FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003032200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003032200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519769
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG239564.608970523444069No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT235044.522008815454558No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA217544.185320786734107No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA193163.716266264436702No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA190943.6735549830790215No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT162203.120617043340407No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA141982.7315980752988347No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT135842.613468675507774No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA134702.591535855351127No Hit
CAATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCAC132402.5472854287192965No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA117492.260427228249472No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA112872.1715415886672735No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT108092.079577658536773No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT107582.069765607414063No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT100561.934705609607345No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG99631.9168130457953438No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT98501.895072618797966No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA98191.8891084308606323No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT96601.8585179185368883No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA92981.7888715948815723No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC89941.7303840744638483No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA89221.7165317669964926No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA83811.6124470678320562No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA83811.6124470678320562No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC83271.6020578372315395No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA82901.5949392903385928No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT77031.48200450584779No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA73011.4046624558217207No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC71981.3848459604170316No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG68811.3238573289288127No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT66221.2740275006781858No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT63291.217656305012419No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT56611.0891376746208412No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC55861.074708187675679No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG54981.057777589660022No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT54261.0439252821926663No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC52731.0144891288245355No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC51300.9869769070490929No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT49080.9442656256914129No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA45750.8801987036548928No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT45260.8707714388507202No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG41390.7963152862136833No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA40940.787657594046586No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG38710.7447539195296372No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG38630.7432147742554865No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT34110.6562530662659758No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC22580.434423753629016No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGGTTTGGTGGAAAT18960.3647774299736999No Hit
GATGATTAGTTCTTCTATGATAGGAGAAGTAGCGTCTTGTAGACCTACTT13600.2616546966056075No Hit
TTTTAGCAAAATAGTAACTATTTTTGCTTAAATGAGATTGCACATGGATT13340.256652474464618No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTGGTATTGGTTATGGTTC10620.2043215351434964No Hit
AATATTTTTTTATTATAGGAAATGGCTGAGTGTGGTGGCTCTGTAATCCC9600.1846974328980759No Hit
GGCGATTAGCTCTTCTATGATAGGGGATGTGGCGTCTTGAAGACCTAGCT9460.18200392866831228No Hit
AGAGAACCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG9310.17911803127927983No Hit
AATAAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC8080.15545367268921384No Hit
TCAGCCCCATTTTATTTTTTTAATTTATTTTTGAGACAGTGTCTCACTCT7550.14525683524796593No Hit
GCCGGCCTCCACTATAGCAGATGCAAGCAGGAGTAGGAGAGAGGGGGGTA7110.13679153624013746No Hit
GGCCTTCTCCACTTCAAGCCAACTAGGACTTATAGTAGTCACAATCGGCA7010.13486760464744915No Hit
TATCATAGAAGAGCTTATCATCTTTCATGATCACGCCCTCATAATCATTT6650.12794145091377132No Hit
TAACCAATACCACCAATCAATACTCATCATTAATAATCATAATGGCTATA6270.12063051086155581No Hit
AACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT6150.11832179295032987No Hit
TTCTCTTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTACAGG5730.11024128026103903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)