FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003031738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225159
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT115165.114607899306712No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA93424.149067991952354No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT89753.9860720646298837No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA86003.8195230925701393No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA83353.70182848564792No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT75773.3651774967911567No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA67272.9876664934557358No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC66092.9352590835809362No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA66032.93259430002798No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT61022.7100848733561618No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA58752.6092672289359964No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT55242.4533773910880754No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA53632.381872365750425No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC52602.3361269147580153No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA51182.2730603706713923No Hit
TTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG50812.2566275387614976No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC49302.1895638193454405No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA45772.0327857203132007No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA43341.9248619864184864No Hit
TCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT41581.8466950021984465No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA40941.8182706443002499No Hit
GGAGAACCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG40701.8076115100884262No Hit
CAGGTGGTCAAGTATTTATGGTACTGTGCAATATTCATGGTGGCTGGCAG38341.7027966903388272No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT38281.7001319067858713No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT38211.697022992640756No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG37731.675704724217109No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGTATTGTATTAGCAAACT37651.6721516794798343No Hit
CGCCCACGGACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT37631.6712634182955155No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA37531.6668221123739224No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT37091.6472803663189124No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC35001.5544570725576148No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA34851.547795113675225No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA32641.4496422528080155No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC32041.4229944172784565No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT31111.3816902722076398No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT30011.332835907070115No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA27831.2360154379793835No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA26711.1862728116575398No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC25081.1138795251355709No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG23911.0619162458529305No Hit
CCCCCTCTACCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTA22751.0103970971624496No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG18760.8331889908908815No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC15650.6950643767293335No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT13300.5906936875718937No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGTTTGGTGGAAATT12770.5671547661874498No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT11290.5014234385478706No Hit
GATGATTAGTTCTTCTATGATAGGAGAAGTAGCGTCTTGTAGACCTACTT10190.4525690734103456No Hit
GGCGATTAGCTCTTCTATGATAGGGGATGTGGCGTCTTGAAGACCTAGCT8470.3761786115589428No Hit
TTTTAGCAAAATAGTAACTATTTTTGCTTAAATGAGATTGCACATGGATT6920.30733836977424844No Hit
AATATTTTTTTATTATAGGAAATGGCTGAGTGTGGTGGCTCTGTAATCCC4730.2100737700913577No Hit
TCAGCCCCATTTTATTTTTTTAATTTATTTTTGAGACAGTGTCTCACTCT4620.20518833357760516No Hit
AGAGAACCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG3390.15056027074200898No Hit
AGGGGGTAGAGGGGGTGCTATAGGGTAAACACGGGCCCTATTTCAAAGAT2950.13101852468699896No Hit
AGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGAT2940.13057439409483965No Hit
TTCTCTTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTACAGG2500.11103264803982962No Hit
GACCCCCCTTCTAATACGGACAACCTGACTCCTACCCCTCATAATCATAG2430.10792373389471441No Hit
GACCCCCCTCCTAATACTAACTACCTGACTCCTGCCCCTCACAATCATGG2370.1052589503417585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)