FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003031753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67081
Sequences flagged as poor quality0
Sequence length208-223
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT60489.015965772722529No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC33985.065517806830548No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC26934.014549574395134No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC25983.8729297416556103No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT24113.5941622814209686No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC19852.9591091367153144No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA19302.8771187072345374No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC18552.7653135761243868No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG16542.4656758247491837No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG15652.333000402498472No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG14142.1078994051967026No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT13171.9632981022942413No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG13081.9498814865610232No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG12711.8947242885466824No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG12131.8082616538214993No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT12091.8022987134956248No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT11791.7575766610515644No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT11711.7456507803998151No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG11671.7396878400739404No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC11661.7381971049924716No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG11621.7322341646665973No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT10871.6204290335564469No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG10181.5175683129351083No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT10101.5056424322833588No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT9521.419179797558176No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA9411.4027817116620207No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC9281.383402155602928No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG8791.3103561366109628No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC7891.1761899792787824No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT7871.173208509115845No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT7651.1404123373235342No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA7191.0718385235759753No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG7071.0539497025983513No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG6550.9764314783619802No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT6210.9257464855920454No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA6090.9078576646144214No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG5990.8929503137997347No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG5960.8884781085553286No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA5340.796052533504271No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA5190.7736915072822409No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT4890.7289694548381808No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG4870.7259879846752433No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA4850.723006514512306No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC4460.6648678463350278No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCATGC4320.6439975551944664No Hit
TCAACTTAAACTCCAGCACCACAACCCTACTACTATCTCGCACCTGAAAC4220.6290902043797797No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT3850.5739330063654389No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA3710.5530627152248774No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT3030.45169272968500773No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA2920.4352946437888523No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG1880.28025819531611035No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG1770.26386010941995497No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC1710.25491569893114296No Hit
TTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCA1230.18336041502064668No Hit
TCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAT1040.1550364484727419No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT1030.15354571339127324No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT910.13565689241364917No Hit
GCGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTG880.13118468716924314No Hit
GGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTG850.12671248192483714No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT730.10882366094721307No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT710.10584219078427573No Hit
CATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGAC700.10435145570280706No Hit
TTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCATGCT690.10286072062133837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)