FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003033442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71436
Sequences flagged as poor quality0
Sequence length209-222
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG1136215.90514586483006No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA27663.871997312279523No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC27143.7992048826922002No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT17872.501539839856655No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT14312.0031916680665214No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC14181.9849935606696905No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT13971.955596617951733No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC13961.9541967635365924No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC13901.9457976370457473No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTACTTCCCCCTCTAT11961.674225880508427No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC11501.609832577411949No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG10691.4964443697855423No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT10611.4852455344644158No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT10141.419452376952797No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA10061.4082535416316704No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG9761.3662579091774456No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT9361.3102637325718125No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG9321.3046643149112491No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG9201.2878660619295594No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG9081.2710678089478695No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC9051.266868245702447No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG8981.2570692647964612No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT8441.1814771263788566No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC7811.0932862982249845No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG7601.0638893555070272No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG7020.9826977994288595No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA6640.9295033316535081No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG6630.9281034772383672No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG6260.8763088638781567No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC6200.8679097373873117No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCATAT5800.8119155607816788No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA5370.7517218209306232No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG5280.7391231311943558No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG5170.7237247326278067No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA5140.7195251693823843No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA5080.7111260428915394No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC4940.6915280810795678No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG4870.6817291001735819No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT4660.6523321574556246No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT4650.6509323030404838No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA4550.6369337588890756No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAGTAGAAT4520.6327341956436531No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT4360.6103365250013999No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG4320.6047371073408365No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT4320.6047371073408365No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA4230.5921384176045691No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG4010.561341620471471No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG3850.5389439498292178No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT3810.5333445321686545No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA3790.5305448233383728No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC3540.4955484629598521No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC3400.4759505011478806No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG3380.473150792317599No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG3330.4661515202418949No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT3310.4633518114116132No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA3220.45075312167534576No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG2980.417156615711966No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC2640.3695615655971779No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT2550.35696287586091047No Hit
ACGACCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA2190.30656811691584074No Hit
TTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCA1980.27717117419788345No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG1460.20437874461056052No Hit
TCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAT1460.20437874461056052No Hit
GGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTG1440.20157903578027886No Hit
GCGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTG1320.18478078279858895No Hit
TCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACGTGCA1300.1819810739683073No Hit
TTACCTCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA1280.17918136513802566No Hit
TCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACG1220.1707822386471807No Hit
TCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGA1150.16098325774119493No Hit
CATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGAC1140.15958340332605409No Hit
TCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGAC1030.144185004759505No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1020.14278515034436418No Hit
TTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCATATC960.13438602385351925No Hit
TTACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGACG930.13018646060809674No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT850.11898762528697016No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT800.11198835321126603No Hit
CCCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGAT760.10638893555070272No Hit
TTACCTCACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACT760.10638893555070272No Hit
ACTCCTCCTAGACCTAACCTGACACCTACGCACCGACCTACTGTAGATGA760.10638893555070272No Hit
TCCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGAT740.10358922672042109No Hit
ACATCTCTGCCAAACCCCAAACCTACGCACCGACCTACTGTAGATGACGT730.10218937230528026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)