FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003033027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142087
Sequences flagged as poor quality0
Sequence length210-223
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT85376.008290695137486No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC58994.151681716131666No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC54863.8610147304116493No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC50913.583016039468776No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG48723.4288851196801957No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC37282.6237446071772927No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT37072.608964929937292No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT36892.5962966351601486No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC34502.428089832285853No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG34192.406272213502995No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA33552.3612293876287063No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG30152.12193937517155No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG29782.0958989914629766No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT29482.074785166834404No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG27171.9122087171943951No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG26011.8305685952972475No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG25491.7939712992743884No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG25361.7848219752686734No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT25191.7728574746458157No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG25141.7693385038743867No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC23741.6708073222743813No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT22761.6018354951543774No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG22451.580017876371519No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC21861.5384940212686593No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT21511.513861225868658No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG20761.4610766642972264No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC19951.4040693378000801No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA19411.3660644534686495No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT18171.278793978337216No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT17671.2436042706229282No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT17011.1971538564400686No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA15971.12395926439435No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT15741.1077719988457775No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT15561.095103704068634No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA15061.0599139963543462No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG14261.003610464011486No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA13310.9367500193543392No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT13260.9332310485829104No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG13100.9219703421143384No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG12730.8959299584057655No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA12420.874112339622907No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA11540.8121784540457607No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA11150.7847304820286163No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG9270.6524171810228945No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA9190.6467868277886084No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT8160.5742960298971758No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC7600.5348835572571734No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT7500.5278456157143159No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC6040.4250916691885957No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG5990.421572698417167No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG4540.31952254604573255No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG4010.2822214558685876No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCATGT3780.2660341903200152No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG3260.22943689429715594No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT2820.19846995150858276No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG2780.19565477489143973No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG2390.16820680287429532No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC2370.1667992145657238No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA1970.13864744839429366No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT1970.13864744839429366No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC1550.10908809391429194No Hit
ACGACCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA1550.10908809391429194No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT1540.1083842997600062No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT1440.10134635821714864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)