FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003033004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG106.578947368421052No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT95.921052631578947No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA85.263157894736842No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA74.605263157894736No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG63.9473684210526314No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA63.9473684210526314No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA63.9473684210526314No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA53.289473684210526No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA42.631578947368421No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC42.631578947368421No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT42.631578947368421No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG42.631578947368421No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT31.9736842105263157No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT31.9736842105263157No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA31.9736842105263157No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG31.9736842105263157No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT31.9736842105263157No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT31.9736842105263157No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA31.9736842105263157No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA31.9736842105263157No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT31.9736842105263157No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA31.9736842105263157No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA21.3157894736842104No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT21.3157894736842104No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG21.3157894736842104No Hit
CAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA21.3157894736842104No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC21.3157894736842104No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT21.3157894736842104No Hit
GTAAAATGGCTGCTGTGTTGGCATAGAACTGATTAGCCGCATTGCCACTC10.6578947368421052No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT10.6578947368421052No Hit
CAATGACCCCAATACACAAAACTAACCCCCTAATAAAATTAATTAACCAC10.6578947368421052No Hit
ACTGATGATAAAGGAGGAGGCGACAATGGATTTTACATAATGTGGGTATG10.6578947368421052No Hit
GATAAGGGGTGTAGGTGCGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT10.6578947368421052No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT10.6578947368421052No Hit
TACCTCCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT10.6578947368421052No Hit
TCAGCCCCATTTTATTTTTTTAATTTATTTTTGATACAGTGTCTCACTCT10.6578947368421052No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA10.6578947368421052No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC10.6578947368421052No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT10.6578947368421052No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT10.6578947368421052No Hit
CGCCCACGGGCTTACATCTTCATTACTATTCTGCCTAGCATACTCAAACT10.6578947368421052No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGTTTGGTGGAAATTT10.6578947368421052No Hit
GACGCACTCTCCCCTGAACTCTAAACAACATATTTTGTCACCAAGACCCT10.6578947368421052No Hit
TCCGGCCTCCACTATAGCGGATGCGAGGAGGAGTTGGAGAGAGGGAGGTA10.6578947368421052No Hit
TCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT10.6578947368421052No Hit
CGCCCACGGACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT10.6578947368421052No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT10.6578947368421052No Hit
CAGTGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTGT10.6578947368421052No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC10.6578947368421052No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG10.6578947368421052No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA10.6578947368421052No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC10.6578947368421052No Hit
TACTTGACCACCTGTAGTACATAAAAACCAAATCCACATCAACACCCCCC10.6578947368421052No Hit
CAGTGTGGTACGGGCGATGCGTGTGGGGAGGCACGGAGTGGGTTTTGTGT10.6578947368421052No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA10.6578947368421052No Hit
ATGTAGGGTGTTGAGGGTTATGAGAGTAACAAAGATATGTCCGCATCGCC10.6578947368421052No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA10.6578947368421052No Hit
TCCCCCAGGCTCCTGGCAGTTCTAGGCAATATCAAGCCTCCTAGAGAGGA10.6578947368421052No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG10.6578947368421052No Hit
CCCCCCCTACCCTCTCTAGAGCCCACTGTAAAGCTAACTTACCATTAACC10.6578947368421052No Hit
ACTCCTTCCTATCTCCCCTTATAAACAAATAATCTTATCGACATTTAGGT10.6578947368421052No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACACGCTGAACAGTCTA10.6578947368421052No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCATGAGTAGGAGAGAGGGAGGTA10.6578947368421052No Hit
CTAGCCCCTACCCCCAAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC10.6578947368421052No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC10.6578947368421052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)