FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003034491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA66.666666666666667No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA44.444444444444445No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA44.444444444444445No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA44.444444444444445No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG44.444444444444445No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT33.3333333333333335No Hit
TCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT33.3333333333333335No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT33.3333333333333335No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT33.3333333333333335No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA33.3333333333333335No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC33.3333333333333335No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA22.2222222222222223No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT22.2222222222222223No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA22.2222222222222223No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT22.2222222222222223No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC22.2222222222222223No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT22.2222222222222223No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA22.2222222222222223No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC22.2222222222222223No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA22.2222222222222223No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA22.2222222222222223No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT11.1111111111111112No Hit
GATTTAACTGTTGCGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCC11.1111111111111112No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT11.1111111111111112No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGTGGTA11.1111111111111112No Hit
AAGGATACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC11.1111111111111112No Hit
TTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG11.1111111111111112No Hit
AGGGGCAGGTTTTGGCCCGTAAGAAGGCTTGACTAGGGGATTGGGCGGTG11.1111111111111112No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG11.1111111111111112No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGAGAGGAATGGGGTGGGTTTTGTAT11.1111111111111112No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCT11.1111111111111112No Hit
TCAGCAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA11.1111111111111112No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG11.1111111111111112No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA11.1111111111111112No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC11.1111111111111112No Hit
GACATTGGTGTTTTGGTAGTTGAAATACAACAAGGGTTTTTCTTTGCATT11.1111111111111112No Hit
CACTTAAACATATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA11.1111111111111112No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG11.1111111111111112No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG11.1111111111111112No Hit
AGCCTTCTCCACTTCAAGTCAGCTAGGACTCATAATAGTTACAATCGGCA11.1111111111111112No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAACCCCCTC11.1111111111111112No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG11.1111111111111112No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATTATTTCATATTGCTTC11.1111111111111112No Hit
CACTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC11.1111111111111112No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA11.1111111111111112No Hit
TACCTTCTTATTATTTGATCGAGAAATTGCCCTCCTTTTACCCCTACCAT11.1111111111111112No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC11.1111111111111112No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG11.1111111111111112No Hit
GATAAGGGGTGTAGGTGTGACTTGTGGTAAGAAGTGGGCTAGGGCATTTT11.1111111111111112No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT11.1111111111111112No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAGGTGGGCTAGGGCATTTT11.1111111111111112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)