FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003033500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148988
Sequences flagged as poor quality0
Sequence length210-223
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG89315.9944425054366794No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT62714.209063817220179No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG59513.9942814186377427No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT47693.200928933873869No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC46843.1438773592504097No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT38652.594168657878487No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT38242.5666496630601126No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG37912.5445002282062985No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT36812.4706687786935864No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG35802.4028780841410047No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT33702.261927135071281No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC33232.2303809702794855No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA32742.19749241549655No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG31732.1297017209439684No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA31032.0827180712540607No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC30302.033720836577442No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT29551.9833812119096839No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG29531.9820388219185439No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT29131.9551910220957394No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG27021.8135688780304453No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC27021.8135688780304453No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG26871.8035009530968937No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC26271.763229253362687No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC24721.6591940290493195No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA23551.5806642145676162No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA23261.561199559696083No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG23231.5591859747093726No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG23191.5565011947270921No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC23051.5471044647891106No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG22201.490052890165651No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC22131.4853545251966602No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT21491.4423980454801728No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT20591.3819904958788627No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT20491.3752785459231616No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA20141.3517867210782077No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA18921.2699009316186538No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT18831.263860176658523No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT17661.1853303621768196No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT17001.1410314924691922No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG16531.1094853276773968No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG15851.0638440679786292No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA15541.0430370231159556No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG14660.9839718635057858No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC13680.9181947539399147No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA12950.8691975192632965No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT11860.7960372647461541No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG8090.5429967514162215No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT7980.5356136064649502No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT6360.4268800171825919No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT6000.4027169973420678No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT4190.2812307031438773No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT3000.2013584986710339No Hit
TATTTTTGGGCTGTTGCTTTTTACTTGTCATTTTTGTGTTGTAAACCCTT2210.14833409402099498No Hit
CGTAACCTCAACACCACCTTCTTCGACCCAGCCGGAGGAGGAGACCCCAT1990.13356780411845248No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAAGCCTCTTTTTACCAG1850.12417107418047091No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCACAACCCTCCTCATACTAGGC1620.10873358928235831No Hit
GGCTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT1550.10403522431336751No Hit
GTGCAAGGAGTTCAGTTATGTGTTTGGGATTTTTTAGATGACAGGTGTCG1530.1026928343222273No Hit
TCAACTTAAACTCCAGCACCACAACCCTACTACTATCTCGCACCTGAAAC1490.10000805433994685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)