATGCTTGCATGTGTAATCTTACTAAG11.3157894736842104No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC11.3157894736842104No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGAGTTGGTATAGAATG11.3157894736842104No Hit
TATAACTTTCAGGGTGACCGAAAAATACGAATAGATGCAGGAATAGAATT11.3157894736842104No Hit
TCGCCGATACGGTTGTATGGGATTGCTTGAATGGCTGCTGTGTTGGCATC11.3157894736842104No Hit
CATTTTCGGGGTATGGGGCCGATAGCTTATTTAGCTGACCTTACTTTAGG11.3157894736842104No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT11.3157894736842104No Hit
TAGGATTGGCGCTGTGGTTGAAAGAGTATGATGGGGTGGTGGTTGGGGTA11.3157894736842104No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG11.3157894736842104No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG11.3157894736842104No Hit
GGCTGACCAGTTTGGGTGAGAAGAATCATTAGAGTGCTATAGGCGCTTGT11.3157894736842104No Hit
TATGGAGTGGAAGTGAAATCATGGCTAGGCCGGAGGTCATTAGGAGGGCT11.3157894736842104No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG11.3157894736842104No Hit
CCTCCTCAAATATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT11.3157894736842104No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT11.3157894736842104No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA11.3157894736842104No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG11.3157894736842104No Hit
TATGAGTGTGTTTTGTCACCGCCCAGCATAACGCAAGGTCAGCTGAAGTT11.3157894736842104No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGCTGTTCGCCGACCG11.3157894736842104No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGATTGT11.3157894736842104No Hit
ATAAAGCCTAAATAGCACACACGTTCCCCTTAAATAAGACATCACGATGG11.3157894736842104No Hit
GAGCGTGAACTCACGGGAATGGGGATGCCTGCATGAGTGATCTTACTAAG11.3157894736842104No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCCACTGATGTTCGCCGACC11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)