FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003033542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22045
Sequences flagged as poor quality0
Sequence length210-222
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC21159.59401224767521No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT10944.962576547970062No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG9424.273077795418462No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC9194.168745747334997No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC8543.8738943070991154No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG8473.842141075073713No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC7033.1889317305511455No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC6803.0845996824676796No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA6482.9394420503515537No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT5002.2680880018144705No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG4602.0866409616693127No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT4241.923338625538671No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG4091.8552959854842368No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT3431.5559083692447269No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT3361.524155137219324No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG2851.292810161034248No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG2851.292810161034248No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG2821.2792016330233613No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC2771.2565207530052165No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC2641.1975504649580404No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT2611.1839419369471536No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG2611.1839419369471536No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAAGC2471.1204354728963484No Hit
GCGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTG2341.0614651848491723No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC2241.0161034248128828No Hit
TCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACGTGCA2221.0070310728056249No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT2221.0070310728056249No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA2090.9480607847584486No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT1870.848264912678612No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG1780.8074393286459515No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT1770.8029031526423225No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT1770.8029031526423225No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA1760.7983669766386935No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT1720.7802222726241778No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC1600.7257881605806306No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG1560.7076434565661147No Hit
CATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGAC1490.6758902245407121No Hit
TCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGA1380.6259922885007939No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG1310.5942390564753912No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG1280.5806305284645044No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA1140.5171240644136993No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG1100.4989793603991835No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA1040.47176230437740985No Hit
GGGTTTTGCAGTCCTTAGCTGTTACAGAAATTAAGTATTGCAACTTACTG970.44000907235200726No Hit
TGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGACG950.4309367203447494No Hit
GGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTG940.4264005443411204No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC930.4218643683374915No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG890.40371966432297574No Hit
TGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGCAA890.40371966432297574No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG850.38557496030845995No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG820.37196643229757315No Hit
ACATCTCTGCCAAACCCCAAACCTACGCACCGACCTACTGTAGATGACGT760.3447493762757995No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG760.3447493762757995No Hit
TTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCA750.34021320027217056No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT750.34021320027217056No Hit
GTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGCA730.3311408482649127No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN710.3220684962576548No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA670.30392379224313903No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT660.2993876162395101No Hit
TCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGAC630.28577908822862325No Hit
TCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAA610.2767067362213654No Hit
AATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGA610.2767067362213654No Hit
ATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACG590.2676343842141075No Hit
TGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACGT590.2676343842141075No Hit
TTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAAGCT570.25856203220684965No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA560.2540258562032207No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA490.22227262417781812No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG480.21773644817418913No Hit
CGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGC480.21773644817418913No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA470.2132002721705602No Hit
CCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACGTGC450.20412792016330233No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT450.20412792016330233No Hit
CAATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATG440.19959174415967337No Hit
GAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGACGT410.18598321614878657No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT390.17691086414152868No Hit
GTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGCAAGC380.17237468813789975No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG330.14969380811975505No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT320.1451576321161261No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC310.14062145611249716No Hit
GTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGC270.12247675209798141No Hit
GCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACGTG260.11794057609435246No Hit
CTCCTCCTAGACCTAACCTGACACCTACGCACCGACCTACTGTAGATGAC240.10886822408709457No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA240.10886822408709457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)