FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003032970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003032970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282582
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT173626.144057300181894No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA158415.605806456179092No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG137714.873275721737407No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA135064.779497632545597No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC109623.8792279763042234No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA108483.838885703972652No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA106023.751831326836104No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT102723.635051064823662No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA98373.4811134467163516No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA81202.873502204669795No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA79512.81369655533615No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA79342.8076806024446No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT78972.7945870579159324No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG76852.7195645865624845No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT69962.4757415546637787No Hit
TATCATAGAAGAGCTCATCACCTTTCATGATCACGCCCTCATAATCATTT69622.4637096488806787No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC68012.4067350362018813No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT61832.1880374546149435No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC60242.1317706010998574No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT54591.9318286373512823No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT52171.8461897785421577No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT51721.8302651973586426No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC49041.7354254694212652No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC48881.729763396111571No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA48571.7187931290740386No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT48411.7131310557643447No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT45751.6189990869906787No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA42951.5199128040710308No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA36261.283167363809443No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG32641.1550629551776121No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA32221.140200012739665No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA32041.133830180266259No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT29311.037221054419602No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG28741.0170499182538166No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG26190.9268106248805656No Hit
GGTGATGAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT24930.882221797566724No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT23050.8156924361778175No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC21770.770395849700264No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG20820.7367772894239548No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA20260.7169600328400252No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC16080.5690383676242649No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT13150.4653516501404902No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA10780.3814821892406452No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT9730.34432483314577716No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGGTTTGGTGGAAAT8110.2869963408851236No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG7370.260809251827788No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA6020.21303550827724343No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC3770.13341260235966906No Hit
CACTTACACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA3560.12598113114069545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)