FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003031256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length211-220
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT88.16326530612245No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG77.142857142857142No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT55.1020408163265305No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA55.1020408163265305No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT44.081632653061225No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG44.081632653061225No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT44.081632653061225No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT44.081632653061225No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT33.061224489795918No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG33.061224489795918No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC22.0408163265306123No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT22.0408163265306123No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG22.0408163265306123No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC22.0408163265306123No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG22.0408163265306123No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT22.0408163265306123No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC22.0408163265306123No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG22.0408163265306123No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT22.0408163265306123No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG22.0408163265306123No Hit
GGTTGACATGCTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT11.0204081632653061No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA11.0204081632653061No Hit
ACCTACGAATACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT11.0204081632653061No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC11.0204081632653061No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC11.0204081632653061No Hit
AATATTAGGCATACCCTCCCCAGTTTTTCCCCACATATTTCCCCCTCTAC11.0204081632653061No Hit
AATGCTTCACTCAGCCATTGTACCTCACCCCCACTGATGTTCGACGACCG11.0204081632653061No Hit
TAGGGAGTGGAGGTGAGATCACATGGCTAGGCCGGAGGTCAGTTGGGGGG11.0204081632653061No Hit
AATACTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT11.0204081632653061No Hit
GCCTAGCCATGCGATTTCACTTCCACTCCATAACGCTCCTCACACTAGGC11.0204081632653061No Hit
TCGGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC11.0204081632653061No Hit
AATGCTTCACTCAGCCATTTTACCTCACTCCCACTGATGTTCGCCGACCG11.0204081632653061No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT11.0204081632653061No Hit
CCTCCTCCAGTATACTTCCAATGACATTTAACTAAAACCCCTACGCATCT11.0204081632653061No Hit
AGTACTACTCGATTGACAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG11.0204081632653061No Hit
GGTCATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG11.0204081632653061No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG11.0204081632653061No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGTGTGTGTGCCTGCAAAGCCGG11.0204081632653061No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT11.0204081632653061No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGTGGAGACCCCAT11.0204081632653061No Hit
GGACATAGCTTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG11.0204081632653061No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG11.0204081632653061No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT11.0204081632653061No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCAT11.0204081632653061No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG11.0204081632653061No Hit
GCGAAGGCTCCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA11.0204081632653061No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC11.0204081632653061No Hit
AGAAAATCCCAAACATATAAGTGAATTTATCACCACCCATTGGAATAATG11.0204081632653061No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG11.0204081632653061No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC11.0204081632653061No Hit
TGAATCGGAGGATAACCAGTAAGCTACGCTTTTACCATAATAGGACAAGT11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)