FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003032650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003032650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length211-220
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT66.666666666666667No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA33.3333333333333335No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT33.3333333333333335No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA33.3333333333333335No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG33.3333333333333335No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT33.3333333333333335No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC33.3333333333333335No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG33.3333333333333335No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG33.3333333333333335No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC22.2222222222222223No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT22.2222222222222223No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT22.2222222222222223No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG22.2222222222222223No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC22.2222222222222223No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG22.2222222222222223No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC22.2222222222222223No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG22.2222222222222223No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT22.2222222222222223No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG22.2222222222222223No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATTATTCTATTTCCCCCTCTAT11.1111111111111112No Hit
GGTTGACCAGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT11.1111111111111112No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC11.1111111111111112No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC11.1111111111111112No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA11.1111111111111112No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG11.1111111111111112No Hit
CCCTACGAGTACACGGACTACGCCGGACTCTTCTTTAACTCATACATACT11.1111111111111112No Hit
CCTCCTCAAATATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT11.1111111111111112No Hit
GTGTGAGGAGGTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG11.1111111111111112No Hit
GGTTGACCTGTTAGGGTGAGACGAATTATTCGAGTGCTATAGGCGCTTGT11.1111111111111112No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC11.1111111111111112No Hit
CAAGCAATCCTATACAACCGTATCGGCGACATCGGTTTCATCCTTGCCCT11.1111111111111112No Hit
AATCCTAGGCCTACCCGCCGCAGTCCTGATCGTTCTATTTCCCCCTCTAT11.1111111111111112No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT11.1111111111111112No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA11.1111111111111112No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTCTTTAGGTAGTGGGTGTTG11.1111111111111112No Hit
CCTCTCCCCCAACCCCGACATCATTACCGCGTTTTCCTCCTGTATATATA11.1111111111111112No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT11.1111111111111112No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT11.1111111111111112No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA11.1111111111111112No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT11.1111111111111112No Hit
TGAATCGGAGGACAACCAGTAAACTACCCTTATACCATCATTGGACAAGT11.1111111111111112No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGCGACCCCAT11.1111111111111112No Hit
AATGCTTCACTCAGCCATTTTCCCTCACCCCCACTGATGTTCGCCGACCG11.1111111111111112No Hit
AACTCCTGAGCCAACAACTTAATATCACTAGCTTACACAATAGCTTTTAT11.1111111111111112No Hit
CCTCCTCAAGTATACTTCAAAGCACATTTAACTAAAACCCCTACGCATTT11.1111111111111112No Hit
CATGAGTTTGTTTGAGCTACTGCACCCAGTGCGCCGACCCGTGTGTAGAT11.1111111111111112No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG11.1111111111111112No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT11.1111111111111112No Hit
CACCCCCTAAATCACCACGATAACAATGGACAAGCATCAAGCACGCAACA11.1111111111111112No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG11.1111111111111112No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT11.1111111111111112No Hit
GGGCTATTTAGGCTTTATGGCCCTGCAGTAGGAACCAGATGTCGGCTACA11.1111111111111112No Hit
TATAAACTTCAGGCTGACCGAAAAATCAGAATAGGTTTAGGTATAGAATG11.1111111111111112No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC11.1111111111111112No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA11.1111111111111112No Hit
AGTTGAGTAGTAGGAATGCGGTAGGAGTTAGGATAATAGAAGTAGTTAAA11.1111111111111112No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT11.1111111111111112No Hit
TGAATCGGAGGACAACCAGTAAGCAACCCTTTTACCATCATTGGACAAGT11.1111111111111112No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTTCCAG11.1111111111111112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)