FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003034221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335969
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG216116.432438707142624No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA170025.060585946917722No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT160084.764725316919121No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA157074.675133717694193No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA135994.04769487661064No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA130533.8851798826677464No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT122583.648550907970676No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT119403.553899318091848No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT118483.526515839258979No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC112143.337807952519429No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA109263.2520857579121882No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA106623.1735070795222176No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG101533.022005006414282No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT98502.9318181141712483No Hit
GATGAACCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC81142.4151037744553814No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT75672.2522911340034346No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA73612.1909759531385338No Hit
CAGGTGGTCAAGTATTTATGGTACCGTGCAATATTCATGGTGGCTGGCAG67632.0129833407248885No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT66611.9826233968014906No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA66401.9763728201113793No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC66331.9742892945480088No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG65771.9576210900410456No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA62631.864160086198429No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC62141.8495754072548358No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT56161.6715827948411908No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG53361.5882417723063738No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA52201.553714777256235No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC47601.4167973830918925No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT42841.2751176447827033No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC38481.1453437668356306No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT37201.1072450136768572No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA37181.1066497206587513No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA36351.0819450604073588No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT32670.9724111450758849No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT29210.8694254529435751No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA28880.8596031181448289No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC25860.7697138724108474No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA25480.7584033050668365No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGTTTGGTGGAAATTT24140.7185186728537454No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA19630.5842800972708792No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG18430.5485625161845289No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT15440.4595662099777063No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT14650.43605213576252566No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG12280.365509913116984No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA8320.24764189553202828No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT7160.21311490048188972No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC6700.19942316106545543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)