FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003034038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG108.695652173913043No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA76.086956521739131No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT65.217391304347826No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA65.217391304347826No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT54.3478260869565215No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC54.3478260869565215No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA54.3478260869565215No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT43.4782608695652173No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA43.4782608695652173No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA43.4782608695652173No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA32.608695652173913No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA32.608695652173913No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT32.608695652173913No Hit
TCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT32.608695652173913No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA32.608695652173913No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA32.608695652173913No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT21.7391304347826086No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT21.7391304347826086No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA21.7391304347826086No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC21.7391304347826086No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA21.7391304347826086No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT21.7391304347826086No Hit
CGCCCACGGACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT21.7391304347826086No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC21.7391304347826086No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG21.7391304347826086No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT21.7391304347826086No Hit
TACTTGGCCACCTGTAGTACATGAAAACCCAATCCACATCAAAACCCCCT10.8695652173913043No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT10.8695652173913043No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG10.8695652173913043No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGGAATAGCTTCTAAGAAGA10.8695652173913043No Hit
TAGAAGTAGGGTCTTGGTGACAAAATAAGTTGTGTAGAGTTCAGGGGAGA10.8695652173913043No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGTTTGGTGGAAATT10.8695652173913043No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTC10.8695652173913043No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTAATATTGCTTC10.8695652173913043No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC10.8695652173913043No Hit
CCCGTGGTCGATTATGAGAATGACTGCGCCGGTGAAGCTTGAGGGGGTTT10.8695652173913043No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG10.8695652173913043No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT10.8695652173913043No Hit
CAGGTAGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG10.8695652173913043No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA10.8695652173913043No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC10.8695652173913043No Hit
TGACTAGCTTACACAATAGCTTTTACACCGTAGTCACATCGCCACTCATC10.8695652173913043No Hit
TAGAAGTGGGGCTTGGGTGACAAAATTGGTTGTGTAGGGTCCAGGGGAGA10.8695652173913043No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT10.8695652173913043No Hit
AGGGGCAGGTTTTGGCTCGCGAGAAGGCCTAGATAGGGGATTGTGCGGTG10.8695652173913043No Hit
GGGGATAAGCTCTGCTAGGATAGGGGAAGTAGCGTCTTGTAGACCTACTT10.8695652173913043No Hit
ACTCCTACTTATCTCCCCTTTTATATTAATAATCTTATAGAAATTTAGGT10.8695652173913043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)