FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003031312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183883
Sequences flagged as poor quality0
Sequence length208-223
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG171319.316250006797802No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC64993.534312579194379No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT55313.0078908871401926No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT53792.9252296297101963No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC47932.606548729355079No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG45602.4798377228998874No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC44792.435787973874692No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC44582.424367668571864No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT43972.3911944007874575No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC43462.3634593736234453No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA41402.251431616843319No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG40782.217714524996873No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT40222.1872603775226636No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG39712.159525350358652No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT36701.9958343076847778No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG34981.9022965690139928No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG33731.8343185612590616No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT32241.7532887760151834No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT31161.6945557773149231No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA29871.6244024733118343No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG28721.5618627061772976No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG27301.4846396893676959No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC26831.4590799584518417No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG26071.4177493297368435No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC25101.364998395719017No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG24771.3470522016717152No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC24231.3176857023215849No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA22991.2502515186286933No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT22681.2333929727054704No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA21701.1800982146256043No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA21561.1724846777570521No Hit
TCAACTTAAACTCCAGCACCACGACCCTATTACTATCTCGCACCTGAAAC19101.0387039584953475No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG18421.0017239222766652No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT18260.9930227372840338No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT17870.9718135988644954No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT17770.9663753582441009No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG16320.8875208692483807No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT16320.8875208692483807No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG15550.8456464164713432No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA14820.8059472599424635No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA13910.7564592702968735No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA13660.7428636687458874No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA13160.7156724656439148No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT12540.6819553737974691No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT12180.6623777075640489No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG9330.5073878498828059No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAACT8820.479652822718794No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT8300.45137397149274266No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG7570.4116748149638629No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC7330.39862303747491606No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG6840.3719756584349831No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG6260.3404338628366951No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT6210.3377147425264978No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG5720.31106736348656483No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC5560.30236617849393366No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG5240.28496380850867126No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA4820.2621231979030144No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG4050.22024874512597686No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC3170.17239222766650533No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG3140.170760755480387No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT3120.16967310735630808No Hit
ACGACCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA3030.16477869079795304No Hit
TTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCA2850.15498985768124296No Hit
GGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTG2340.12725483051723108No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT2340.12725483051723108No Hit
TTACCTCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA2070.11257158084216595No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT2030.11039628459400815No Hit
TCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGAC2000.10876481240788981No Hit
TCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAA1970.10713334022177144No Hit
TCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACG1870.10169509960137697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)