FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003031265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133703
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT101377.581729654532808No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA72365.411995243188261No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA56604.233263277562957No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC56174.201102443475464No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA54974.111351278580137No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT48633.6371659573831554No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA48023.5915424485613636No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA44933.360433198955895No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA40773.0492958273187587No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC37842.8301533996993333No Hit
TCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT35012.618490235821186No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT34172.5556644203944563No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC30182.257241797117492No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC29202.1839450124529742No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT27802.079235320075092No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT27792.0784873937009642No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA27022.0208970628931286No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA26461.979013185941976No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA26421.976021480445465No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG24691.8466302177213674No Hit
GGAGAACCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG23521.7591228319484231No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT21951.6416983912103693No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT20411.5265177295946988No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA19261.4405061965700097No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT18151.3574863690418315No Hit
CGCCCACGGACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT18121.3552425899194482No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC15921.1906987876113475No Hit
CAGGTGGTCAAGTATTTATGGTACCGTGCAATATTCATGGTGGCTGGCAG15861.1862112293665812No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT15541.1622775853944938No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA14651.0957121380971258No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG14031.0493407029012063No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA13220.9887586665968602No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG12900.9648250226247728No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT12410.9281766302925141No Hit
TTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG11890.889284458837872No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT11840.8855448269672334No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC10920.8167356005474821No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT10720.8017770730649274No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA9560.7150176136661107No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA9170.6858484850751292No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC8460.6327457125120604No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT8390.6275102278931662No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT8320.6222747432742721No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC7710.5766512344524805No Hit
GATGATTAGTTCTTCTATGATAGGAGAAGTAGCGTCTTGTAGACCTACTT5580.41734291676327384No Hit
AGGGGCAGGTTTTGGCTCGTGAGAAGGCCTAGATAGGGGATTGTGCGGTG5540.4143512112667629No Hit
TTTTAGCAAAATAGTAACTATTTTTGCTTAAATGAGATTGCACATGGATT4960.3709714815673545No Hit
AATAAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC4400.32908760461620157No Hit
AACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT4100.3066498133923697No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTGGTATTGGTTATGGTTC3540.2647659364412167No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGTTTGGTGGAAATT3250.24307607159151254No Hit
GGCGATTAGCTCTTCTATGATAGGGGATGTGGCGTCTTGAAGACCTAGCT3120.23335302872785202No Hit
TATTTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT2880.21540279574878649No Hit
AGAGAACCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG2600.19446085727321002No Hit
GACCCCCCTTCTAATACGGACAACCTGACTCCTACCCCTCATAATCATAG2500.18698159353193272No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA2480.18548574078367724No Hit
GACCCCCCTCCTAATACTAACTACCTGACTCCTGCCCCTCACAATCATGG2400.1795023297906554No Hit
TCAGCCCCATTTTATTTTTTTAATTTATTTTTGAGACAGTGTCTCACTCT2300.17202306604937811No Hit
CAGTGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTGT2110.1578124649409512No Hit
GCCGGCCTCCACTATAGCAGATGCAAGCAGGAGTAGGAGAGAGGGGGGTA2090.15631661219269574No Hit
TGCGTTTAACGGCCGCGGTACCCTAACTGTGCAAAGGTAGCATAATCACT2030.15182905394792937No Hit
GGCCTTCTCCACTTCAAGCCAACTAGGACTTATAGTAGTCACAATCGGCA1990.14883734845141844No Hit
TAACCAATACCACCAATCAATACTCATCATTAATAATCATAATGGCTATA1800.13462674734299157No Hit
CCCTTATCCCTATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA1760.13163504184648064No Hit
GGCCTTCTCCACTTCAAGCCAACTAGGACTTATAATAGTCACAATCGGCA1630.12191199898282012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)