FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003033511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214622
Sequences flagged as poor quality0
Sequence length223-233
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT119695.576781504226035No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT91234.25072918899274No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG87454.074605585634278No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA84923.9567239146033497No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA70953.3058120789108294No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA69203.2242733736522817No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT62952.933063712014612No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT62442.909301003624978No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT58232.7131421755458436No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA56952.6535024368424485No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA51192.3851236126771718No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA50152.336666324980664No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT48672.2677078771048635No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG47992.236024265918685No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA47782.226239621287659No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC47582.216920912115254No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA47262.202010977439405No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA46962.188032913680797No Hit
CAATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCAC43912.0459225988016136No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT41621.939223378777572No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA40211.8735264791121136No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT40081.8674693181500497No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC39761.8525593834742011No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC39381.8348538360466307No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG39061.819943901370782No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT37341.7398030024880953No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT35661.6615258454398898No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT35431.6508093298916233No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT35421.6503433944330033No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT35111.6358993952157748No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG34181.5925673975640895No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG34071.5874421075192664No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA32791.5278023688158715No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC30941.4416043089711215No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA30691.4299559225056147No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA27281.2710719311161018No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT27011.2584916737333545No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT26891.252900448229911No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC24661.1489968409575906No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA23501.094948327757639No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT22181.033444847219763No Hit
CCCCCTCTACCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTA18970.8838795650026559No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA18340.8545256311095787No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGGTTTGGTGGAAAT14500.6756064149993942No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG14370.6695492540373308No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC9960.4640717167857908No Hit
AGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGAT5820.2711744369169983No Hit
GGCGATTAGCTCTTCTATGATAGGGGATGTGGCGTCTTGAAGACCTAGCT5810.270708501458378No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA5480.255332631323909No Hit
GATGATTAGTTCTTCTATGATAGGAGAAGTAGCGTCTTGTAGACCTACTT5310.24741172852736437No Hit
TTTTAGCAAAATAGTAACTATTTTTGCTTAAATGAGATTGCACATGGATT4950.23063805201703458No Hit
TCAGCCCCATTTTATTTTTTTAATTTATTTTTGAGACAGTGTCTCACTCT3370.15702024955503163No Hit
AGAGAACCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG2920.1360531539171194No Hit
AATATTTTTTTATTATAGGAAATGGCTGAGTGTGGTGGCTCTGTAATCCC2820.13139379933091666No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGGGTTTGGTGGAAA2520.11741573557230853No Hit
TTCTCTTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTACAGG2240.1043695427309409No Hit
TTAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA2160.10064205906197873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)