FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003031967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003031967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93321
Sequences flagged as poor quality0
Sequence length209-223
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT62376.68338316134632No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC58646.283687487275104No Hit
CGAGCAGTAGCCCAGACAATCTCATATGAAGTCACCCTAGCCATCATTCT45334.857427588645642No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG40284.316284651900429No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC33573.5972610666409492No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT32463.4783167775741792No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT32343.465457935512907No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG31183.3411557955872744No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG28933.100052506938417No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA28213.0228994545707826No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT28123.0132553230248282No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG27842.9832513582151927No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT27762.9746787968410113No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT26052.79144029746788No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT24822.659637166339838No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG24692.6457067541067927No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC23662.535335026414205No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT22152.3735279304765275No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG21952.3520965270410734No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC20552.2020767029928954No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA18091.9384704407368119No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA18001.9288263091908575No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG17911.9191821776449032No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC17651.891321353178813No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG16991.820597721841815No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG15451.6555759153888194No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA15371.6470033540146378No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA15291.6384307926404562No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT14771.5827091437082756No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT13471.443405021377825No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC12611.3512499866053729No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG7230.7747452341916611No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG7180.7693873833327975No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC5790.6204391294563925No Hit
TATGAGATTGTCTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT5720.6129381382539836No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA5250.5625743401806668No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC4050.4339859195679429No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT3820.40933980561717087No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC2410.2582484113972204No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG1930.20681304315213084No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA1570.16823651696831368No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT1390.1489482538764051No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT1200.12858842061272382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)