FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003034356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146535
Sequences flagged as poor quality0
Sequence length208-222
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG139769.537653120414918No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT110667.5517794383594365No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC103607.069983280444945No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT51263.498140376019381No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT50203.4258027092503496No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCACCTGGTTCTAG49913.406012215511652No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATTATTCT47163.2183437404033164No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT46423.1678438598287095No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG46193.1521479510014676No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA44523.038182004299314No Hit
GGTTGACCTGTTAGGGTGAGAAGGATTATTCGAGTGCTATAGGCGCTTGT43832.991094277817586No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG43542.971303784078889No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC39162.672399085542703No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC39152.6717166547241273No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT39072.666257208175521No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG31692.1626232640666054No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG31502.1496570785136657No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC30202.060941072098816No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG29031.9810966663254512No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA28211.9251373392022382No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG28001.9108062920121474No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT26391.8009349302214488No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG25851.7640836660183574No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT24361.6624014740505682No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT24341.6610366124134166No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT23521.6050772852902038No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA23351.5934759613744156No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA18761.2802402156481385No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA18221.2433889514450471No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC15901.0850650015354693No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC15841.0809704166240148No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC12940.8830654792370423No Hit
GGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA11570.7895724570921623No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG9260.6319309380011602No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA9250.6312485071825844No Hit
TAATCCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC6590.44972190944143037No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG5050.3446275633807623No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG5030.34326270174361073No Hit
ATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG4770.3255195004606408No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT4250.29003309789470094No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT3380.23066161667860918No Hit
TCGCCGACACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC2980.20336438393557854No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC2150.14672262599378988No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG2070.14126317944518374No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA1960.1337564404408503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)