FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003032533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003032533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21900
Sequences flagged as poor quality0
Sequence length223-233
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA251611.488584474885846No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA17988.210045662100457No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT16277.429223744292238No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT10434.76255707762557No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA10144.63013698630137No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT9604.383561643835616No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA9124.164383561643835No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT7803.5616438356164384No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA7453.4018264840182644No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC7003.1963470319634704No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC6743.0776255707762554No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA6593.0091324200913245No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA5842.666666666666667No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT5662.584474885844749No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA5322.4292237442922375No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT5112.3333333333333335No Hit
CAATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCAC4522.0639269406392695No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC4462.036529680365297No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG3831.7488584474885844No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG3551.6210045662100456No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT3281.4977168949771689No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA3051.3926940639269407No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT2681.223744292237443No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG2611.1917808219178083No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT2411.100456621004566No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC2371.0821917808219177No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA2231.0182648401826484No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA2140.9771689497716896No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT2090.9543378995433789No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG2030.9269406392694064No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT1950.8904109589041096No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGTTTGGTGGAAATTT1480.6757990867579908No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG1150.5251141552511416No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC1090.497716894977169No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT1060.4840182648401826No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA1010.4611872146118722No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA880.4018264840182648No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT610.2785388127853881No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA570.2602739726027397No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA520.2374429223744292No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG410.1872146118721461No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT310.1415525114155251No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT290.1324200913242009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)