FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003033005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152
Sequences flagged as poor quality0
Sequence length211-220
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG85.263157894736842No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG85.263157894736842No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT74.605263157894736No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG63.9473684210526314No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC53.289473684210526No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT53.289473684210526No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT42.631578947368421No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG42.631578947368421No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT42.631578947368421No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG42.631578947368421No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT42.631578947368421No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC31.9736842105263157No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA31.9736842105263157No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT31.9736842105263157No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA31.9736842105263157No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT31.9736842105263157No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT31.9736842105263157No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA31.9736842105263157No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC31.9736842105263157No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG31.9736842105263157No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA21.3157894736842104No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT21.3157894736842104No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG21.3157894736842104No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA21.3157894736842104No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG21.3157894736842104No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA21.3157894736842104No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA10.6578947368421052No Hit
TACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGAT10.6578947368421052No Hit
TAAACTTAAACTCCAGCACCGCAACCCTACTACTAACTCGCACCTGAAAC10.6578947368421052No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGGAACGACCTACTGTAG10.6578947368421052No Hit
TTGAGGGATAGGAGGGGGATGGGGGATAGGATAATGAACATGAGGGTGTT10.6578947368421052No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC10.6578947368421052No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGTTTTCATCCTCGCCTT10.6578947368421052No Hit
ACTGCTTAACTCAGCCATTTTACCACACCCCCACTGATGTTCGCCGACCG10.6578947368421052No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG10.6578947368421052No Hit
AAGTATACGTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT10.6578947368421052No Hit
AATGCTTCACTCAGCCATTTTACCTTACGCCCACTGATGTTCGCCGACCG10.6578947368421052No Hit
CTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAG10.6578947368421052No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTGGCCGACAG10.6578947368421052No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACGCTAGCCATCATTCT10.6578947368421052No Hit
TTGAGGGATAGGAGGAGCATGGGGGCTAGGTGTATGAACATGAGGGTGTT10.6578947368421052No Hit
GCGGCTTCGAAGCCAAAGAGATGTTTGGATGTAAAGTGAAATATTAGTTG10.6578947368421052No Hit
TCGCCGATAGGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC10.6578947368421052No Hit
GGACATAACCTATGACGGCTGTTCCTATCGTTGCAAGCAGGAGGACAATG10.6578947368421052No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGCTCTCCTCTTGTAAATATA10.6578947368421052No Hit
CCTCCTCAAGTATACTTCAAAGGACACTTAACTAAAACCCCTACGCATTT10.6578947368421052No Hit
TATAAACTTCAGGGTGACCGAAAGACCAGAATAGGAGATGGTATAGAATG10.6578947368421052No Hit
CCTATCCCTCAACCACGACATCGTTAACGACATTCCCTCTTGTAAATATA10.6578947368421052No Hit
TCTCCGTGCCACCTATCACACCCCATCCTATAGTAAGGTCAGCTAAATAA10.6578947368421052No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGTCGGAGGTCATTAGGAGGG10.6578947368421052No Hit
ATTAAGTTGTTGATTCCGGTGTTTGATAGTTCTTGGGCAGTGAGAGTGAG10.6578947368421052No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC10.6578947368421052No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCAGAGGTCATTAGGAGGG10.6578947368421052No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT10.6578947368421052No Hit
ATGTTGATGAGATATTTCGAGGTGGGGATCAATAGAGGGGGACATAGAAT10.6578947368421052No Hit
TCGCCGTTACGGTTGTATAGGGTTGCTTGAATGGCTGCTGTGTTGGCATC10.6578947368421052No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG10.6578947368421052No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT10.6578947368421052No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT10.6578947368421052No Hit
CCCCCCCCAGTATAATTTTAAAGAAACATAACAAAAAACCCCACCCCCCT10.6578947368421052No Hit
CATTTTAGGGGTATGGGACCGATAGCATATTAAACTGACCTTACTTTAGG10.6578947368421052No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG10.6578947368421052No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT10.6578947368421052No Hit
TCAACTTAAACTCCAGCACCACAACCCTACTACTATCTCGCACCTGAAAC10.6578947368421052No Hit
AGTTGAGTAGTAGGAAGGCGGTAGTAGTTAGGATAATATAAATAGTTAAA10.6578947368421052No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTATTTTAGG10.6578947368421052No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC10.6578947368421052No Hit
GGTTGACCTGTTAGGGTGAAAAGAATTATTCGAGTGCTATAGGCGCTTGT10.6578947368421052No Hit
CGCCACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT10.6578947368421052No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT10.6578947368421052No Hit
AAGCATTGAACTGTAGATCTAAAGTAAGGAGCTACGTTTCTTTTTACAAG10.6578947368421052No Hit
AATCCTACCTCCATCGCTAAACCCACTAAAACACTCACCAAGACCTCAAC10.6578947368421052No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG10.6578947368421052No Hit
GAGGGTGAACTCACTGGAACGGGGATACTTGCATTTGTAATCTTACTAAG10.6578947368421052No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCTACCCTTTCTAAC10.6578947368421052No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC10.6578947368421052No Hit
GGCTGACCAGCTAGGGTGAGTAGAATTAGTCGAGTGCTATAGGCGCTTGT10.6578947368421052No Hit
CCTCCTCAAGTATACTTGAAAGGACATTTAACTAAAACCCCTACGCATTT10.6578947368421052No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCTAC10.6578947368421052No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG10.6578947368421052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)