FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003033069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length211-221
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT77.368421052631578No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC66.315789473684211No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT66.315789473684211No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT55.263157894736842No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT55.263157894736842No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT44.2105263157894735No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG44.2105263157894735No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA33.1578947368421053No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC33.1578947368421053No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA33.1578947368421053No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT33.1578947368421053No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA22.1052631578947367No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA22.1052631578947367No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT22.1052631578947367No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG22.1052631578947367No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG22.1052631578947367No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC22.1052631578947367No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG22.1052631578947367No Hit
TCTCCGTCCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA11.0526315789473684No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC11.0526315789473684No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT11.0526315789473684No Hit
TCGCGGATACGGTAGTATAGGGTAGCTTGAATGGCTGCTGTGTTGGCATC11.0526315789473684No Hit
TAATTCTTCTCACCCTAACAGGTAACCCTCGCTTCCCCACCCTAACTAAC11.0526315789473684No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCTCTATT11.0526315789473684No Hit
TCGCCGATACGGTTGTATAGGATTGCTTTAATGGCTGCTGTGTTGGCATC11.0526315789473684No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG11.0526315789473684No Hit
CGCAACCTCAACACCACCTTCACCGACCCCGCCGGAGGAGTAGACCCCAT11.0526315789473684No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGAGCTTGT11.0526315789473684No Hit
CCTCCTCAAATATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT11.0526315789473684No Hit
TCTCCGTGCCCCCTATCTCAACCCATCCTAAAGTAACGTCAGCTAAATAA11.0526315789473684No Hit
GCCTAGCCATGTGCTTTCACTTCCACTCCATAACGCTCCTCATACTAGGC11.0526315789473684No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT11.0526315789473684No Hit
CGAGCAGTAGCCCAAACAATCTCATATGGAGTCACCCTAGCCATCATTCT11.0526315789473684No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC11.0526315789473684No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG11.0526315789473684No Hit
GGACATAGCCTATGAAGGATGTTGCTATAGTTGCAAGCAGGAGGTTAATG11.0526315789473684No Hit
CGAGCAGTAGCCCAAACAATCTCATACGAAGTTACCCTAGCCATCATTCT11.0526315789473684No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC11.0526315789473684No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA11.0526315789473684No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT11.0526315789473684No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG11.0526315789473684No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT11.0526315789473684No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT11.0526315789473684No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA11.0526315789473684No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT11.0526315789473684No Hit
ACCTACGAGTACACCGACTACTGCGGACTAATCTTCAACTCCTACATACT11.0526315789473684No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT11.0526315789473684No Hit
GTGGGAGGAGTTCAGTTATATGTTTGGGATTCTTTAGGTAGTGGGTGTTG11.0526315789473684No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG11.0526315789473684No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)