FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003033953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003033953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46813
Sequences flagged as poor quality0
Sequence length209-223
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG16593.543887381710209No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT16413.505436524042467No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC15003.204238138978489No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT14373.0696601371413923No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT13972.9842137867686325No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC12512.6723346079080597No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG11832.527075812274368No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC11412.4373571443829705No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT10992.3476384764915728No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT10462.234422062247666No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG10312.2023796808578813No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG10102.1575203469121824No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT9902.1147971717258027No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC9542.037895456390319No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT9442.016533868797129No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC9432.0143977100378097No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG9021.9268152009057313No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG8721.8627304381261616No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT8191.7495140238822549No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG8111.7324247538077029No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA8071.7238801187704271No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG7941.6961100548992802No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC7941.6961100548992802No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA7801.666203832268814No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG7731.6512507209535812No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG7681.6405699271569862No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG7511.6042552282485634No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC6991.4931749727639758No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA6851.4632687501335098No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCACTA6071.2966483669066284No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG5991.2795590968320767No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT5541.183431952662722No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA5261.12361950740179No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA4991.0659432209001773No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG4520.9655437592121846No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC4510.9634076004528657No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT4490.9591352829342277No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA4410.9420460128596757No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA4170.8907782026360198No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA4160.888642043876701No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT4130.882233567598744No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC3910.835238074893726No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG3620.7732894708734753No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA3470.7412470894836904No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT3460.7391109307243715No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG3420.7305662956870955No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT3340.7134770256125436No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT2910.6216221989618268No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC2820.6023967701279559No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC2720.581035182534766No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG2660.5682182299788521No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT2640.5639459124602141No Hit
TTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCA2030.43364022814175546No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG1710.36528314784354776No Hit
TCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAC1570.3353769252130818No Hit
GGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTG1460.3118791788605729No Hit
GCGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTG1460.3118791788605729No Hit
CATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGAC1400.29906222630465895No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT1380.294789908786021No Hit
ACATCTCTGCCAAACCCCAAACCTACGCACCGACCTACTGTAGATGACGT1230.2627475273962361No Hit
TCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGAC1150.24565825732168414No Hit
TTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCACTAG1020.21788819345053725No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG960.20507124089462328No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN900.19225428833870933No Hit
TCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGA870.18584581206075237No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG860.18370965330143338No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT840.1794373357827954No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT750.16021190694892443No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC730.15593958943028646No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA720.15380343067096747No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT700.14953111315232948No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG680.1452587956336915No Hit
TATTTTTGGGCTGTTGCTTTTTACTTGTCATTTTTGTGTTGTAAACCCTT610.13030568431845854No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG530.11321641424390662No Hit
CCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACGTGC530.11321641424390662No Hit
ATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACG490.10467177920663064No Hit
GTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGC480.10253562044731164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)