FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003032149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003032149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33335
Sequences flagged as poor quality0
Sequence length209-223
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT28218.462576871156442No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC22716.812659367031648No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC14984.4937753112344385No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC12273.68081595920204No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC10673.2008399580020996No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG10153.0448477576121196No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT9022.705864706764662No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC7392.2168891555422228No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG7342.2018899055047245No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT7102.1298935053247336No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT7092.126893655317234No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG6832.048897555122244No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG6802.039898005099745No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG6331.8989050547472626No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG5981.793910304484776No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT5961.7879106044697766No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA5911.7729113544322783No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG5651.694915254237288No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC5361.6079196040197992No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT5341.6019199040047998No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG5101.529923503824809No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA5071.52092395380231No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT4931.4789260536973152No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC4541.3619319034048298No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC4021.2059397030148493No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT3881.1639418029098545No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA3601.079946002699865No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG3601.079946002699865No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT3521.055947202639868No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT3371.0109494525273737No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT3170.9509524523773811No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA3130.9389530523473827No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG3110.9329533523323833No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG2880.8639568021598921No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA2700.8099595020248987No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG2650.7949602519874007No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAAGC2300.6899655017249138No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC2230.6689665516724164No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT2040.6119694015299235No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA2040.6119694015299235No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA1950.5849707514624268No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG1930.5789710514474277No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG1800.5399730013499325No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG1800.5399730013499325No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA1670.5009749512524374No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA1640.4919754012299385No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT1520.455977201139943No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG1460.4379781010949452No Hit
GCGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTG1450.43497825108744564No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC1440.43197840107994606No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT1440.43197840107994606No Hit
TCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACGTGCA1360.40797960101994896No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT1240.3719814009299535No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT1230.3689815509224539No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG1190.3569821508924554No Hit
CATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGAC930.2789860506974651No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC790.2369881505924704No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT760.2279886005699715No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG720.21598920053997303No Hit
TCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGA720.21598920053997303No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG700.20998950052497373No Hit
ACATCTCTGCCAAACCCCAAACCTACGCACCGACCTACTGTAGATGACGT660.19799010049497526No Hit
GGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTG630.18899055047247637No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN620.18599070046497676No Hit
TTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCA600.17999100044997748No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT580.17399130043497826No Hit
TCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGAC580.17399130043497826No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG560.16799160041997901No Hit
TGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGACG520.1559922003899805No Hit
ATTAAGTTATTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTAAGAGTGAG460.13799310034498274No Hit
ATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACG450.13499325033748313No Hit
GTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGCA430.12899355032248389No Hit
GGGTTTTGCAGTCCTTAGCTGTTACAGAAATTAAGTATTGCAACTTACTG410.12299385030748462No Hit
CGTAACCTCAACACCACCTTCTTCGACCCAGCCGGAGGAGGAGACCCCAT400.119994000299985No Hit
CGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGC400.119994000299985No Hit
TTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAAGCT390.11699415029248537No Hit
ATTAAGTTACTAGCTCAGGAGTTTGATAGCTCTTGGGCAGTAAGGGTGAG390.11699415029248537No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT370.11099445027748613No Hit
TCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAA360.10799460026998652No Hit
CCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACGTGC350.10499475026248686No Hit
TGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACGT340.10199490025498724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)