FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003034039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115
Sequences flagged as poor quality0
Sequence length211-220
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT97.82608695652174No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT76.086956521739131No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG65.217391304347826No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA65.217391304347826No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT54.3478260869565215No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT54.3478260869565215No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT43.4782608695652173No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT43.4782608695652173No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC32.608695652173913No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT32.608695652173913No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC32.608695652173913No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG32.608695652173913No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG32.608695652173913No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT32.608695652173913No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG32.608695652173913No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA21.7391304347826086No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA21.7391304347826086No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC21.7391304347826086No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT21.7391304347826086No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC21.7391304347826086No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG21.7391304347826086No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC21.7391304347826086No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG21.7391304347826086No Hit
TAATTTCACTTTACATCCAAACTTTACTTTGCCTTCGAAGCCGCCGCCTG10.8695652173913043No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAGTAGGTGTTGGTATAGAATG10.8695652173913043No Hit
CCTCCTCAAGTATACGTCAACGACCACTTAACTCAACCCCCTCCGCATTT10.8695652173913043No Hit
TAGGATTGGTGATGTGGGTGCAAGAGTATGATGGGGCGGTGGATGTGGTA10.8695652173913043No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGACTGGTTCTAG10.8695652173913043No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGGCATTAGGAGGG10.8695652173913043No Hit
CATTTTCGGGGTATGGGCCCGATAGCGTATTTAGCTGACCTTACTTTAGG10.8695652173913043No Hit
TTGAGGGATAGGAGGAGAATGGGGGCTAGGTGTATGAACATGAGGGTGTT10.8695652173913043No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCACAT10.8695652173913043No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTAACACC10.8695652173913043No Hit
GCGCAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA10.8695652173913043No Hit
AACATCCTATCATCCGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT10.8695652173913043No Hit
TCCCCGGTAAGGTTGTTTCGGATTGCTTGAATGACTGCTCTGATACCATC10.8695652173913043No Hit
AACTCCTGAGCCAACAACTTCATATGCCTAGCTTACACAATAGCTTTTCT10.8695652173913043No Hit
AATCCTAGTCCTACCCGCCGCAGTACTTATCATTCTATTTCCCCCTCTAT10.8695652173913043No Hit
CGGCTATTTAGGCTTTATGACCCTGAAATAGGAACCAGGTGTTCGATCCA10.8695652173913043No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA10.8695652173913043No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGGTGGTATAGAATG10.8695652173913043No Hit
CGAGCAGTAGCCCAAACAATCTCATACGAAGTTACCCTAGCCATCATTCT10.8695652173913043No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC10.8695652173913043No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTAATAG10.8695652173913043No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG10.8695652173913043No Hit
AAAAAATCCCAAACATATAACTAAACTCCTCCCCCCCAATTGGACCAACC10.8695652173913043No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG10.8695652173913043No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT10.8695652173913043No Hit
CAAGCAATCCTATACAACCGTATCGCCGATATCGGATTCACCCTCGCCTT10.8695652173913043No Hit
TTAGGGGGTCGGAGGAAACCTACGCACCGACCTACTGTAGATGACGTGCA10.8695652173913043No Hit
AACTCCTGAGCCCACAACTTAATATGACTAGCTTACACAATAGCTTTTAT10.8695652173913043No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT10.8695652173913043No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA10.8695652173913043No Hit
CAAGCAATACTATACAACCGTATCAGCGATCACGGTTTAATCCTCCACTT10.8695652173913043No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG10.8695652173913043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)