FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003034319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322063
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG169555.264497939844068No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA160154.9726295786849155No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA148444.609036120262185No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT140504.362500504559667No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA129534.021883917121805No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT126393.9243874645643864No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC119003.6949292529722446No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT116323.6117157202162313No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA109573.402129397043436No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA106613.3102219131039576No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA105653.280414080474938No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT97263.0199060432275675No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT87152.7059923058532025No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA84512.6240207661233983No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC79452.46690864830794No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG79022.4535572232761913No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT77282.399530526636093No Hit
CAATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCAC73932.295513610691076No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG72112.239002927998559No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT70972.2036061267515983No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA62031.9260206853938515No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA58521.817035797343998No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT52291.6235953835119215No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG48821.5158524884882771No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT44631.385753718992868No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT44331.376438771296299No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG44191.372091795704567No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC43221.3419734648189952No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC42261.3121656321899753No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT42141.3084396531113478No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC40811.2671433849898934No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA39351.221810639533259No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA38801.204733235422883No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA38541.1966602807525235No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT34761.079291939775758No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT34421.0687349990529802No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT33601.0432741420156926No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA32311.0032198669204473No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC30100.9345997522223913No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA27260.8464182473615411No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG20630.6405579032673732No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT18580.5769057606741538No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT14370.44618599466563996No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGTTTGGTGGAAATTT13340.41420467424075413No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA13040.4048897265441854No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG9860.30615128096055744No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA7120.22107475866523008No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC5750.17853649751756645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)