FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003032616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003032616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length211-220
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC65.714285714285714No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT43.8095238095238098No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT43.8095238095238098No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG43.8095238095238098No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT32.857142857142857No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG32.857142857142857No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG32.857142857142857No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT32.857142857142857No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG32.857142857142857No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC32.857142857142857No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG32.857142857142857No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC32.857142857142857No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG32.857142857142857No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA21.9047619047619049No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG21.9047619047619049No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT21.9047619047619049No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC21.9047619047619049No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT21.9047619047619049No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG21.9047619047619049No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT21.9047619047619049No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA21.9047619047619049No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT21.9047619047619049No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT10.9523809523809524No Hit
TCGCCGATACGGCTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC10.9523809523809524No Hit
CCTCCTCAAGTATACTTCAAAGGACATATAACTACAACCCCCACGCACTT10.9523809523809524No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA10.9523809523809524No Hit
GCCTAGCCATCTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGTC10.9523809523809524No Hit
ACTCCCTCTTAGACAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC10.9523809523809524No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC10.9523809523809524No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC10.9523809523809524No Hit
TATAAACTTCAGGGTGAACGAAAAATCAGAATAGGTGTGGGTATAGAATG10.9523809523809524No Hit
TACGAGCTTGTTTGCGCTACTGCTCGTAGTGCGCTGATAAGGGCTGCGGT10.9523809523809524No Hit
GAGGGTGAACTCACTGGAATGGGGATGCTTGCATGTGTAATCTTACTAAG10.9523809523809524No Hit
CGAGCAGTAACCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT10.9523809523809524No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG10.9523809523809524No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC10.9523809523809524No Hit
CACCCTCTAATTTACCACGATCTAAAGGGACTGGCATCAAGCGCGCAGCA10.9523809523809524No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGATTTTAT10.9523809523809524No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT10.9523809523809524No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA10.9523809523809524No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT10.9523809523809524No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGACTCCATAAAGTGATTGGC10.9523809523809524No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG10.9523809523809524No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC10.9523809523809524No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTGCATCCTCGCCTT10.9523809523809524No Hit
GGTTGACCGGTTAGGGGGAGAAGAAGTATTGGAGTGCTATAGGCGCTTGT10.9523809523809524No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT10.9523809523809524No Hit
AACTCCTGAGCCAATAACTTAATATGGCTAGCGTACACAATAGCTTTTAT10.9523809523809524No Hit
TATAAACTTCAGGGTGACAGAAAAATCAGAATAGGTGTTGGTATAGAATG10.9523809523809524No Hit
GAGGGTGGACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG10.9523809523809524No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCACAACCCTCCTCATACTAGGC10.9523809523809524No Hit
CCTCCTCACGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT10.9523809523809524No Hit
AATCCTAGGCCTACCCGCCGCAGGACTGATCATTCTATTTCCCCCTCTAT10.9523809523809524No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCTGGAGGATAATG10.9523809523809524No Hit
GGGCTATTTAGACTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA10.9523809523809524No Hit
ACTCCCTCTTAGCAAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC10.9523809523809524No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG10.9523809523809524No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT10.9523809523809524No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA10.9523809523809524No Hit
TCGCCGATACGCTTGAATAGGATTGCTTGAATGGCTTCCGTGTTGGCATC10.9523809523809524No Hit
AGAGTCCATATCAAAAATAGGGTTTACGACCTCGATGTTGGATCAGGACA10.9523809523809524No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA10.9523809523809524No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGCGTGCTATAGGCGCTTGT10.9523809523809524No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGACCGGAGGCCATTAGGAGGG10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)